Amniotic fluid cell-derived extracellular matrix and uses thereof

ABSTRACT

Disclosed is a cell-derived extracellular matrix (ECM) derived in vitro from cells isolated from amniotic fluid, and methods of use for the isolation, maintenance, and proliferation of adherent cells including stem cells, as well as for the differentiation of stem cells.

CROSS REFERENCE TO RELATED APPLICATIONS

This application claims the benefit of U.S. Provisional PatentApplication No. 62/740,817, filed Oct. 3, 2018, which is incorporated byreference herein in its entirety.

FIELD OF THE INVENTION

The invention generally relates to cell-derived extracellular matricesand uses thereof including isolation, maintenance, and proliferation ofmammalian cells and differentiation of stem cells.

BACKGROUND

In vitro cell culture is perhaps the most ubiquitous, important, andpoorly understood aspect of all cell biology as well as the developingfields of regenerative medicine and tissue engineering. Firstly, itallows for the observation of cell behavior so that various aspects ofcell function may be studied in detail. Secondly, it allows for increasein numbers of specific cell groups. For basic research, as well as manyclinical applications, it is necessary to achieve large quantities ofrelatively rare cells from small biological samples. In vitro cellculture permits small numbers of cells to be expanded outside the bodyto achieve more relevant numbers. Lastly, it permits the storage ofcells for later use. By expanding cell numbers in vitro, and freezingviable cells for later use, relatively small biological samples canyield cells for multiple experiments over the span of days, months, oreven years.

Despite the omnipresence of cell culture, the effects that in vitroculture has on the native characteristics of the cells it is stillrelatively poorly understood. Many of our current practices have arisennot from deliberate thought, planning, and experimentation, but insteadfrom chance observations. Mammalian cell culture began in the early1900s when, in 1911, Alexis Carrel and Montrose Burrows first publishedan academic paper on the cultivation of mammalian tissues in vitro. Theywere studying the physiology and anatomy of tissues by cutting sectionsof mammalian tissues and placing them on microscope slides. They thennoticed that some cells migrated out of the tissue onto the slide. Theywent on to describe techniques for culturing cells in perpetuity. It nowappears that some of their observations may not have been valid, buttheir work paved the way for modern cell culture.

After the discovery of hematopoietic stem cells (HSCs), groups all overthe world were studying (HSCs). During their culture (in suspension), itwas observed that a sub-population of bone marrow cells stuck to thebottom of the plastic flasks and began to proliferate. These cells werelater recognized to be distinct from HSCs, and were eventually dubbedmesenchymal stem cells (MSCs). Because of this chance observation thatlead to the discovery of MSCs, plastic adherence is still widely used asa defining attribute of MSCs and many other mammalian cell types.

The practice of culturing cells on plastic substrates is problematicbecause there is substantial evidence, that is now widely accepted inthe literature, demonstrating the critical role of the microenvironmentin regulating cell function. The microenvironment has been shown to helpdirect the differentiation of stem and progenitor cells, and regulatethe behavior of mature cell types.

When cells are removed from their native environment to be expanded invitro they lose important cues from their surrounding extracellularmatrix or microenvironment which relay important information to thecells regarding the composition and state of their surroundings. Changesto a cell's microenvironment have a profound effect on the behavior ofthose cells. The current standard for isolation and expansion of mostadherent cells in vitro is to place the cells in culture vesselscomposed of polystyrene (plastic). The polystyrene may have been treatedin some manner to facilitate cell attachment and growth but the surfaceis, in most cases, completely foreign to the cell. In other cases, thesurface may be coated with individual matrix proteins (e.g. fibronectinor collagen) or some combination of proteins. These simple substratesdisregard the complexity of the native microenvironment as well as thecritical role of the microenvironment in normal cell function. The cellwill immediately begin to respond to this foreign environment in amanner that is much different than when the cell is in its nativeenvironment.

Five major approaches are currently employed to address this issue ofculturing cells on plastic substrates:

1. Ignore the problem.—Instead of trying to achieve a desired functionthat matches what would be expected in vivo, a multitude of cell typescan be tested in various media in order to find cells that will exhibita specific desired function without the appropriate matrix substrate.This approach is unsophisticated and often fails to produce desiredresults because of the complex interplay of variables and the breadth ofinteractions between cells and the extracellular matrix.

2. Identify key components.—Many academic laboratories and severalcompanies have taken the approach of considering the tissue from whichcells are isolated and looking for unique elements of that tissue thatmay be important for cell function. Cells are then cultured on simplesubstrates consisting of only one or a few matrix components. Thisapproach often fails because matrices are naturally very complexenvironments including over one-hundred different proteins in somecases. Cells respond just as strongly to signals they need and fail toreceive, as to signals they do not need and do receive.

3. Shotgun approach—The use of protein gels like MATRIGEL™ employs asort of shotgun approach. A gel is created that contains many differentmatrix proteins with the hopes that it will contain the necessarybinding motifs for many different cell types. This approach may fail byproviding cues that push cells in a particular direction or by failingto provide all the cues that cells are expecting.

4. Tissue-derived matrices—This is a biomimetic approach that typicallyinvolves isolating a tissue of interest from a genetically similaranimal, physically disrupting or chemically digesting the tissue toobtain a solution or uniform suspension, and then coating culturevessels with the deconstructed tissue. For example, someone who wishesto culture satellite cells, might collect muscle, homogenize the tissue,and then coat a culture vessel in homogenized muscle prior to seedingthe cells. This method often fails for a few key reasons. Firstly, evenwithin a specific tissue type, the stem cell/progenitor cell niche, maybe distinct from the rest of the tissue. Simply homogenizing muscle doesnot guarantee that an appropriate niche is being created. Secondly, theniche consists of structural and physical cues, in addition tobiochemical cues. Even if many/most of the biochemical cues are presentin a tissue homogenate, the structure has been destroyed, and cells maysense very different mechanical cues. Lastly, manufacturability oftissue derived matrices is dependent on availability of tissues. Thisaffects the total amount of cell culture possible and contributes tolot-to-lot variability.

5. Cell-derived matrices—Cells in culture can be induced to secrete amatrix in their culture vessel. This matrix is the best approximationavailable of the in vivo niche, and can be manufactured in vitro. Cellscan be induced in vitro to elaborate a matrix and then the cells cansubsequently be eliminated from the matrix, for example by usingnon-ionizing detergent to retain structure and chemistry of the matrix.This approach has several key advantages. (1) The matrix structure canbe recreated and left undisturbed. (2) The matrix can be customizedbased on tissue/cell type of interest. (3) The matrix can be specific tostem and progenitor cell niche. (4) The matrix can be manufactured inlarge quantities.

With respect to cell-derived matrices, not all cell types can beefficiently isolated and expanded on any given cell-derived matrix. Infact, pluripotent stem cells (PSCs) appear to have much differentrequirements for a supportive growth substrate than do other types ofcells. It is known that the specific cell type used to produce a matrixwill have an effect on the composition of the matrix, and therefore, thereaction of various cell types to that matrix (see Marinkovic, M. etal., One size does not fit all: developing a cell-specific niche for invitro study of cell behavior. Matrix Biol. 54-55, 426-441 (2016)). Priorwork disclosed in U.S. Pat. No. 8,084,023 has described the productionand composition of an extracellular matrix produced by bone marrowstromal or mesenchymal stem cells (see also Chen, X. et al.,Extracellular Matrix Made by Bone Marrow Cells Facilitates Expansion ofMarrow-Derived Mesenchymal Progenitor Cells and Prevents TheirDifferentiation into Osteoblasts. Journal of Bone and Mineral Research22, 1943-1956 (2007) and Lai, Y. et al., Reconstitution ofmarrow-derived extracellular matrix ex vivo: a robust culture system forexpanding large-scale highly functional human mesenchymal stem cells.Stem cells and development 19, 1095-107 (2010)). This bone marrow cellderived matrix has been shown to support the expansion of other MSCs buthas not been effective for the attachment and growth of other types ofstem cells, specifically, induced pluripotent stem cells (iPSCs). iPSCshave exhibited an expanded potential to form cells and tissues from amuch broader category than MSCs. This represents a particularlyinteresting challenge, because the difficulty of growing a confluentmonolayer of iPSCs in standard culture conditions makes it impracticalto produce a cell-derived matrix from iPSCs. A major limitation ofprevious cell-derived matrices, is that in order to make atissue-specific matrix (e.g., bone marrow matrix from bone marrow MSCs,adipose matrix from adipose MSCs, or endothelial matrix from hUVECs), itis necessary that the target population of cells already be capable ofadhering to the starting substrate. A difficulty with iPSCs, embryonicstem cells (ES), and many other cell types is that they do not readilyadhere to simple substrates.

SUMMARY OF THE INVENTION

The present invention provides a solution to at least some of theaforementioned limitations and deficiencies in the art relating tocell-derived extracellular matrices (ECMs) to support the isolation,expansion and proliferation of pluripotent stem cells (PSCs), includingbut not limited to induced pluripotent stem cells (iPSCs) and embryonicstem cells (ES). The solution is premised on the use of an amnioticfluid cell-derived extracellular matrix. The use of uncommitted, readilyadherent, and highly proliferative perinatal cells found in amnioticfluid allows for the creation of an extracellular matrix (ECM) thatsurprisingly, supports adhesion, isolation, expansion, and proliferationof these PSCs. This technical achievement was not possible with thecell-derived ECMs of the prior art. Additionally, the amniotic fluidcell-derived extracellular matrix (AFC-ECM) of the present invention iseffective for the isolation, expansion, and proliferation of adherentcell types including but not limited to other stem cells such asmesenchymal stem cells, somatic cells, progenitor cells, mature cells,and cells from multiple germ layers. Surprisingly, the AFC-ECM of theinvention supports increased proliferation of MSCs produced by bonemarrow relative to a bone marrow cell-derived ECM. Furthermore, theAFC-ECM of the invention can support differentiation of stem cells intodifferentiated cell types.

In one aspect of the invention, disclosed is a cell-derivedextracellular matrix (ECM) derived in vitro from cells isolated fromamniotic fluid. In some embodiments, the ECM comprises laminin, collagenalpha-1 (XVIII), basement membrane-specific heparan sulfate proteoglycancore protein, agrin, vimentin, and collagen alpha-2 (IV), and/orisoforms thereof. In some embodiments, the isoform of collagen alpha-1(XVIII) is isoform 2. In some embodiments, the isoform of agrin isisoform 6. In some embodiments, the cell-derived ECM further comprisesfibronectin and/or an isoform thereof. In some embodiments, thecell-derived ECM does not contain any one of or all of decorin,perlecan, and collagen (III). In some embodiments, the cells isolatedfrom amniotic fluid comprise stem cells. In some embodiments, thecell-derived ECM is decellularized. In some embodiments, thecell-derived ECM includes any one of, any combination of, or all of thecomponents listed in Table 2 (see Example 2) and/or any variants,derivatives, or isoforms thereof listed in Table 2, preferably all ofthe components listed in Table 2 and/or any derivatives or isoformsthereof.

In another aspect of the invention, disclosed is method of proliferatingadherent cells in culture, the method comprising culturing the adherentcells in the presence of a cell-derived extracellular matrix (ECM) in aculture media thereby proliferating the adherent cells, wherein thecell-derived ECM is derived in vitro from cells isolated from amnioticfluid. In some embodiments, the cell-derived ECM comprises laminin,collagen alpha-1 (XVIII), basement membrane-specific heparan sulfateproteoglycan core protein, agrin, vimentin, and collagen alpha-2 (IV),and/or isoforms thereof. In some embodiments, the isoform of collagenalpha-1 (XVIII) is isoform 2. In some embodiments, the isoform of agrinis isoform 6. In some embodiments, the cell-derived ECM furthercomprises fibronectin and/or an isoform thereof. In some embodiments,the cell-derived ECM does not contain decorin, perlecan, or collagen(III). In some embodiments, the cells isolated from amniotic fluidcomprise stem cells. In some embodiments, the cell-derived ECM isdecellularized. In some embodiments, the adherent cells comprisemammalian adherent cells. In various embodiments, the adherent cellscomprise stem cells, somatic cells, progenitor cells, mature cells, orcells from multiple germ layers. In some embodiments, the adherent cellscomprise stem cells. In some embodiments, the stem cells are maintainedin an undifferentiated state. In some embodiments, the stem cellscomprise pluripotent stem cells (PSCs). In some embodiments, the PSCscomprise induced PSCs (iPSC) and/or embryonic stem cells (ES). In someembodiments, the stem cells comprise mesenchymal stem cells (MSCs). Insome embodiments, the MSCs are obtained from bone marrow. In someembodiments, the adherent cells comprise progenitor cells. In someembodiments, the progenitor cells comprise endothelial progenitor cells.In some embodiments, the adherent cells comprise mature cells. In someembodiments, the mature cells comprise chondrocytes. In someembodiments, the cell-derived ECM includes any one of, any combinationof, or all of the components listed in Table 2 and/or any variants,derivatives, or isoforms thereof listed in Table 2, preferably all ofthe components listed in Table 2 and/or any derivatives or isoformsthereof.

In another aspect of the invention, disclosed is a method of inducingdifferentiation of stem cells into differentiated cell types, the methodcomprising culturing the stem cells in the presence of a cell-derivedextracellular matrix (ECM) in a differentiation media, wherein thecell-derived ECM is derived in vitro from cells isolated from amnioticfluid. In some embodiments, the cell-derived ECM comprises laminin,collagen alpha-1 (XVIII), basement membrane-specific heparan sulfateproteoglycan core protein, agrin, vimentin, and collagen alpha-2 (IV),and/or isoforms thereof. In some embodiments, the isoform of collagenalpha-1 (XVIII) is isoform 2. In some embodiments, the isoform of agrinis isoform 6. In some embodiments, the cell-derived ECM furthercomprises fibronectin and/or an isoform thereof. In some embodiments,the cell-derived ECM does not contain decorin, perlecan, or collagen(III). In some embodiments, the cells isolated from amniotic fluidcomprise stem cells. In some embodiments, the cell-derived ECM isdecellularized. In some embodiments, the stem cells comprise pluripotentstem cells (PSCs). In some embodiments, the PSCs comprise induced PSCs(iPSC). In some embodiments, the PSCs comprise embryonic stem cells(ES). In some embodiments, the stem cells comprise mesenchymal stemcells (MSCs). In some embodiments, the MSCs are obtained from bonemarrow. In some embodiments, the differentiated cell types compriseadipocytes, osteoblasts, chondrocytes, or myocytes. In some embodiments,the cell-derived ECM includes any one of, any combination of, or all ofthe components listed in Table 2 and/or any variants, derivatives, orisoforms thereof listed in Table 2, preferably all of the componentslisted in Table 2 and/or any derivatives or isoforms thereof.

In another aspect of the invention, disclosed is a method of producing acell-derived extracellular matrix (ECM) in vitro, the method comprising:

-   -   (a) isolating cells from amniotic fluid,    -   (b) seeding the isolated cells onto a cell culture container or        onto a cell culture container coated with a substrate,    -   (c) adding a culture media to the cell culture container, and    -   (d) culturing the cells, thereby producing a cell-derived ECM,        and    -   (e) optionally decellularizing the cell-derived ECM.        In some embodiments, the isolated cells from the amniotic fluid        comprise stem cells. In some embodiments, the substrate is        fibronectin. In some embodiments, the cell-derived ECM comprises        laminin, collagen alpha-1 (XVIII), basement membrane-specific        heparan sulfate proteoglycan core protein, agrin, vimentin, and        collagen alpha-2 (IV), and/or isoforms thereof. In some        embodiments, the isoform of collagen alpha-1 (XVIII) is        isoform 2. In some embodiments, the isoform of agrin is        isoform 6. In some embodiments, the cell-derived ECM further        comprises fibronectin and/or an isoform thereof. In some        embodiments, the cell-derived ECM does not contain decorin,        perlecan, or collagen (III). In some embodiments, the        cell-derived ECM includes any one of, any combination of, or all        of the components listed in Table 2 and/or any variants,        derivatives, or isoforms thereof listed in Table 2.

In another aspect of the invention, disclosed is an amniotic fluidcell-derived extracellular matrix (AFC-ECM) produced in vitro by themethod comprising:

-   -   (a) isolating cells from amniotic fluid,    -   (b) seeding the isolated cells onto a cell culture container or        onto a cell culture container coated with a substrate,    -   (c) adding a culture media to the cell culture container, and    -   (d) culturing the cells, thereby producing the AFC-ECM, and    -   (e) optionally decellularizing the AFC-ECM.        In some embodiments, the isolated cells from the amniotic fluid        comprise stem cells. In some embodiments, the substrate is        fibronectin. In some embodiments, the AFC-ECM comprises laminin,        collagen alpha-1 (XVIII), basement membrane-specific heparan        sulfate proteoglycan core protein, agrin, vimentin, and collagen        alpha-2 (IV), and/or isoforms thereof. In some embodiments, the        isoform of collagen alpha-1 (XVIII) is isoform 2. In some        embodiments, the isoform of agrin is isoform 6. In some        embodiments, the AFC-ECM further comprises fibronectin and/or an        isoform thereof. In some embodiments, the AFC-ECM does not        contain decorin, perlecan, or collagen (III). In some        embodiments, the AFC-ECM includes any one of, any combination        of, or all of the components listed in Table 2 and/or any        variants, derivatives, or isoforms thereof listed in Table 2.

In another aspect of the invention, disclosed is a cell-derivedextracellular matrix (ECM) derived in vitro from cells isolated from anumbilical cord. In some embodiments, the cells isolated from theumbilical cord are from the cord blood and/or the Wharton's jelly.

In another aspect of the invention, disclosed is a cell-derivedextracellular matrix (ECM) derived in vitro from cells isolated fromplacenta tissue. In some embodiments, the cells isolated from theplacenta tissue are from the membrane sheets (amnion and/or chorion),the villi, and/or the blood.

Also disclosed in the context of the present invention are embodiments 1to 53. Embodiment 1 is a cell-derived extracellular matrix (ECM) derivedin vitro from cells isolated from amniotic fluid. Embodiment 2 is thecell-derived ECM of embodiment 1, wherein the ECM comprises laminin,collagen alpha-1 (XVIII), basement membrane-specific heparan sulfateproteoglycan core protein, agrin, vimentin, and collagen alpha-2 (IV),and/or isoforms thereof. Embodiment 3 is the cell-derived ECM ofembodiment 2, wherein the isoform of collagen alpha-1 (XVIII) 15 isoform2, and/or wherein the isoform of agrin is isoform 6. Embodiment 4 is thecell derived ECM of any one of embodiments 2 or 3, wherein thecell-derived ECM further comprises fibronectin and/or an isoformthereof. Embodiment 5 is the cell-derived ECM of any one of embodiments1 to 4, wherein the cell-derived ECM does not contain decorin, perlecan,and/or collagen (III). Embodiment 6 is the cell-derived ECM of any oneof embodiments 1 to 5, wherein the cells isolated from amniotic fluidcomprise stem cells. Embodiment 7 is the cell-derived ECM of any one ofembodiments 1 to 6, wherein the cell-derived ECM is decellularized.

Embodiment 8 is a method of proliferating adherent cells in culture, themethod comprising culturing the adherent cells in the presence of acell-derived extracellular matrix (ECM) in a culture media therebyproliferating the adherent cells, wherein the cell-derived ECM isderived in vitro from cells isolated from amniotic fluid. Embodiment 9is the method of embodiment 8, wherein the cell-derived ECM compriseslaminin, collagen alpha-1 (XVIII), basement membrane-specific heparansulfate proteoglycan core protein, agrin, vimentin, and collagen alpha-2(IV), and/or isoforms thereof. Embodiment 10 is the method of embodiment9, wherein the isoform of collagen alpha-1 (XVIII) is isoform 2, and/orwherein the isoform of agrin is isoform 6. Embodiment 11 is the methodof any one of embodiments 9 or 10, wherein the cell-derived ECM furthercomprises fibronectin and/or an isoform thereof. Embodiment 12 is themethod of any one of embodiments 8 to 11, wherein the cell-derived ECMdoes not contain decorin, perlecan, and/or collagen (III). Embodiment 13is the method of any one of embodiments 8 to 12, wherein the cellsisolated from amniotic fluid comprise stem cells. Embodiment 14 is themethod of any one of embodiments 8 to 13, wherein the cell-derived ECMis decellularized prior to contact with the adherent stem cells.Embodiment 15 is the method of any one of embodiments 8 to 14, whereinthe adherent cells comprise mammalian adherent cells. Embodiment 16 isthe method of any one of embodiments 8 to 15, wherein the adherent cellscomprise stem cells, somatic cells, progenitor cells, mature cells, orcells from multiple germ layers. Embodiment 17 is the method ofembodiment 16, wherein the adherent cells comprise stem cells.Embodiment 18 is the method of embodiment 17, wherein the stem cells aremaintained in an undifferentiated state. Embodiment 19 is the method anyone of embodiments 17 or 18, wherein the stem cells comprise pluripotentstem cells (PSCs). Embodiment 20 is the method of embodiment 19, whereinthe PSCs comprise induced PSCs (iPSC). Embodiment 21 is the method ofembodiment 19, wherein the PSCs comprise embryonic stem cells (ES).Embodiment 22 is the method of any one of embodiments 17 or 18, whereinthe stem cells comprise mesenchymal stem cells (MSCs). Embodiment 23 isthe method of embodiment 22, wherein the MSCs are obtained from bonemarrow. Embodiment 24 is the method of embodiment 16, wherein theadherent cells comprise progenitor cells. Embodiment 25 is the method ofembodiment 24, wherein the progenitor cells comprise endothelialprogenitor cells. Embodiment 26 is the method of embodiment 16, whereinthe adherent cells comprise mature cells. Embodiment 27 is the method ofembodiment 26, wherein the mature cells comprise chondrocytes.

Embodiment 28 is a method of inducing differentiation of stem cells intodifferentiated cell types, the method comprising culturing the stemcells in the presence of a cell-derived extracellular matrix (ECM) in adifferentiation media, wherein the cell-derived ECM is derived in vitrofrom cells isolated from amniotic fluid. Embodiment 29 is the method ofembodiment 28, wherein the cell-derived ECM comprises laminin, collagenalpha-1 (XVIII), basement membrane-specific heparan sulfate proteoglycancore protein, agrin, vimentin, and collagen alpha-2 (IV), and/orisoforms thereof. Embodiment 30 is the method of embodiment 29, whereinthe isoform of collagen alpha-1 (XVIII) is isoform 2, and/or wherein theisoform of agrin is isoform 6. Embodiment 31 is the method of any one ofembodiments 29 or 30, wherein the cell-derived ECM further comprisesfibronectin and/or an isoform thereof. Embodiment 32 is the method ofany one of embodiments 28 to 31, wherein the cell-derived ECM does notcontain decorin, perlecan, and/or collagen (III). Embodiment 33 is themethod of any one of embodiments 28 to 32, wherein the cells isolatedfrom amniotic fluid comprise stem cells. Embodiment 34 is the method ofany one of embodiments 28 to 33, wherein the stem cells comprisepluripotent stem cells (PSCs). Embodiment 35 is the method of embodiment34, wherein the PSCs comprise induced PSCs (iPSC). Embodiment 36 is themethod of embodiment 34, wherein the PSCs comprise embryonic stem cells(ES). Embodiment 37 is the method of any one of embodiments 28 to 33,wherein the stem cells comprise mesenchymal stem cells (MSCs).Embodiment 38 is the method of embodiment 22, wherein the MSCs areobtained from bone marrow. Embodiment 39 is the method of any one ofembodiments 37 or 38, wherein the differentiated cell types compriseadipocytes, osteoblasts, chondrocytes, or myocytes.

Embodiment 40 is a method of producing a cell-derived extracellularmatrix (ECM) in vitro, the method comprising: (a) isolating cells fromamniotic fluid; (b) seeding the isolated cells onto a cell culturecontainer or onto a cell culture container coated with a substrate; (c)adding a culture media to the cell culture container; and (d) culturingthe cells, thereby producing a cell-derived ECM; and (e) optionallydecellularizing the cell-derived ECM. Embodiment 41 is the method ofembodiment 40, wherein the isolated cells from the amniotic fluidcomprise stem cells. Embodiment 42 is the method of embodiment 40 or 41,wherein the substrate is fibronectin. Embodiment 43 is the method of anyone of embodiments 40 to 42, wherein the cell-derived ECM compriseslaminin, collagen alpha-1 (XVIII), basement membrane-specific heparansulfate proteoglycan core protein, agrin, vimentin, and collagen alpha-2(IV), and/or isoforms thereof. Embodiment 44 is the method of embodiment43, wherein the isoform of collagen alpha-1 (XVIII) is isoform 2, and/orwherein the isoform of agrin is isoform 6. Embodiment 45 is the methodof any one of embodiments 43 or 44, wherein the cell-derived ECM furthercomprises fibronectin and/or an isoform thereof. Embodiment 46 is themethod of any one of embodiments 40 to 45, wherein the cell-derived ECMdoes not contain decorin, perlecan, and/or collagen (III).

Embodiment 47 is an amniotic fluid cell-derived extracellular matrix(AFC-ECM) produced in vitro by the method comprising: (a) isolatingcells from amniotic fluid, (b) seeding the isolated cells onto a cellculture container or onto a cell culture container coated with asubstrate, (c) adding a culture media to the cell culture container, and(d) culturing the cells, thereby producing the AFC-ECM, and (e)optionally decellularizing the AFC-ECM. Embodiment 48 is the method ofembodiment 47, wherein the isolated cells from the amniotic fluidcomprise stem cells. Embodiment 49 is the method of embodiment 47 or 48,wherein the substrate is fibronectin. Embodiment 50 is the method of anyone of embodiments 47 to 49, wherein the cell-derived ECM compriseslaminin, collagen alpha-1 (XVIII), basement membrane-specific heparansulfate proteoglycan core protein, agrin, vimentin, and collagen alpha-2(IV), and/or isoforms thereof. Embodiment 51 is the method of anyembodiment 50, wherein the isoform of collagen alpha-1 (XVIII) isisoform 2, and/or wherein the isoform of agrin is isoform 6. Embodiment52 is the method of any one of embodiments 50 or 51, wherein thecell-derived ECM further comprises fibronectin and/or an isoformthereof. Embodiment 53 is the method of any one of embodiments 47 to 52,wherein the cell-derived ECM does not contain decorin, perlecan, and/orcollagen (III).

The term “stem-like characteristic” or “stemness” refers acharacteristic that can be observed in or associated with a stem cell.For example, one of the stem-like characteristics can be chosen from anability of differentiating into different cell types, an ability ofself-renewal, an ability of survival under certain conditions (includinghypoxia conditions), an ability to resist chemotherapeutic agents, andan expression of a wide variety of markers.

The term “isoform” refers to different forms of a biomolecule or aprotein. The different forms of the biomolecule or protein may beproduced by a variety of processes or mechanisms. In embodiments inwhich the biomolecule is a protein, the isoforms may be proteins thatdiffer in sequence by one or more amino acids. For example, the proteinisoforms may be genetic alleles. Alternatively, the protein isoforms maybe the products of alternate splicing, RNA editing, posttranslationalprocessing, and the like.

The terms “about” or “approximately” are defined as being close to asunderstood by one of ordinary skill in the art, and in one non-limitingembodiment the terms are defined to be within 10%, preferably within 5%,more preferably within 1%, and most preferably within 0.5%.

The term “substantially” and its variations are defined to includeranges within 10%, within 5%, within 1%, or within 0.5%.

The terms “wt. %,” “vol. %,” or “mol. %” refers to a weight percentageof a component, a volume percentage of a component, or molar percentageof a component, respectively, based on the total weight, the totalvolume of material, or total moles, that includes the component. In anon-limiting example, 10 grams of component in 100 grams of the materialis 10 wt. % of component.

The terms “inhibiting” or “reducing” or “preventing” or “avoiding” orany variation of these terms, when used in the claims and/or thespecification includes any measurable decrease or complete inhibition toachieve a desired result.

The term “effective,” as that term is used in the specification and/orclaims, means adequate to accomplish a desired, expected, or intendedresult.

The words “comprising” (and any form of comprising, such as “comprise”and “comprises”), “having” (and any form of having, such as “have” and“has”), “including” (and any form of including, such as “includes” and“include”) or “containing” (and any form of containing, such as“contains” and “contain”) are inclusive or open-ended and do not excludeadditional, unrecited elements or method steps.

The use of the word “a” or “an” when used in conjunction with the terms“comprising,” “having,” “including,” or “containing” (or any variationsof these words) may mean “one,” but it is also consistent with themeaning of “one or more,” “at least one,” and “one or more than one.”

The compositions and methods for their use can “comprise,” “consistessentially of,” or “consist of” any of the ingredients or stepsdisclosed throughout the specification. With respect to the transitionalphrase “consisting essentially of,” in one non-limiting aspect, a basicand novel characteristic of the cell-derived extracellular matrix of thepresent invention is that it (1) is derived in vitro from cells isolatedfrom amniotic fluid and (2) provides an environment that supportsadhesion, isolation, expansion, and/or proliferation of PSCs.

It is contemplated that any embodiment discussed in this specificationcan be implemented with respect to any method or composition of theinvention, and vice versa. Furthermore, compositions of the inventioncan be used to achieve methods of the invention.

Other objects, features and advantages of the present invention willbecome apparent from the following detailed description. It should beunderstood, however, that the detailed description and the specificexamples, while indicating specific embodiments of the invention, aregiven by way of illustration only, since various changes andmodifications within the spirit and scope of the invention will becomeapparent to those skilled in the art from this detailed description.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1: a photomicrograph of a Brightfield Image of the amniotic fluidcell-derived ECM at 100× power using a 10× objective lens

FIG. 2: an atomic force photomicrograph of 3 representative 40×40 umsections of the amniotic fluid cell-derived ECM and a bone marrowcell-derived ECM showing topography, adhesion, and stiffness.

FIG. 3a : a scatter plot showing quantification of adhesion of bonemarrow- and amniotic fluid-cell-derived ECMs. Each point represents anindependent point of measurement.

FIG. 3b : a scatter plot showing quantification of stiffness (elasticmodulus) of bone marrow- and amniotic fluid-cell-derived ECMs. Eachpoint represents an independent point of measurement.

FIG. 4: a photomicrograph showing Day 0 and Day 2 culture of iPSCs onamniotic fluid cell-derived ECM and a bone marrow cell-derived ECM.

FIG. 5: a plot of growth curves of iPSCs cultured in the presence of theamniotic fluid cell-derived ECM and a bone marrow cell-derived ECM.

FIG. 6: a bar graph of pluripotent transcription factor expression iniPSCs cultured on amniotic fluid cell-derived ECM.

FIG. 7: photomicrographs of Brightfield Images of MSCs, EPCs, andChondrocytes in culture on amniotic fluid cell-derived ECM vs. bonemarrow ECM vs. tissue culture.

FIG. 8a : a bar graph quantifying the proliferation of MSCs in cultureafter 4 days on amniotic fluid cell-derived ECM.

FIG. 8b : a bar graph quantifying the proliferation of EPCs in cultureafter 4 days on amniotic fluid cell-derived ECM.

FIG. 8c : a bar graph quantifying the proliferation of chondrocytes inculture after 4 days on amniotic fluid cell-derived ECM.

FIG. 9: a bar graph of the percentage of MSCs expressing SSEA-4 asmeasured by flow cytometry following culture on amniotic fluidcell-derived ECM vs. bone marrow cell ECM vs. tissue culture plastic forfour days

FIG. 10: a photomicrograph of adipogenic differentiation of MSCscultured on amniotic fluid cell-derived matrix for four days.

FIG. 11: a photomicrograph of osteogenic differentiation of MSCscultured on amniotic fluid cell-derived matrix for four days.

DETAILED DESCRIPTION OF THE INVENTION

The present invention discloses a cell-derived extracellular matrix(ECM) derived in vitro from cells isolated from amniotic fluid andmethods of making the ECM. Also disclosed are methods of using the ECMfor the isolation, maintenance, and expansion/proliferation of adherentcells which can be mammalian cells.

The function of mammalian cells is determined, largely, by theenvironment, e.g., an extracellular matrix, in which they reside. Theyreact to signals that are present in their environment (positivesignals) and also to signals that are required but are not present(negative signals). It is likely that uncommitted stem cells can producea matrix that contains niche motifs necessary to maintain stem cellviability and stemness, but lack many lineage specific signals that moremature cells may secrete which would push a stem cell toward aparticular fate. Without being bound by theory, it is suggested that aless mature cell, e.g., a perinatal cell or perinatal stem cell, mayproduce an ECM that is different from ECMs disclosed previously in theart, such as bone marrow stromal cell-derived ECMs, and may allow forbetter isolation and expansion/proliferation of stem cells with higherpotential than mesenchymal stem cells (MSCs), such as pluripotent stemcells (PSCs). Mass spectrometry demonstrated, that compared topreviously known cell-derived ECMs, the amniotic fluid cell-derived ECMof the invention contains matrix proteins found in all 3 germ layers andlacked specific proteins strongly associated with osteogenic lineages.Moreover, the ECM of the invention contained specific motifs, such aslaminin, that are known to facilitate pluripotent cell adhesion andexpansion.

A. Amniotic Fluid Cell-Derived Extracellular Matrix (AFC-ECM)

Perinatal cells can be divided into three groups: cells from amnioticfluid; cells from the placenta; and cells from the umbilical cord.Amniotic fluid has several sources of cells including cells derived fromthe developing fetus sloughed from the fetal amnion membrane, skin, andalimentary, respiratory, and urogenital tracts. Placenta also hasseveral sources of cells including the membrane sheets (amnion andchorion), the villi, and the blood. Umbilical cord cells generally comefrom two sources, cord blood and Wharton's jelly. The cells from thesethree perinatal sources can include stem cells. The cells used toproduce the amniotic fluid cell-derived ECM of the invention areobtained from the amniotic fluid of a mammal including but not limitedto a human (Homo sapiens), murine, rabbit, cat, dog, pig, equine, orprimate. In preferred embodiments, the cells are from the amniotic fluidof a human. The amniotic fluid can be sourced from humans at full-termbirths (greater than about 37 weeks gestational age) or pre-term births(less than about 37 weeks gestational age). Pre-term births include latepre-term births (about 33 to about 37 weeks gestational age) moderatepre-term births (about 29 to about 33 weeks gestational age), andextreme pre-term births (about 23 to about 29 weeks gestational age).The amniotic fluid can be sourced from humans prior to birth at anygestational age where amniotic fluid is present, and can be combinedwith sources of amniotic fluid from births. Generally, prior to birth,amniotic fluid is collected by an amniocentesis procedure. In someembodiments the amniotic fluid is sourced from humans at full-termbirths, at pre-term births, at late pre-term births, at moderatepre-term births, at extreme pre-term births, or prior to birth, orcombinations thereof. In some embodiments, the amniotic fluid is sourcedprior to birth and is collected from about 10 weeks gestational age upto birth, or from about 10 weeks to about 23 weeks gestational age, orfrom about 10 weeks to about 16 weeks gestational age, or from about 12weeks gestational age up to birth, or from about 12 weeks to about 23weeks gestational age, or from about 12 weeks to about 16 weeksgestational age. In some embodiments, the amniotic fluid sourced priorto birth is collected by an amniocentesis procedure. The cells can beobtained and isolated from amniotic fluid by techniques known in theart, such as those disclosed in Murphy et. al., Amniotic Fluid StemCells, Perinatal Stem Cells, Second Ed. 2013.

Amniotic fluid is comprised of cells having the ability to differentiateinto cell types derived from all 3 embryonic germ layers (ectoderm,endoderm, mesoderm) spontaneously or as a result of treatment withspecific growth factors or combinations of growth factors known to oneof skill in the art. That is, a single cell has the capacity to beinduced to express genes which are specific to any of the three germlayers. Amniotic fluid also contains a mixture of different cell typesincluding cells derived from the developing fetus sloughed from thefetal amnion membrane, skin, and alimentary, respiratory, and urogenitaltracts. Because of the origin of the amniotic fluid and placentalmembranes, these cells can maintain highly multipotent differentiationpotential and comprise a cell population that contains cells of allthree germ layers. The amniotic fluid cells can comprise stem cells. Insome embodiments, the amniotic fluid cells are isolated stem cells. Insome embodiments, the amniotic fluid cells comprise stem cells havingthe ability to differentiate into cell types derived from all 3embryonic germ layers (ectoderm, endoderm, mesoderm) and/or multipotentstem cells, and/or pluripotent stem cells.

The amniotic fluid cell-derived ECM of the invention is comprised ofvarious proteins. The proteins of the ECM can be identified bytechniques known in the art and include mass spectroscopy andimmunohistochemical staining. The ECM can include, but is not limited tothe components listed in Table 2 (see Example 2 below) and any variants,derivatives, or isoforms thereof. The amniotic fluid-cell derived ECMcan include any combination of any of the components and any variants,derivatives, or isoforms thereof from Table 2. In some embodiments, acombination can comprise, consist essentially of, or consist of:laminin, collagen alpha-1 (XVIII), basement membrane-specific heparansulfate proteoglycan core protein, agrin, vimentin, and collagen alpha-2(IV), and/or isoforms thereof. In some embodiments, the isoform ofcollagen alpha-1 (XVIII) is isoform 2. In some embodiments, the isoformof agrin is isoform 6. In some embodiments, the cell-derived ECM furthercomprises, consist essentially of, or consists of fibronectin and/or anisoform thereof. In some embodiments, the amniotic fluid cell-derivedECM does not contain any one of or all of decorin, perlecan, andcollagen (III). The most abundant collagens in Table 2 are collagens I,IV, and XVIII. Some noteworthy differences in proteins between theamniotic fluid cell-derived ECM of the present inventions and a bonemarrow cell-derived matrix are described in Table 1.

TABLE 1 Differences Between the Amniotic Fluid Cell-Derived ECM(AFC-Matrix) and a Bone Marrow Cell-Derived Matrix (BM-Matrix)Difference Protein/Gene between AFC-Matrix & Code BM-Matrix PhysiologicRelevance Laminin 5 sub-units are abundant Laminin is known to in AFCMatrix; Low support adhesion and expression in BM-Matrix expansion ofpluripotent cells. Collagen XVIII Abundant in AFC Matrix; Important forocular Absent in BM-Matrix development Agrin Present in AFC Matrix;produced by motoneurons Absent in BM-Matrix to induce aggregation ofacetylcholine receptors Biglycan Abundant in BM-Matrix; Regulates boneand muscle Low expression in AFC development Matrix Collagen IOverexpressed in BM- Key to fibrillar proteins; Matrix relative to AFChighly abundant in bone Matrix Periostin Present in BM-Matrix; Regulatesmineralization; Absent in AFC Matrix Marker of noncardiomyocyte lineagecells in heart

The amniotic fluid cell-derived ECM (AFC-ECM) of the invention can beproduced in vitro by the following process:

-   -   (a) isolating cells from amniotic fluid,    -   (b) seeding the isolated cells onto a cell culture container or        onto a cell culture container coated with a substrate,    -   (c) adding a culture media to the cell culture container, and    -   (d) culturing the cells, thereby producing the AFC-ECM, and    -   (e) optionally decellularizing the AFC-ECM.

Disclosed herein is a method of producing in vitro a cell-derivedextracellular matrix (ECM), the method comprising:

-   -   (a) isolating cells from amniotic fluid,    -   (b) seeding the isolated cells onto a cell culture container or        onto a cell culture container coated with a substrate,    -   (c) adding a culture media to the cell culture container, and    -   (d) culturing the cells, thereby producing a cell-derived ECM,        and    -   (e) optionally decellularizing the cell-derived ECM.

Disclosed herein is an amniotic fluid cell-derived extracellular matrix(AFC-ECM) produced in vitro by the method comprising:

-   -   (a) isolating cells from amniotic fluid,    -   (b) seeding the isolated cells onto a cell culture container or        onto a cell culture container coated with a substrate,    -   (c) adding a culture media to the cell culture container, and    -   (d) culturing the cells, thereby producing the AFC-ECM, and    -   (e) optionally decellularizing the AFC-ECM.

Any seeding density may be used which allows cells to form a confluentmonolayer immediately or after a period of time in culture. In someembodiments, the seeding density is about 10 cells/cm²-about 100,000cells/cm², or about 100 cells/cm²-about 75,000 cells/cm², or about 500cells/cm²-about 50,000 cells/cm², or about 500 cells/cm²-about 10,000cells/cm², or about 500 cells/cm²-about 5,000 cells/cm², or about 500cells/cm²-about 2,500 cells/cm², or about 1,000 cells/cm²-about 25,000cells/cm², or about 2,000 cells/cm²-about 10,000 cells/cm², or about3,000 cells/cm²-about 5000 cells/cm².

Any type of container suitable for cultivation of cells can be used forthe present invention. Examples include, but are not limited to cellculture flasks, T-flasks, stirred flasks, spinner flasks, fermenters,and bioreactors. Rocking bottles, shaking flasks, tubes, and othercontainers are also suitable containers when placed on a rockingplatform or shaker. The cell culture container can be coated with asubstrate to allow for better cell adhesion. A non-limiting example of asuitable substrate for coating the cell container is fibronectin.

Various commercially available cell culture media, e.g., alpha MinimumEssential Media (α-MEM) culture media (Thermo Fisher Scientific, GrandIsland, N.Y.), are suitable for culturing amniotic fluid cells. Thecommercially available culture media can be modified by adding varioussupplemental substances to the media, e.g. sodium bicarbonate,L-glutamine, penicillin, streptomycin, Amphotericin B and/or serum. Theserum can be fetal bovine serum. The media can also be serum free.Additionally, substances such as L-ascorbic acid can be added to themedia or modified media to induce cell production of an ECM.

The initial culture media can be changed and/or replaced with anothermedia at various times during the culturing process. For example, theinitial media can be a “Complete Media” and then be replaced by an“Inducing Media” during the culturing process. A non-limiting example ofa “Complete Media” contains (α-MEM) plus 2 mM L-Glutamine plusantibiotic-antimycotic plus 15% Fetal Bovine Serum. A non-limitingexample of an “Inducing Media” contains the “Complete Media” plus 50 mML-Ascorbic Acid.

The culturing of the amniotic fluid cells can take place in an incubatorat 37° C., 5% CO₂, and 90% humidity. Culturing can take place undervarious environmental conditions including, but not limited to normoxic,i.e., 20-21% oxygen in the atmosphere, or hypoxic conditions.

Decellularizing the amniotic fluid cell-derived ECM of the amnioticfluid cells can include removing the viable amniotic fluid cells orrendering the amniotic fluid cells non-viable. The amniotic fluid cellscan be decellularized from the ECM by using methods known in the art andcan include, but are not limited to lysing the amniotic fluid cells andthen removing the lysed amniotic fluid cells by washing. Varioussubstances can be used to remove the amniotic fluid cells from the ECM.Non-limiting examples include an “Extraction Buffer” containing TRITONX-100 and ammonium hydroxide in PBS buffer. After the ECM has beendecellularized of amniotic fluid cells, the resulting ECM is therebyessentially cell-free or free of viable amniotic fluid cells. If feedercells are used, then the decellularizing methods also apply to anyviable feeder cells present on the ECM, thereby resulting in the ECMbeing essentially free or free of viable feeder cells. Thedecellularizing methods also apply to any viable cells present on theECM, thereby resulting in the ECM being essentially free or free of anyviable cells. Thus, a decellularized ECM means that the ECM isacellular, meaning that the ECM is free of any viable cells.

In some embodiments, the amniotic fluid cell-derived ECM is athree-dimensional (3D) ECM.

The methods described supra also apply to producing cell-derived ECMsfrom other perinatal cells such as cells from the umbilical cordincluding the cord blood and Wharton's jelly; and cells from placentatissue including the membrane sheets (amnion and chorion), the villi andthe blood.

In one embodiment, a perinatal cell-derived ECM is produced in vitro bythe following process:

-   -   (a) isolating cells from an umbilical cord,    -   (b) seeding the isolated cells onto a cell culture container or        onto a cell culture container coated with a substrate,    -   (c) adding a culture media to the cell culture container, and    -   (d) culturing the cells, thereby producing a cell-derived ECM,        and    -   (e) optionally decellularizing the cell-derived ECM.        In some embodiments, the cells isolated from the umbilical cord        are from the cord blood and/or the Wharton's jelly.

In another embodiment, a perinatal cell-derived ECM is produced in vitroby the following process:

-   -   (a) isolating cells from placenta tissue,    -   (b) seeding the isolated cells onto a cell culture container or        onto a cell culture container coated with a substrate,    -   (c) adding a culture media to the cell culture container, and    -   (d) culturing the cells, thereby producing a cell-derived ECM,        and    -   (e) optionally decellularizing the cell-derived ECM.        In some embodiments, the cells isolated from the placenta tissue        are from the membrane sheets (amnion and/or chorion), the villi,        and/or the blood.

In one aspect of the invention, disclosed is a cell-derivedextracellular matrix (ECM) derived in vitro from cells isolated from anumbilical cord. In some embodiments, the cells isolated from theumbilical cord are from the cord blood and/or the Wharton's jelly.

In another aspect of the invention, disclosed is a cell-derivedextracellular matrix (ECM) derived in vitro from cells isolated fromplacenta tissue. In some embodiments, the cells isolated from theplacenta tissue are from the membrane sheets (amnion and/or chorion),the villi, and/or the blood

B. Methods to Expand/Proliferate Adherent Cells

Methods to expand/proliferate adherent cells include obtaining theadherent cells and culturing them in the presence of the amniotic fluidcell-derived ECM of the invention. In some embodiments, the adherentcells are mammalian adherent cells. Any seeding density may be usedwhich allows cells to form a confluent monolayer immediately or after aperiod of time in culture. In some embodiments, the seeding density isabout 10 cells/cm²-about 100,000 cells/cm², or about 100 cells/cm²-about75,000 cells/cm², or about 500 cells/cm²-about 50,000 cells/cm², orabout 500 cells/cm²-about 10,000 cells/cm², or about 500 cells/cm²-about5,000 cells/cm², or about 500 cells/cm²-about 2,500 cells/cm², or about1,000 cells/cm²-about 25,000 cells/cm², or about 2,000 cells/cm²-about10,000 cells/cm², or about 3,000 cells/cm²-about 5000 cells/cm². Invarious embodiments, the adherent cells are stem cells, somatic cells,progenitor cells, mature cells, and/or cells from multiple germ layers.

In some embodiments, the adherent cells are stem cells. In someembodiments, the stem cells are pluripotent stem cells (PSCs) ormesenchymal stem cells (MSCs). Pluripotent stem cells (PSCs) canself-renew and differentiate into any of the three germ layers:ectoderm, endoderm, and mesoderm, from which all tissues and organsdevelop. Embryonic stem cells (ES) are currently the only known naturalpluripotent stem cells. Induced pluripotent stem (iPSCs) cells also arePSCs. iPSCs are derived from cells generally taken from adult tissue oradult cells, and reprogrammed to the level of embryonic stem cells.Methods for producing iPSCs are known in the art. Mesenchymal stem cells(MSCs) are multipotent stromal cells. MSCs can be obtained from thefollowing non-limiting sources: bone marrow, umbilical cord tissue, e.g.Wharton's jelly, umbilical cord blood, adipose tissue, and amnioticfluid. In some embodiments the stem cells are maintained in anundifferentiated state and maintain their stemness.

In some embodiments, the adherent cells are somatic cells also known asvegetal cells, which are any biological cells in a multicellularorganism other than reproductive cells, gametes, germ cells,gametocytes, or undifferentiated stem cells.

In some embodiments, the adherent cells are cells from multiple germlayers including cells from the ectoderm, endoderm, and/or mesoderm.

In some embodiments, the adherent cells are progenitor cells includingbut not limited to endothelial progenitor cells (EPCs), angioblasts,pancreatic progenitor cells, progenitor cells from the periosteum, andbone marrow stromal cells.

In some embodiments, the adherent cells are mature cells including butnot limited to chondrocytes and osteoblasts.

Cell culture techniques suitable for proliferation of adherent cells inculture are known in the art and can be followed. Suitable commerciallyavailable culture media for cell proliferation includes, but is notlimited to: StemMACS™ iPS-Brew XF available from Miltenyl Biotec; alphaminimum essential media (aMEM) which can be supplemented with fetalbovine serum, antibiotic-antimycotic (anti-anti), and/or GlutaMax™available from Gibco; and Iscove's Modified Dulbecco's Medium (IMDM)which can be supplemented with fetal bovine serum,antibiotic-antimycotic (anti-anti), GlutaMax and/or growth factors suchas EGF and FGF. In some embodiments, no Rock inhibitor is used. Oncecells begin to approach confluence (e.g., as determined by brightfieldmicroscopy), cells can be passaged manually, by cutting large coloniesinto smaller colonies and then re-plate those by physically lifting themoff the dish and placing them on a fresh plate of amniotic fluidcell-derived ECM. This procedure can be repeated indefinitely. In someembodiments disclosed is a method of proliferating adherent cells inculture, the method comprising culturing the cells in the presence of acell-derived extracellular matrix (ECM) in a culture media therebyproliferating the adherent cells, wherein the cell-derived ECM isderived in vitro from cells isolated from amniotic fluid.

The methods of expanding/proliferating adherent cells described supraalso apply to the use of expanding/proliferating adherent cells inculture in the presence of other perinatal cell-derived ECMs. In someaspects, disclosed is a method of proliferating adherent cells inculture, the method comprising culturing the adherent cells in thepresence of a cell-derived extracellular matrix (ECM) in a culture mediathereby proliferating the adherent cells, wherein the cell-derived ECMis derived in vitro from cells isolated from an umbilical cord orplacenta tissue. In some embodiments, the cells isolated from theumbilical cord are from the cord blood and/or the Wharton's jelly. Inother embodiments, the cells isolated from the placenta tissue are fromthe membrane sheets (amnion and/or chorion), the villi, and/or theblood.

C. Methods to Induce Differentiation of Stem Cells

Disclosed herein are methods of inducing differentiation of stem cellsinto differentiated cell types, the method comprising culturing the stemcells in the presence of a cell-derived extracellular matrix (ECM) in adifferentiation media, wherein the cell-derived ECM is derived in vitrofrom cells isolated from amniotic fluid, i.e. the amniotic fluidcell-derived ECM of the invention. In some embodiments, the cell-derivedECM comprises laminin, collagen alpha-1 (XVIII), basementmembrane-specific heparan sulfate proteoglycan core protein, agrin,vimentin, and collagen alpha-2 (IV), and/or isoforms thereof. In someembodiments, the isoform of collagen alpha-1 (XVIII) is isoform 2. Insome embodiments, the isoform of agrin is isoform 6. In someembodiments, the cell-derived ECM further comprises fibronectin and/oran isoform thereof. In some embodiments, the cell-derived ECM does notcontain decorin, perlecan, or collagen (III). In some embodiments, thecells isolated from amniotic fluid comprise stem cells. In someembodiments, the cell-derived ECM is decellularized. The stem cells canbe pluripotent stem cells (PSCs), such as induced PSCs (iPSC) orembryonic stem cells (ES). The stem cells can be mesenchymal stem cells(MSCs). MSCs can be obtained from the following non-limiting sources:bone marrow, umbilical cord tissue, e.g. Wharton's jelly, umbilical cordblood, adipose tissue, and amniotic fluid. In some embodiments, the MSCsare obtained from bone marrow. In some embodiments, the differentiatedcell types can be adipocytes, osteoblasts, chondrocytes, or myocytes.

Cell culture techniques for differentiation of stem cells intodifferentiated cell types are known in the art and can be followed.Various commercially available differentiation media are suitable foruse and can be specific for a particular desired differentiated celltype. For example, an adipogenic differentiation media can comprise DMEMcontaining FBS, IBMX, dexamethasone, insulin, and/or indomethacin.Another example is an osteoblast differentiation media which can be agrowth media supplemented with dexamethasone and/orL-ascorbate-2-phosphate.

The methods of inducing differentiation of stem cells described supraalso apply to the use of culturing stem cells in the presence of otherperinatal cell-derived ECMs in differentiation media. In some aspects,disclosed is a method of inducing differentiation of stem cells intodifferentiated cell types, the method comprising culturing the stemcells in the presence of a cell-derived extracellular matrix (ECM) in adifferentiation media, wherein the cell-derived ECM is derived in vitrofrom cells isolated from an umbilical cord or placenta tissue. In someembodiments, the cells isolated from the umbilical cord are from thecord blood and/or the Wharton's jelly. In other embodiments, the cellsisolated from the placenta tissue are from the membrane sheets (amnionand/or chorion), the villi, and/or the blood.

EXAMPLES

The following examples are included to demonstrate certain non-limitingaspects of the invention. It should be appreciated by those of skill inthe art that the techniques disclosed in the examples that followrepresent techniques discovered by the applicants to function well inthe practice of the invention. However, those of skill in the artshould, in light of the present disclosure, appreciate that many changescan be made in the specific embodiments that are disclosed and stillobtain a like or similar result without departing from the spirit andscope of the invention.

Example 1—Production of an Amniotic Fluid Cell-Derived ECM

Four amniotic fluid cell-derived ECMs (Matrix A, Matrix B, Matrix C, andMatrix D) were made using the following procedure: cells asepticallyisolated from amniotic fluid collected from full term birth (>37 weeksgestational age) from 4 donors were seeded onto fibronectin coatedtissue-culture treated flasks and cultured in Complete Media at 37° C.,5% CO₂ and 90% RH in an incubator. The Complete Media was alpha MinimumEssential Media (aMEM) plus 2 mM L-Glutamine plus antibiotic-antimycoticplus 15% Fetal Bovine Serum.

At day 3-4, one-half of the complete medium was aspirated from theflasks and replaced with one-half of new Complete Media. The flasks wereplaced back into the incubator at the same conditions as stated above.

At day 7-8, the Complete Media was aspirated from the culture flasks andwas replenished with Inducing Media. The flasks were placed back intothe incubator at the same conditions as stated above. The Inducing Mediawas Complete Media plus 50 mM L-Ascorbic Acid.

At day 10-11, the Inducing Media was aspirated from the culture flasksand the ECM which had formed inside the flasks was washed one time withphosphate buffered saline (PBS). Then the PBS was aspirated from theflasks. An Extraction Buffer was added to the flasks and incubated for7-10 minutes at RT to decellularize each ECM, then the Extraction Bufferwas aspirated from the flasks. The Extraction Buffer was PBS containing0.5% (v/v) TRITON-X100 and 20 mM ammonium hydroxide (NH₄OH).

Each of the decellularized ECMs in the flasks was washed three timeswith PBS followed by one wash with sterile water and then the sterilewater was aspirated from the flasks. The four decellularized ECMs in theflasks were allowed to dry at RT and then stored at 4° C.

A photomicrograph of a Brightfield Image of an amniotic fluidcell-derived ECM (Matrix B) is shown in FIG. 1 at 100× power using a 10×objective lens. An atomic force photomicrograph of 3 representative40×40 um sections of the amniotic fluid cell-derived ECM (Matrix B) anda bone marrow cell-derived ECM showing topography, adhesion, andstiffness is shown in FIG. 2. The bone marrow- and amnioticfluid-cell-derived ECMs are structurally and physically distinct.Quantification of adhesion and stiffness (elastic modulus) of bonemarrow- and amniotic fluid-cell-derived ECMs show bone marrow ECM is10-fold stiffer, and 3-fold less adhesive, relative to amniotic fluidECM as shown in the scatter plots in FIG. 3a (Adhesion) and FIG. 3b(Stiffness) where BM=bone marrow cell-derive ECM, AD=amniotic fluidcell-derived ECM (Matrix B). Each point represents an independent pointof measurement.

Example 2—Composition of Amniotic Fluid Cell-Derived ECM

The composition of the each of the amniotic fluid cell-derived ECMsproduced in Example 1 was determined by mass spectrometry. Thecomponents with their spectral count and molecular weight are listed inTable 2.

TABLE 2 Amniotic Fluid Cell-Derived ECM Components Total Spectra CountProtein MW Matrix A Matrix B Matrix C Matrix D Isoform 7 of FibronectinOS = Homo sapiens OX = 9606 269 kDa  817 13965 1143 794 GN = FN1 Isoform3 of Fibronectin OS = Homo sapiens OX = 9606 259 kDa  807 13836 1150 790GN = FN1 Fibronectin OS = Homo sapiens OX = 9606 GN = FN1 PE = 1 263kDa  802 13851 1138 781 SV = 4 Isoform 14 of Fibronectin OS = Homosapiens OX = 9606 249 kDa  770 12625 1103 763 GN = FN1 Isoform 10 ofFibronectin OS = Homo sapiens OX = 9606 240 kDa  758 12506 1082 746 GN =FN1 Myosin-9 OS = Homo sapiens OX = 9606 GN = MYH9 227 kDa  564 3923 295340 PE = 1 SV = 4 SWISS-PROT:P60712 (Bos taurus) Actin, cytoplasmic 1 42kDa 293 2496 450 395 Vimentin OS = Homo sapiens OX = 9606 GN = VIM PE =1 54 kDa 263 1179 178 338 SV = 4 Neuroblast differentiation-associatedprotein AHNAK 629 kDa  250 803 17 184 OS = Homo sapiens OX = 9606 GN =AHNAK PE = 1 SV = 2 Histone H2B type 1-D OS = Homo sapiens OX = 9606 14kDa 212 1121 176 187 GN = HIST1H2BD PE = 1 SV = 2 Isoform 2 of Filamin-AOS = Homo sapiens OX = 9606 280 kDa  212 342 12 143 GN = FLNA Basementmembrane-specific heparan sulfate 469 kDa  202 0 402 338 proteoglycancore protein OS = Homo sapiens OX = 9606 GN = HSPG2 PE = 1 SV = 4Isoform 4 of Plectin OS = Homo sapiens OX = 9606 516 kDa  185 337 10 83GN = PLEC SWISS-PROT:P02769 (Bos taurus) Bovine serum 69 kDa 122 112 5372 albumin precursor Tubulin beta chain OS = Homo sapiens OX = 9606 50kDa 117 0 36 92 GN = TUBB PE = 1 SV = 2 Spectrin alpha chain,non-erythrocytic 1 OS = Homo 285 kDa  107 342 10 78 sapiens OX = 9606 GN= SPTAN1 PE = 1 SV = 3 Tubulin beta-4B chain OS = Homo sapiens OX = 960650 kDa 107 0 34 84 GN = TUBB4B PE = 1 SV = 1 Isoform 3 of Spectrin alphachain, non-erythrocytic 1 282 kDa  106 345 0 77 OS = Homo sapiens GN =SPTAN1 Histone H4 OS = Homo sapiens OX = 9606 11 kDa 100 1257 91 96 GN =HIST1H4A PE = 1 SV = 2 Tubulin beta-4A chain OS = Homo sapiens OX = 960650 kDa 99 0 31 76 GN = TUBB4A PE = 1 SV = 2 Protein-glutaminegamma-glutamyltransferase 2 77 kDa 94 188 73 25 OS = Homo sapiens OX =9606 GN = TGM2 PE = 1 SV = 2 SWISS-PROT:P00761|TRYP_PIG Trypsin - Susscrofa 24 kDa 94 362 121 96 (Pig). Tubulin alpha-1B chain OS = Homosapiens OX = 9606 50 kDa 93 295 37 86 GN = TUBA1B PE = 1 SV = 1 Isoform2 of Clathrin heavy chain 1 OS = Homo sapiens 188 kDa  92 97 1 15 OX =9606 GN = CLTC Tubulin beta-2A chain OS = Homo sapiens OX = 9606 50 kDa92 0 32 73 GN = TUBB2A PE = 1 SV = 1 Elongation factor 1-alpha 1 OS =Homo sapiens OX = 9606 50 kDa 89 132 14 70 GN = EEF1A1 PE = 1 SV = 1Isoform 2 of Tubulin alpha-1A chain OS = Homo sapiens 46 kDa 88 305 3482 OX = 9606 GN = TUBA1A Talin-1 OS = Homo sapiens OX = 9606 GN = TLN1PE = 1 270 kDa  88 109 4 41 SV = 3 Myosin-10 OS = Homo sapiens GN =MYH10 PE = 1 SV = 3 229 kDa  84 417 56 46 Spectrin beta chain,non-erythrocytic 1 OS = Homo 275 kDa  84 260 7 61 sapiens OX = 9606 GN =SPTBN1 PE = 1 SV = 2 Pyruvate kinase PKM OS = Homo sapiens OX = 9606 58kDa 83 73 21 46 GN = PKM PE = 1 SV = 4 Tubulin alpha-1C chain OS = Homosapiens OX = 9606 50 kDa 83 0 0 78 GN = TUBA1C PE = 1 SV = 1 Major vaultprotein OS = Homo sapiens OX = 9606 99 kDa 82 360 68 108 GN = MVP PE = 1SV = 4 Actin, aortic smooth muscle OS = Homo sapiens 42 kDa 78 0 126 77OX = 9606 GN = ACTA2 PE = 1 SV = 1 Actin, gamma-enteric smooth muscle OS= Homo sapiens 42 kDa 76 313 122 73 OX = 9606 GN = ACTG2 PE = 1 SV = 1Alpha-actinin-4 OS = Homo sapiens OX = 9606 105 kDa  75 199 11 76 GN =ACTN4 PE = 1 SV = 2 Isoform 8 of Filamin-B OS = Homo sapiens OX = 9606282 kDa  75 123 2 94 GN = FLNB Tubulin alpha-4A chain OS = Homo sapiensOX = 9606 50 kDa 73 262 0 65 GN = TUBA4A PE = 1 SV = 1Glyceraldehyde-3-phosphate dehydrogenase OS = Homo 36 kDa 71 145 24 66sapiens OX = 9606 GN = GAPDH PE = 1 SV = 3 Cytoplasmic dynein 1 heavychain 1 OS = Homo sapiens 532 kDa  65 49 0 5 OX = 9606 GN = DYNC1H1 PE =1 SV = 5 Histone H2A.J OS = Homo sapiens OX = 9606 GN = H2AFJ 14 kDa 65216 62 79 PE = 1 SV = 1 Serpin H1 OS = Homo sapiens OX = 9606 GN =SERPINH1 46 kDa 64 731 128 97 PE = 1 SV = 2 Histone H2A type 2-C OS =Homo sapiens OX = 9606 14 kDa 63 0 61 82 GN = HIST2H2AC PE = 1 SV = 4Alpha-actinin-1 OS = Homo sapiens GN = ACTN1 PE = 1 103 kDa  62 217 1977 SV = 2 Histone H2AX OS = Homo sapiens OX = 9606 15 kDa 61 0 69 75 GN= H2AFX PE = 1 SV = 2 Tubulin beta-3 chain OS = Homo sapiens OX = 960650 kDa 58 194 28 53 GN = TUBB3 PE = 1 SV = 2 Endoplasmic reticulumchaperone BiP OS = Homo 72 kDa 57 0 35 46 sapiens OX = 9606 GN = HSPA5PE = 1 SV = 2 Myosin regulatory light chain 12A OS = Homo sapiens 20 kDa57 0 244 153 OX = 9606 GN = MYL12A PE = 1 SV = 1 Myosin regulatory lightchain 12B OS = Homo sapiens 20 kDa 57 0 240 149 OX = 9606 GN = MYL12B PE= 1 SV = 2 Elongation factor 2 OS = Homo sapiens OX = 9606 95 kDa 53 723 45 GN = EEF2 PE = 1 SV = 4 Filamin-C OS = Homo sapiens OX = 9606 GN =FLNC 291 kDa  53 62 3 63 PE = 1 SV = 3 Histone H3.1 OS = Homo sapiens OX= 9606 15 kDa 53 0 24 33 GN = HIST1H3A PE = 1 SV = 2 60 kDa heat shockprotein, mitochondrial OS = Homo 61 kDa 52 58 14 58 sapiens OX = 9606 GN= HSPD1 PE = 1 SV = 2 Tubulin beta-6 chain OS = Homo sapiens OX = 960650 kDa 52 0 23 52 GN = TUBB6 PE = 1 SV = 1 Isoform 4 of Collagenalpha-1(XII) chain OS = Homo 325 kDa  51 35 36 27 sapiens OX = 9606 GN =COL12A1 Nucleophosmin OS = Homo sapiens OX = 9606 33 kDa 48 103 15 43 GN= NPM1 PE = 1 SV = 2 Prelamin-A/C OS = Homo sapiens OX = 9606 GN = LMNA74 kDa 48 149 20 39 PE = 1 SV = 1 Heat shock protein HSP 90-beta OS =Homo sapiens 83 kDa 47 0 3 31 OX = 9606 GN = HSP90AB1 PE = 1 SV = 4 RasGTPase-activating-like protein IQGAP1 OS = Homo 189 kDa  47 0 11 36sapiens OX = 9606 GN = IQGAP1 PE = 1 SV = 1 Heat shock cognate 71 kDaprotein OS = Homo sapiens 71 kDa 46 130 28 56 OX = 9606 GN = HSPA8 PE =1 SV = 1 Annexin A2 OS = Homo sapiens OX = 9606 GN = ANXA2 39 kDa 43 1115 40 PE = 1 SV = 2 Isoform 2 of Collagen alpha-1(XVIII) chain OS = Homo154 kDa  42 575 77 61 sapiens OX = 9606 GN = COL18A1 Myosin regulatorylight polypeptide 9 OS = Homo sapiens 20 kDa 42 380 131 81 OX = 9606 GN= MYL9 PE = 1 SV = 4 Isoform 6 of Agrin OS = Homo sapiens OX = 9606 215kDa  41 168 42 70 GN = AGRN Histone H3.2 OS = Homo sapiens OX = 9606 15kDa 40 0 17 25 GN = HIST2H3A PE = 1 SV = 3 Isoform 1 of Core histonemacro-H2A.1 OS = Homo 39 kDa 40 450 43 49 sapiens OX = 9606 GN = H2AFYATP synthase subunit beta, mitochondrial OS = Homo 57 kDa 39 94 14 45sapiens OX = 9606 GN = ATP5F1B PE = 1 SV = 3 T-complex protein 1 subunitalpha OS = Homo sapiens 60 kDa 39 0 25 36 OX = 9606 GN = TCP1 PE = 1 SV= 1 Isoform 2 of Heat shock protein HSP 90-alpha 98 kDa 38 80 1 20 OS =Homo sapiens GN = HSP90AA1 Transitional endoplasmic reticulum ATPase OS= Homo 89 kDa 38 86 1 37 sapiens OX = 9606 GN = VCP PE = 1 SV = 4TREMBL:Q3KNV1; Q96GE1 Tax_Id = 9606 51 kDa 38 334 58 133 Gene_Symbol =KRT7 keratin 7 (Bos taurus) similar to alpha-2-macroglobulin isoform 1164 kDa  37 14 9 13 Heterogeneous nuclear ribonucleoprotein U OS = Homo91 kDa 35 64 12 29 sapiens OX = 9606 GN = HNRNPU PE = 1 SV = 6 HistoneH3.3 OS = Homo sapiens OX = 9606 GN = H3F3A 15 kDa 35 0 17 43 PE = 1 SV= 2 Microtubule-associated protein 4 OS = Homo sapiens 121 kDa  35 73 826 OX = 9606 GN = MAP4 PE = 1 SV = 3 Beta-actin-like protein 2 OS = Homosapiens OX = 9606 42 kDa 34 147 42 31 GN = ACTBL2 PE = 1 SV = 2 Keratin,type I cytoskeletal 10 OS = Homo sapiens 59 kDa 34 464 32 37 OX = 9606GN = KRT10 PE = 1 SV = 6 Ribosome-binding protein 1 OS = Homo sapiens152 kDa  34 80 17 22 OX = 9606 GN = RRBP1 PE = 1 SV = 5Cytoskeleton-associated protein 4 OS = Homo sapiens 66 kDa 33 139 30 35OX = 9606 GN = CKAP4 PE = 1 SV = 2 DNA-dependent protein kinasecatalytic subunit 469 kDa  33 10 0 5 OS = Homo sapiens OX = 9606 GN =PRKDC PE = 1 SV = 3 Adenylyl cyclase-associated protein 1 OS = Homosapiens 52 kDa 32 33 2 37 OX = 9606 GN = CAP1 PE = 1 SV = 5 Keratin,type I cytoskeletal 9 OS = Homo sapiens 62 kDa 31 643 61 50 OX = 9606 GN= KRT9 PE = 1 SV = 3 Laminin subunit alpha-5 OS = Homo sapiens OX = 9606400 kDa  31 50 7 13 GN = LAMA5 PE = 1 SV = 8 (Bos taurus) similar tofibulin-1 C isoform 1 77 kDa 30 40 18 71 60S ribosomal protein L4 OS =Homo sapiens OX = 9606 48 kDa 30 75 26 25 GN = RPL4 PE = 1 SV = 5Alpha-enolase OS = Homo sapiens OX = 9606 GN = ENO1 47 kDa 30 61 3 26 PE= 1 SV = 2 ATP synthase subunit alpha, mitochondrial OS = Homo 60 kDa 3052 12 34 sapiens OX = 9606 GN = ATP5F1A PE = 1 SV = 1 Heterogeneousnuclear ribonucleoprotein M OS = Homo 78 kDa 30 114 12 30 sapiens OX =9606 GN = HNRNPM PE = 1 SV = 3 Histone H2A.V OS = Homo sapiens OX = 960614 kDa 30 131 23 34 GN = H2AFV PE = 1 SV = 3 Lysyl oxidase homolog 2 OS= Homo sapiens OX = 9606 87 kDa 30 320 94 52 GN = LOXL2 PE = 1 SV = 1MICOS complex subunit MIC60 OS = Homo sapiens 84 kDa 30 98 20 36 OX =9606 GN = IMMT PE = 1 SV = 1 SWISS-PROT:P12763 (Bos taurus) Alpha-2-HS-38 kDa 30 42 18 19 glycoprotein precursor Endoplasmin OS = Homo sapiensOX = 9606 92 kDa 29 71 3 22 GN = HSP90B1 PE = 1 SV = 1 TREMBL:Q0IIK2(Bos taurus) Transferrin 78 kDa 29 15 2 16 Laminin subunit beta-1 OS =Homo sapiens OX = 9606 198 kDa  28 30 4 11 GN = LAMB1 PE = 1 SV = 2T-complex protein 1 subunit beta OS = Homo sapiens 57 kDa 27 117 19 31OX = 9606 GN = CCT2 PE = 1 SV = 4 T-complex protein 1 subunit delta OS =Homo sapiens 58 kDa 27 73 19 26 OX = 9606 GN = CCT4 PE = 1 SV = 4Heterogeneous nuclear ribonucleoprotein K OS = Homo 51 kDa 26 28 3 26sapiens OX = 9606 GN = HNRNPK PE = 1 SV = 1 Bifunctionalglutamate/proline--tRNA ligase OS = Homo 171 kDa  25 20 0 1 sapiens OX =9606 GN = EPRS PE = 1 SV = 5 Isoform 1 of Vinculin OS = Homo sapiens OX= 9606 117 kDa  25 25 1 26 GN = VCL Isoform 2 of MICOS complex subunitMIC60 OS = Homo 83 kDa 25 99 17 34 sapiens OX = 9606 GN = IMMT T-complexprotein 1 subunit theta OS = Homo sapiens 60 kDa 25 100 21 30 OX = 9606GN = CCT8 PE = 1 SV = 4 Isoform LCRMP-4 of Dihydropyrimidinase-related74 kDa 24 11 4 40 protein 3 OS = Homo sapiens OX = 9606 GN = DPYSL3Thrombospondin-1 OS = Homo sapiens OX = 9606 129 kDa  24 103 3 1 GN =THBS1 PE = 1 SV = 2 Isoform Short of 14-3-3 protein beta/alpha OS = Homo28 kDa  23 18 2 14 sapiens OX = 9606 GN = YWHAB Isoform Smooth muscle ofMyosin light polypeptide 6 17 kDa 23 204 37 28 OS = Homo sapiens OX =9606 GN = MYL6 Keratin, type I cytoskeletal 18 OS = Homo sapiens 48 kDa23 376 99 120 OX = 9606 GN = KRT18 PE = 1 SV = 2 Keratin, type IIcytoskeletal 8 OS = Homo sapiens 54 kDa 23 1404 107 155 OX = 9606 GN =KRT8 PE = 1 SV = 7 Lamin-B1 OS = Homo sapiens OX = 9606 GN = LMNB1 66kDa 23 67 7 21 PE = 1 SV = 2 L-lactate dehydrogenase A chain OS = Homosapiens 37 kDa 23 18 2 10 GN = LDHA PE = 1 SV = 2 Profilin-1 OS = Homosapiens OX = 9606 GN = PFN1 PE = 1 15 kDa 23 0 6 17 SV = 2 Proteindisulfide-isomerase OS = Homo sapiens OX = 9606 57 kDa 23 0 6 19 GN =P4HB PE = 1 SV = 3 T-complex protein 1 subunit gamma OS = Homo sapiens61 kDa 23 72 17 26 GN = CCT3 PE = 1 SV = 4 40S ribosomal protein S15 OS= Homo sapiens OX = 9606 17 kDa 22 0 26 38 GN = RPS15 PE = 1 SV = 2ATP-citrate synthase OS = Homo sapiens OX = 9606 121 kDa  22 20 2 18 GN= ACLY PE = 1 SV = 3 Isoform 2 of Transgelin-2 OS = Homo sapiens OX =9606 24 kDa 22 47 1 20 GN = TAGLN2 Versican core protein OS = Homosapiens OX = 9606 373 kDa  22 188 33 27 GN = VCAN PE = 1 SV = 3 60Sacidic ribosomal protein P0 OS = Homo sapiens 34 kDa 21 56 14 19 OX =9606 GN = RPLPO PE = 1 SV = 1 Histone H1.5 OS = Homo sapiens OX = 960623 kDa 21 53 11 20 GN = HIST1H1B PE = 1 SV = 3 Importin subunit beta-1OS = Homo sapiens OX = 9606 97 kDa 21 19 1 6 GN = KPNB1 PE = 1 SV = 2Isoform 2 of Fructose-bisphosphate aldolase A 45 kDa 21 37 0 21 OS =Homo sapiens OX = 9606 GN = ALDOA Microtubule-associated protein 1B OS =Homo sapiens 271 kDa  21 104 0 22 OX = 9606 GN = MAP1B PE = 1 SV = 2TREMBL:Q3SX09 (Bos taurus) similar to HBG protein 22 kDa 21 26 11 95′-nucleotidase OS = Homo sapiens OX = 9606 GN = NT5E 63 kDa 20 129 2117 PE = 1 SV = 1 60S ribosomal protein L3 OS = Homo sapiens OX = 9606 46kDa 20 65 15 24 GN = RPL3 PE = 1 SV = 2 60S ribosomal protein L6 OS =Homo sapiens OX = 9606 33 kDa 20 0 19 25 GN = RPL6 PE = 1 SV = 3ATP-dependent 6-phosphofructokinase, platelet type 86 kDa 20 9 0 18 OS =Homo sapiens OX = 9606 GN = PFKP PE = 1 SV = 2 Collagen alpha-1(I) chainOS = Homo sapiens OX = 9606 139 kDa  20 41 43 27 GN = COL1A1 PE = 1 SV =5 T-complex protein 1 subunit eta OS = Homo sapiens 59 kDa 20 72 15 22OX = 9606 GN = CCT7 PE = 1 SV = 2 Trifunctional enzyme subunit alpha,mitochondrial 83 kDa 20 78 16 13 OS = Homo sapiens OX = 9606 GN = HADHAPE = 1 SV = 2 40S ribosomal protein S7 OS = Homo sapiens OX = 9606 22kDa 19 104 25 28 GN = RPS7 PE = 1 SV = 1 60S ribosomal protein L9 OS =Homo sapiens OX = 9606 22 kDa 19 0 14 21 GN = RPL9 PE = 1 SV = 1Actin-related protein 3 OS = Homo sapiens OX = 9606 47 kDa 19 0 16 18 GN= ACTR3 PE = 1 SV = 3 Annexin A5 OS = Homo sapiens OX = 9606 GN = ANXA536 kDa 19 0 0 15 PE = 1 SV = 2 Calpain-2 catalytic subunit OS = Homosapiens OX = 9606 80 kDa 19 4 0 8 GN = CAPN2 PE = 1 SV = 6 Heterogeneousnuclear ribonucleoprotein A1 OS = Homo 39 kDa 19 55 10 23 sapiens OX =9606 GN = HNRNPA1 PE = 1 SV = 5 Integrin beta-1 OS = Homo sapiens OX =9606 GN = ITGB1 88 kDa 19 16 4 18 PE = 1 SV = 2 Interleukinenhancer-binding factor 2 OS = Homo sapiens 43 kDa 19 0 4 15 OX = 9606GN = ILF2 PE = 1 SV = 2 Isoform 2 of Nidogen-2 OS = Homo sapiens OX =9606 141 kDa  19 55 0 3 GN = NID2 Matrin-3 OS = Homo sapiens OX = 9606GN = MATR3 95 kDa 19 46 5 20 PE = 1 SV = 2 T-complex protein 1 subunitzeta OS = Homo sapiens 58 kDa 19 64 23 28 OX = 9606 GN = CCT6A PE = 1 SV= 3 40S ribosomal protein S2 OS = Homo sapiens OX = 9606 31 kDa 18 0 1417 GN = RPS2 PE = 1 SV = 2 40S ribosomal protein S3 OS = Homo sapiens OX= 9606 27 kDa 18 82 19 20 GN = RPS3 PE = 1 SV = 2 Collagen alpha-2(IV)chain OS = Homo sapiens OX = 9606 168 kDa  18 209 44 33 GN = COL4A2 PE =1 SV = 4 Epiplakin OS = Homo sapiens OX = 9606 GN = EPPK1 556 kDa  18 221 39 PE = 1 SV = 3 Heat shock 70 kDa protein 1A OS = Homo sapiens 70 kDa18 31 0 23 OX = 9606 GN = HSPA1A PE = 1 SV = 1 Heterogeneous nuclearribonucleoproteins C1/C2 34 kDa 18 52 6 22 OS = Homo sapiens OX = 9606GN = HNRNPC PE = 1 SV = 4 Isoform 2 of Gelsolin OS = Homo sapiens OX =9606 81 kDa 18 87 36 24 GN = GSN Isoform 5 of Septin-9 OS = Homo sapiensOX = 9606 65 kDa 18 42 1 16 GN = SEPT9 Laminin subunit gamma-1 OS = Homosapiens OX = 9606 178 kDa  18 48 0 6 GN = LAMC1 PE = 1 SV = 3 NucleolinOS = Homo sapiens OX = 9606 GN = NCL PE = 1 77 kDa 18 58 8 16 SV = 3Voltage-dependent anion-selective channel protein 1 31 kDa 18 50 4 19 OS= Homo sapiens OX = 9606 GN = VDAC1 PE = 1 SV = 2 X-ray repaircross-complementing protein 6 OS = Homo 70 kDa 18 33 2 14 sapiens OX =9606 GN = XRCC6 PE = 1 SV = 2 Actin-related protein 2 OS = Homo sapiensOX = 9606 45 kDa 17 43 17 28 GN = ACTR2 PE = 1 SV = 1 Heat shock proteinbeta-1 OS = Homo sapiens OX = 9606 23 kDa 17 0 9 35 GN = HSPB1 PE = 1 SV= 2 Histone H1.4 OS = Homo sapiens OX = 9606 22 kDa 17 14 10 13 GN =HIST1H1E PE = 1 SV = 2 Isoform 2 of AP-2 complex subunit beta OS = Homo106 kDa  17 33 1 15 sapiens OX = 9606 GN = AP2B1 Isoform 2 of CalnexinOS = Homo sapiens OX = 9606 72 kDa 17 21 1 19 GN = CANX Isoform 2 ofDolichyl-diphosphooligosaccharide--protein 68 kDa 17 35 8 25glycosyltransferase subunit 2 OS = Homo sapiens OX = 9606 GN = RPN2Moesin OS = Homo sapiens OX = 9606 GN = MSN PE = 1 68 kDa 17 61 3 15 SV= 3 Protein disulfide-isomerase A3 OS = Homo sapiens 57 kDa 17 26 0 14OX = 9606 GN = PDIA3 PE = 1 SV = 4 Stress-70 protein, mitochondrial OS =Homo sapiens 74 kDa 17 33 0 10 OX = 9606 GN = HSPA9 PE = 1 SV = 2SWISS-PROT:P34955 (Bos taurus) Alpha-1- 46 kDa 17 66 19 16antiproteinase precursor T-complex protein 1 subunit epsilon OS = Homosapiens 60 kDa 17 110 18 22 OX = 9606 GN = CCT5 PE = 1 SV = 1 14-3-3protein zeta/delta OS = Homo sapiens OX = 9606 28 kDa 16 35 4 19 GN =YWHAZ PE = 1 SV = 1 40S ribosomal protein S4, X isoform OS = Homosapiens 30 kDa 16 77 20 20 OX = 9606 GN = RPS4X PE = 1 SV = 2 60Sribosomal protein L10 OS = Homo sapiens OX = 9606 25 kDa 16 27 7 12 GN =RPL10 PE = 1 SV = 4 Ezrin OS = Homo sapiens OX = 9606 GN = EZR PE = 1 69kDa 16 43 4 23 SV = 4 High mobility group protein HMG-I/HMG-Y OS = Homo12 kDa 16 52 7 11 sapiens OX = 9606 GN = HMGA1 PE = 1 SV = 3 Hypoxiaup-regulated protein 1 OS = Homo sapiens 111 kDa  16 37 0 18 OX = 9606GN = HYOU1 PE = 1 SV = 1 Isoform 2 of Ubiquitin-like modifier-activatingenzyme 1 114 kDa  16 21 0 5 OS = Homo sapiens OX = 9606 GN = UBA1Isoform 3 of Heterogeneous nuclear ribonucleoprotein Q 63 kDa 37 5 16 20OS = Homo sapiens OX = 9606 GN = SYNCRIP Nidogen-1 OS = Homo sapiens OX= 9606 GN = NID1 136 kDa  16 56 1 7 PE = 1 SV = 3 14-3-3 protein epsilonOS = Homo sapiens OX = 9606 29 kDa 15 26 3 16 GN = YWHAE PE = 1 SV = 140S ribosomal protein S5 OS = Homo sapiens OX = 9606 23 kDa 15 0 10 12GN = RPS5 PE = 1 SV = 4 60S ribosomal protein L5 OS = Homo sapiens OX =9606 34 kDa 15 0 16 16 GN = RPL5 PE = 1 SV = 3 60S ribosomal protein L7OS = Homo sapiens OX = 9606 29 kDa 15 61 14 14 GN = RPL7 PE = 1 SV = 1Caprin-1 OS = Homo sapiens OX = 9606 GN = CAPRIN1 78 kDa 15 18 0 14 PE =1 SV = 2 Catenin alpha-1 OS = Homo sapiens OX = 9606 100 kDa  15 13 0 12GN = CTNNA1 PE = 1 SV = 1 Eukaryotic initiation factor 4A-I OS = Homosapiens 46 kDa 15 28 2 8 OX = 9606 GN = EIF4A1 PE = 1 SV = 1Heterogeneous nuclear ribonucleoproteins A2/B1 37 kDa 15 58 6 20 OS =Homo sapiens OX = 9606 GN = HNRNPA2B1 PE = 1 SV = 2 Isoform 2 ofCoatomer subunit alpha OS = Homo sapiens 139 kDa  15 8 0 0 OX = 9606 GN= COPA Isoform 2 of Protein disulfide-isomerase A6 OS = Homo 54 kDa 1523 0 15 sapiens OX = 9606 GN = PDIA6 Isoform 3 of Septin-2 OS = Homosapiens OX = 9606 43 kDa 15 55 6 16 GN = SEPT2 Staphylococcal nucleasedomain-containing protein 1 102 kDa  15 33 0 6 OS = Homo sapiens OX =9606 GN = SND1 PE = 1 SV = 1 Triosephosphate isomerase OS = Homo sapiensOX = 9606 31 kDa 15 15 0 11 GN = TPI1 PE = 1 SV = 3 UDP-glucose6-dehydrogenase OS = Homo sapiens 55 kDa 15 24 2 15 OX = 9606 GN = UGDHPE = 1 SV = 1 ADP/ATP translocase 2 OS = Homo sapiens OX = 9606 33 kDa14 27 9 17 GN = SLC25A5 PE = 1 SV = 7 ATP-dependent RNA helicase A OS =Homo sapiens 141 kDa  14 25 1 9 OX = 9606 GN = DHX9 PE = 1 SV = 4Cofilin-1 OS = Homo sapiens OX = 9606 GN = CFL1 PE = 1 19 kDa 14 0 4 11SV = 3 eIF-2-alpha kinase activator GCN1 OS = Homo sapiens 293 kDa  14 00 6 OX = 9606 GN = GCN1 PE = 1 SV = 6 Isoform 2 of Kinectin OS = Homosapiens OX = 9606 150 kDa  14 62 1 8 GN = KTN1 Isoform 2 of SpliceosomeRNA helicase DDX39B 51 kDa 14 25 1 12 OS = Homo sapiens OX = 9606 GN =DDX39B Isoleucine--tRNA ligase, cytoplasmic OS = Homo sapiens 145 kDa 14 0 0 0 OX = 9606 GN = IARS PE = 1 SV = 2 Keratin, type II cytoskeletal2 epidermal OS = Homo 65 kDa 14 123 28 21 sapiens OX = 9606 GN = KRT2 PE= 1 SV = 2 Ribonuclease inhibitor OS = Homo sapiens OX = 9606 50 kDa 140 1 8 GN = RNH1 PE = 1 SV = 2 SWISS-PROT:P02070 (Bos taurus) Hemoglobinsubunit 16 kDa 14 0 0 0 beta SWISS-PROT:Q9DCV7 Tax_Id = 10090 51 kDa 140 14 18 Gene_Symbol = Krt7 Keratin, type II cytoskeletal 7Tubulointerstitial nephritis antigen-like OS = Homo 52 kDa 14 173 78 38sapiens OX = 9606 GN = TINAGL1 PE = 1 SV = 1 26S proteasome non-ATPaseregulatory subunit 2 100 kDa  13 18 0 10 OS = Homo sapiens OX = 9606 GN= PSMD2 PE = 1 SV = 3 40S ribosomal protein S8 OS = Homo sapiens OX =9606 24 kDa 13 0 9 15 GN = RPS8 PE = 1 SV = 2 60S ribosomal protein L23OS = Homo sapiens OX = 9606 15 kDa 13 0 14 10 GN = RPL23 PE = 1 SV = 160S ribosomal protein L7a OS = Homo sapiens OX = 9606 30 kDa 13 68 12 14GN = RPL7A PE = 1 SV = 2 ADP/ATP translocase 3 OS = Homo sapiens OX =9606 33 kDa 13 0 8 18 GN = SLC25A6 PE = 1 SV = 4 Arginine--tRNA ligase,cytoplasmic OS = Homo sapiens 75 kDa 13 33 0 5 OX = 9606 GN = RARS PE =1 SV = 2 Calpain small subunit 1 OS = Homo sapiens OX = 9606 28 kDa 13 00 22 GN = CAPNS1 PE = 1 SV = 1 Glycine--tRNA ligase OS = Homo sapiens OX= 9606 83 kDa 13 0 1 6 GN = GARS PE = 1 SV = 3 Isoform 2 of Extendedsynaptotagmin-1 OS = Homo 124 kDa  13 21 1 17 sapiens OX = 9606 GN =ESYT1 Isoform 2 of Nuclear mitotic apparatus protein 1 237 kDa  13 18 02 OS = Homo sapiens OX = 9606 GN = NUMA1 Isoform 2 of Tropomyosin betachain OS = Homo sapiens 33 kDa 13 122 33 24 OX = 9606 GN = TPM2 Isoform7 of Interleukin enhancer-binding factor 3 96 kDa 13 31 0 13 OS = Homosapiens GN = ILF3 Isoform Beta-1 of DNA topoisomerase 2-beta OS = Homo183 kDa  13 97 3 3 sapiens OX = 9606 GN = TOP2B Lamin-B2 OS = Homosapiens OX = 9606 GN = LMNB2 70 kDa 13 53 8 19 PE = 1 SV = 4 L-lactatedehydrogenase B chain OS = Homo sapiens 37 kDa 13 0 0 6 OX = 9606 GN =LDHB PE = 1 SV = 2 Nucleoprotein TPR OS = Homo sapiens OX = 9606 267kDa  13 15 1 3 GN = TPR PE = 1 SV = 3 Receptor of activated protein Ckinase 1 OS = Homo 35 kDa 13 0 1 12 sapiens OX = 9606 GN = RACK1 PE = 1SV = 3 Serine protease HTRA1 OS = Homo sapiens OX = 9606 51 kDa 13 0 151 GN = HTRA1 PE = 1 SV = 1 Serine/threonine-protein phosphatase 2A 65kDa 65 kDa 13 0 0 10 regulatory subunit A alpha isoform OS = Homosapiens OX = 9606 GN = PPP2R1A PE = 1 SV = 4 X-ray repaircross-complementing protein 5 OS = Homo 83 kDa 13 0 2 15 sapiens OX =9606 GN = XRCC5 PE = 1 SV = 3 14-3-3 protein theta OS = Homo sapiens OX= 9606 28 kDa 12 0 2 12 GN = YWHAQ PE = 1 SV = 1 40S ribosomal proteinS12 OS = Homo sapiens OX = 9606 15 kDa 12 0 1 9 GN = RPS12 PE = 1 SV = 3Cytoplasmic dynein 1 light intermediate chain 2 54 kDa 12 12 3 19 OS =Homo sapiens OX = 9606 GN = DYNC1LI2 PE = 1 SV = 1 GlutathioneS-transferase P OS = Homo sapiens OX = 9606 23 kDa 12 0 2 13 GN = GSTP1PE = 1 SV = 2 Isoform 2 of Septin-7 OS = Homo sapiens OX = 9606 51 kDa12 31 0 12 GN = SEPT7 Isoform 3 of Unconventional myosin-Ic OS = Homo120 kDa  12 64 9 8 sapiens OX = 9606 GN = MYO1C Peptidyl-prolylcis-trans isomerase A OS = Homo sapiens 18 kDa 12 36 3 11 OX = 9606 GN =PPIA PE = 1 SV = 2 Polyadenylate-binding protein 1 OS = Homo sapiens 71kDa 12 22 0 15 OX = 9606 GN = PABPC1 PE = 1 SV = 2 Rho-relatedGTP-binding protein RhoC OS = Homo 22 kDa 12 0 5 12 sapiens OX = 9606 GN= RHOC PE = 1 SV = 1 Ribosomal L1 domain-containing protein 1 OS = Homo55 kDa 12 18 8 12 sapiens OX = 9606 GN = RSL1D1 PE = 1 SV = 3 TransgelinOS = Homo sapiens OX = 9606 GN = TAGLN 23 kDa 12 41 10 23 PE = 1 SV = 414-3-3 protein gamma OS = Homo sapiens OX = 9606 28 kDa 11 0 1 14 GN =YWHAG PE = 1 SV = 2 26S proteasome regulatory subunit 6A OS = Homo 47kDa 11 0 0 17 sapiens OX = 9606 GN = PSMC3 PE = 1 SV = 1 40S ribosomalprotein S17 OS = Homo sapiens OX = 9606 16 kDa 11 67 20 16 GN = RPS17 PE= 1 SV = 2 60S ribosomal protein L10a OS = Homo sapiens 25 kDa 11 0 1117 OX = 9606 GN = RPL10A PE = 1 SV = 2 60S ribosomal protein L12 OS =Homo sapiens OX = 9606 18 kDa 11 36 10 9 GN = RPL12 PE = 1 SV = 1ADP-ribosylation factor 4 OS = Homo sapiens OX = 9606 21 kDa 11 0 8 14GN = ARF4 PE = 1 SV = 3 Annexin A6 OS = Homo sapiens OX = 9606 GN =ANXA6 76 kDa 11 15 0 9 PE = 1 SV = 3 ATP synthase subunit O,mitochondrial OS = Homo 23 kDa 11 0 5 10 sapiens OX = 9606 GN = ATP5POPE = 1 SV = 1 Core histone macro-H2A.2 OS = Homo sapiens OX = 9606 40kDa 11 226 28 21 GN = H2AFY2 PE = 1 SV = 3Dolichyl-diphosphooligosaccharide--protein 69 kDa 11 43 7 18glycosyltransferase subunit 1 OS = Homo sapiens OX = 9606 GN = RPN1 PE =1 SV = 1 Elongation factor 1-gamma OS = Homo sapiens OX = 9606 50 kDa 1129 0 8 GN = EEF1G PE = 1 SV = 3 Guanine nucleotide-binding protein G(i)subunit alpha-2 40 kDa 11 45 6 8 OS = Homo sapiens OX = 9606 GN = GNAI2PE = 1 SV = 3 Heterogeneous nuclear ribonucleoprotein R OS = Homo 71 kDa11 17 4 12 sapiens OX = 9606 GN = HNRNPR PE = 1 SV = 1 Isoform 2 ofProbable ATP-dependent RNA helicase 72 kDa 11 17 0 9 DDX17 OS = Homosapiens OX = 9606 GN = DDX17 Isoform 3 of Tropomyosin alpha-1 chain OS =Homo 33 kDa 11 181 46 37 sapiens OX = 9606 GN = TPM1 Isoform 4 ofCaldesmon OS = Homo sapiens OX = 9606 63 kDa 11 51 10 32 GN = CALD1Leucine--tRNA ligase, cytoplasmic OS = Homo sapiens 134 kDa  11 3 0 0 GN= LARS PE = 1 SV = 2 Phosphoglycerate kinase 1 OS = Homo sapiens OX =9606 45 kDa 11 10 0 7 GN = PGK1 PE = 1 SV = 3 Polypyrimidinetract-binding protein 1 OS = Homo 57 kDa 11 33 1 20 sapiens OX = 9606 GN= PTBP1 PE = 1 SV = 1 Rab GDP dissociation inhibitor beta OS = Homosapiens 51 kDa 11 17 0 10 OX = 9606 GN = GDI2 PE = 1 SV = 2 Reticulon-4OS = Homo sapiens GN = RTN4 PE = 1 SV = 2 130 kDa  11 15 0 9 Serinehydroxymethyltransferase, mitochondrial 56 kDa 11 6 0 3 OS = Homosapiens GN = SHMT2 PE = 1 SV = 3 Serine/threonine-protein phosphatase PP1-alpha catalytic 38 kDa 11 23 3 10 subunit OS = Homo sapiens OX = 9606GN = PPP1CA PE = 1 SV = 1 Splicing factor, proline- and glutamine-richOS = Homo 76 kDa 11 43 5 18 sapiens OX = 9606 GN = SFPQ PE = 1 SV = 2SWISS-PROT:Q3MHN5 (Bos taurus) Vitamin D- 53 kDa 11 15 2 4 bindingprotein precursor Tenascin OS = Homo sapiens GN = TNC PE = 1 SV = 3 241kDa  11 409 60 72 Threonine--tRNA ligase, cytoplasmic OS = Homo sapiens83 kDa 11 10 0 3 OX = 9606 GN = TARS PE = 1 SV = 3 Tropomyosin alpha-4chain OS = Homo sapiens OX = 9606 29 kDa 11 89 21 20 GN = TPM4 PE = 1 SV= 3 14-3-3 protein eta OS = Homo sapiens OX = 9606 28 kDa 10 0 1 10 GN =YWHAH PE = 1 SV = 4 26S proteasome regulatory subunit 6B OS = Homosapiens 47 kDa 10 13 3 10 OX = 9606 GN = PSMC4 PE = 1 SV = 2 60Sribosomal protein L18 OS = Homo sapiens 19 kDa 10 26 7 9 GN = RPL18 PE =1 SV = 1 Actin-related protein 2/3 complex subunit 2 OS = Homo 34 kDa 1053 14 11 sapiens OX = 9606 GN = ARPC2 PE = 1 SV = 1 A-kinase anchorprotein 12 OS = Homo sapiens OX = 9606 191 kDa  10 46 0 5 GN = AKAP12 PE= 1 SV = 4 Asparagine--tRNA ligase, cytoplasmic OS = Homo 63 kDa 10 10 01 sapiens OX = 9606 GN = NARS PE = 1 SV = 1 Aspartate--tRNA ligase,cytoplasmic OS = Homo sapiens 57 kDa 10 22 3 9 OX = 9606 GN = DARS PE =1 SV = 2 Dolichyl-diphosphooligosaccharide--protein 51 kDa 10 13 6 17glycosyltransferase 48 kDa subunit OS = Homo sapiens OX = 9606 GN =DDOST PE = 1 SV = 4 Erlin-2 OS = Homo sapiens OX = 9606 GN = ERLIN2 PE =1 38 kDa 10 39 4 11 SV = 1 Fatty acid synthase OS = Homo sapiens OX =9606 273 kDa  10 0 0 2 GN = FASN PE = 1 SV = 3 Heterogeneous nuclearribonucleoprotein A3 OS = Homo 40 kDa 10 25 7 8 sapiens OX = 9606 GN =HNRNPA3 PE = 1 SV = 2 Isoform 3 of Exportin-2 OS = Homo sapiens OX =9606 108 kDa  10 2 2 3 GN = CSE1L Isoform B of AP-1 complex subunitbeta-1 OS = Homo 104 kDa  10 0 0 11 sapiens OX = 9606 GN = AP1B1Leucine-rich PPR motif-containing protein, 158 kDa  10 5 0 0mitochondrial OS = Homo sapiens OX = 9606 GN = LRPPRC PE = 1 SV = 3Non-POU domain-containing octamer-binding protein 54 kDa 10 44 3 20 OS =Homo sapiens OX = 9606 GN = NONO PE = 1 SV = 4 Nucleolar protein 56 OS =Homo sapiens OX = 9606 66 kDa 10 18 1 2 GN = NOP56 PE = 1 SV = 4Peroxidasin homolog OS = Homo sapiens OX = 9606 165 kDa  10 146 36 28 GN= PXDN PE = 1 SV = 2 Stomatin-like protein 2, mitochondrial OS = Homosapiens 39 kDa 10 18 3 10 OX = 9606 GN = STOML2 PE = 1 SV = 1SWISS-PROT:P01966 (Bos taurus) Hemoglobin subunit 15 kDa 10 34 12 10alpha SWISS-PROT:Q3SZ57 (Bos taurus) Alpha-fetoprotein 69 kDa 10 11 6 7precursor Transforming protein RhoA OS = Homo sapiens 22 kDa 10 0 4 10OX = 9606 GN = RHOA PE = 1 SV = 1 40S ribosomal protein SA OS = Homosapiens OX = 9606 33 kDa 9 0 1 10 GN = RPSA PE = 1 SV = 1 60S ribosomalprotein L13 OS = Homo sapiens OX = 9606 24 kDa 9 32 12 12 GN = RPL13 PE= 1 SV = 4 60S ribosomal protein L8 OS = Homo sapiens OX = 9606 28 kDa 90 7 9 GN = RPL8 PE = 1 SV = 2 Aldehyde dehydrogenase X, mitochondrial OS= Homo 57 kDa 9 14 4 11 sapiens OX = 9606 GN = ALDH1B1 PE = 1 SV = 3ATP-dependent RNA helicase DDX3X OS = Homo 73 kDa 9 18 5 11 sapiens OX =9606 GN = DDX3X PE = 1 SV = 3 Calreticulin OS = Homo sapiens OX = 9606GN = CALR 48 kDa 9 44 4 11 PE = 1 SV = 1 D-3-phosphoglyceratedehydrogenase OS = Homo sapiens 57 kDa 9 0 0 2 OX = 9606 GN = PHGDH PE =1 SV = 4 F-actin-capping protein subunit alpha-1 OS = Homo 33 kDa 9 0 1815 sapiens OX = 9606 GN = CAPZA1 PE = 1 SV = 3 Galectin-1 OS = Homosapiens OX = 9606 GN = LGALS1 15 kDa 9 0 3 6 PE = 1 SV = 2 GTP-bindingnuclear protein Ran OS = Homo sapiens 24 kDa 9 0 6 11 OX = 9606 GN = RANPE = 1 SV = 3 Heterochromatin protein 1-binding protein 3 OS = Homo 61kDa 9 24 4 7 sapiens OX = 9606 GN = HP1BP3 PE = 1 SV = 1 Isoform 1 ofVoltage-dependent anion-selective channel 33 kDa 9 19 1 5 protein 2 OS =Homo sapiens OX = 9606 GN = VDAC2 Isoform 2 of Eukaryotic translationinitiation factor 3 163 kDa  9 10 0 11 subunit A OS = Homo sapiens OX =9606 GN = EIF3A Isoform 2 of Glutamine--tRNA ligase OS = Homo sapiens 87kDa 9 16 0 4 OX = 9606 GN = QARS Isoform 2 of Tropomyosin alpha-3 chainOS = Homo 29 kDa 9 99 16 17 sapiens OX = 9606 GN = TPM3 Isoform 3 ofProtein AHNAK2 OS = Homo sapiens 606 kDa  9 0 0 1 OX = 9606 GN = AHNAK2Isoform D of Eukaryotic translation initiation factor 4 159 kDa  9 7 0 0gamma 1 OS = Homo sapiens OX = 9606 GN = EIF4G1 KH domain-containing,RNA-binding, signal 48 kDa 9 24 5 9 transduction-associated protein 1 OS= Homo sapiens OX = 9606 GN = KHDRBS1 PE = 1 SV = 1 Methionine--tRNAligase, cytoplasmic OS = Homo 101 kDa  9 1 0 0 sapiens OX = 9606 GN =MARS PE = 1 SV = 2 Myeloid-associated differentiation marker OS = Homo35 kDa 9 0 16 12 sapiens OX = 9606 GN = MYADM PE = 1 SV = 2 Neutralalpha-glucosidase AB OS = Homo sapiens 107 kDa  9 21 0 13 OX = 9606 GN =GANAB PE = 1 SV = 3 Peroxiredoxin-5, mitochondrial OS = Homo sapiens 22kDa 9 6 0 5 OX = 9606 GN = PRDX5 PE = 1 SV = 4 Serine--tRNA ligase,cytoplasmic OS = Homo sapiens 59 kDa 9 0 0 9 OX = 9606 GN = SARS PE = 1SV = 3 Tryptophan--tRNA ligase, cytoplasmic OS = Homo 53 kDa 9 6 1 7sapiens OX = 9606 GN = WARS PE = 1 SV = 2 Vacuolar proteinsorting-associated protein 35 OS = Homo 92 kDa 9 5 0 4 sapiens OX = 9606GN = VPS35 PE = 1 SV = 2 26S proteasome non-ATPase regulatory subunit 361 kDa 8 21 0 6 OS = Homo sapiens OX = 9606 GN = PSMD3 PE = 1 SV = 2 40Sribosomal protein S16 OS = Homo sapiens OX = 9606 16 kDa 8 0 9 7 GN =RPS16 PE = 1 SV = 2 40S ribosomal protein S6 OS = Homo sapiens OX = 960629 kDa 8 0 5 11 GN = RPS6 PE = 1 SV = 1 40S ribosomal protein S9 OS =Homo sapiens OX = 9606 23 kDa 8 54 11 10 GN = RPS9 PE = 1 SV = 3 ADP/ATPtranslocase 1 OS = Homo sapiens OX = 9606 33 kDa 8 0 0 12 GN = SLC25A4PE = 1 SV = 4 Annexin A1 OS = Homo sapiens OX = 9606 GN = ANXA1 39 kDa 80 0 8 PE = 1 SV = 2 ATP-dependent RNA helicase DDX1 OS = Homo sapiens 82kDa 8 15 2 10 OX = 9606 GN = DDX1 PE = 1 SV = 2 Coatomer subunit gamma-1OS = Homo sapiens 98 kDa 8 9 1 6 OX = 9606 GN = COPG1 PE = 1 SV = 1Elongation factor Tu, mitochondrial OS = Homo sapiens 50 kDa 8 0 0 0 OX= 9606 GN = TUFM PE = 1 SV = 2 Erythrocyte band 7 integral membraneprotein OS = Homo 32 kDa 8 6 2 3 sapiens OX = 9606 GN = STOM PE = 1 SV =3 Eukaryotic translation initiation factor 2 subunit 3 51 kDa 8 0 3 12OS = Homo sapiens OX = 9606 GN = EIF2S3 PE = 1 SV = 3 Flotillin-1 OS =Homo sapiens OX = 9606 GN = FLOT1 47 kDa 8 25 1 4 PE = 1 SV = 3Heterogeneous nuclear ribonucleoprotein L OS = Homo 64 kDa 8 11 0 5sapiens OX = 9606 GN = HNRNPL PE = 1 SV = 2 Isoform 2 of Ankycorbin OS =Homo sapiens OX = 9606 110 kDa  8 38 2 5 GN = RAI14 Isoform 2 ofBifunctional purine biosynthesis protein 65 kDa 8 5 0 4 PURH OS = Homosapiens OX = 9606 GN = ATIC Isoform 2 of Eukaryotic translationinitiation factor 5A-1 20 kDa 8 14 1 7 OS = Homo sapiens OX = 9606 GN =EIF5A Isoform 2 of Golgi apparatus protein 1 OS = Homo 137 kDa  8 4 9 1sapiens OX = 9606 GN = GLG1 Isoform 2 of Poly(rC)-binding protein 2 OS =Homo 39 kDa 8 4 0 4 sapiens OX = 9606 GN = PCBP2 Isoform 3 ofHeterogeneous nuclear ribonucleoprotein 33 kDa 8 20 6 11 D0 OS = Homosapiens OX = 9606 GN = HNRNPD Isoform B of Phosphate carrier protein,mitochondrial 40 kDa 8 7 5 8 OS = Homo sapiens OX = 9606 GN = SLC25A3Kinesin-1 heavy chain OS = Homo sapiens OX = 9606 110 kDa  8 5 0 3 GN =KIF5B PE = 1 SV = 1 Malate dehydrogenase, mitochondrial OS = Homosapiens 36 kDa 8 17 0 13 OX = 9606 GN = MDH2 PE = 1 SV = 3 Mitochondrialcarrier homolog 2 OS = Homo sapiens 33 kDa 8 0 2 4 OX = 9606 GN = MTCH2PE = 1 SV = 1 Pre-mRNA-processing factor 19 OS = Homo sapiens 55 kDa 8 00 14 OX = 9606 GN = PRPF19 PE = 1 SV = 1 Prohibitin OS = Homo sapiens OX= 9606 GN = PHB PE = 1 30 kDa 8 14 2 15 SV = 1 Protein transport proteinSec23A OS = Homo sapiens 86 kDa 8 9 1 10 OX = 9606 GN = SEC23A PE = 1 SV= 2 Protein transport protein Sec61 subunit alpha isoform 1 52 kDa 8 2 15 OS = Homo sapiens OX = 9606 GN = SEC61A1 PE = 1 SV = 2 Septin-11 OS =Homo sapiens OX = 9606 GN = SEPT11 49 kDa 8 27 0 9 PE = 1 SV = 3 Smallnuclear ribonucleoprotein Sm D1 OS = Homo 13 kDa 8 0 6 7 sapiens OX =9606 GN = SNRPD1 PE = 1 SV = 1 SWISS-PROT:P15497 (Bos taurus)Apolipoprotein A-I 30 kDa 8 21 6 4 precursor THO complex subunit 4 OS =Homo sapiens OX = 9606 27 kDa 8 37 7 11 GN = ALYREF PE = 1 SV = 3TREMBL:Q3ZBS7 (Bos taurus) Vitronectin 54 kDa 8 26 12 11 Ubiquitin-40Sribosomal protein S27a OS = Homo sapiens 18 kDa 8 0 13 10 OX = 9606 GN =RPS27A PE = 1 SV = 2 26S proteasome non-ATPase regulatory subunit 12 53kDa 7 6 0 2 OS = Homo sapiens OX = 9606 GN = PSMD12 PE = 1 SV = 3 26Sproteasome regulatory subunit 8 OS = Homo sapiens 46 kDa 7 6 0 12 OX =9606 GN = PSMC5 PE = 1 SV = 1 40S ribosomal protein S14 OS = Homosapiens OX = 9606 16 kDa 7 40 13 9 GN = RPS14 PE = 1 SV = 3 40Sribosomal protein S19 OS = Homo sapiens OX = 9606 16 kDa 7 0 12 15 GN =RPS19 PE = 1 SV = 2 40S ribosomal protein S23 OS = Homo sapiens OX =9606 16 kDa 7 0 4 12 GN = RPS23 PE = 1 SV = 3 60S ribosomal protein L30OS = Homo sapiens OX = 9606 13 kDa 7 0 8 8 GN = RPL30 PE = 1 SV = 2ADP-ribosylation factor 3 OS = Homo sapiens OX = 9606 21 kDa 7 11 7 7 GN= ARF3 PE = 1 SV = 2 Aminoacyl tRNA synthase complex-interacting 35 kDa7 0 0 6 multifunctional protein 2 OS = Homo sapiens OX = 9606 GN = AIMP2PE = 1 SV = 2 CAD protein OS = Homo sapiens OX = 9606 GN = CAD 243 kDa 7 0 0 0 PE = 1 SV = 3 Catenin beta-1 OS = Homo sapiens OX = 9606 85 kDa7 3 0 6 GN = CTNNB1 PE = 1 SV = 1 Cell division control protein 42homolog OS = Homo 21 kDa 7 0 2 11 sapiens OX = 9606 GN = CDC42 PE = 1 SV= 2 Chloride intracellular channel protein 1 OS = Homo 27 kDa 7 0 0 5sapiens OX = 9606 GN = CLIC1 PE = 1 SV = 4 Coatomer subunit beta OS =Homo sapiens OX = 9606 107 kDa  7 5 0 1 GN = COPB1 PE = 1 SV = 3Eukaryotic initiation factor 4A-III OS = Homo sapiens 47 kDa 7 20 4 8 OX= 9606 GN = EIF4A3 PE = 1 SV = 4 Heterogeneous nuclear ribonucleoproteinH OS = Homo 51 kDa 7 23 1 9 sapiens OX = 9606 GN = HNRNPH1 PE = 1 SV = 1Importin-5 OS = Homo sapiens OX = 9606 GN = IPO5 124 kDa  7 5 0 3 PE = 1SV = 4 Inhibitor of nuclear factor kappa-B kinase-interacting 39 kDa 7 76 7 protein OS = Homo sapiens OX = 9606 GN = IKBIP PE = 1 SV = 1 Isoform2 of 26S proteasome non-ATPase regulatory 48 kDa 7 12 0 5 subunit 11 OS= Homo sapiens OX = 9606 GN = PSMD11 Isoform 2 of F-actin-cappingprotein subunit beta 31 kDa 7 46 10 5 OS = Homo sapiens OX = 9606 GN =CAPZB Isoform 2 of Heterogeneous nuclear ribonucleoprotein D- 34 kDa 7 80 7 like OS = Homo sapiens OX = 9606 GN = HNRNPDL Isoform 2 of Integrinalpha-3 OS = Homo sapiens 119 kDa  7 2 0 5 OX = 9606 GN = ITGA3 Isoform2 of Nucleolar RNA helicase 2 OS = Homo 80 kDa 7 16 2 7 sapiens OX =9606 GN = DDX21 Isoform 2 of PDZ and LIM domain protein 7 OS = Homo 47kDa 7 13 0 5 sapiens OX = 9606 GN = PDLIM7 Isoform 2 of Sperminesynthase OS = Homo sapiens 35 kDa 7 2 0 6 OX = 9606 GN = SMS Isoform 2of Transketolase OS = Homo sapiens OX = 9606 69 kDa 7 29 0 9 GN = TKTIsoform 3 of 116 kDa U5 small nuclear ribonucleoprotein 108 kDa  7 6 0 9component OS = Homo sapiens OX = 9606 GN = EFTUD2 Isoform 3 of 60Sribosomal protein L17 OS = Homo 26 kDa 7 54 5 8 sapiens OX = 9606 GN =RPL17 Isoform 3 of Integrin alpha-V OS = Homo sapiens 111 kDa  7 22 2 12OX = 9606 GN = ITGAV Isoform SV3 of Supervillin OS = Homo sapiens OX =9606 245 kDa  7 46 0 1 GN = SVIL NADH dehydrogenase [ubiquinone] 1 alphasubcomplex 43 kDa 7 12 1 7 subunit 9, mitochondrial OS = Homo sapiens OX= 9606 GN = NDUFA9 PE = 1 SV = 2 Peroxiredoxin-1 OS = Homo sapiens OX =9606 22 kDa 7 0 0 7 GN = PRDX1 PE = 1 SV = 1 Poly(rC)-binding protein 1OS = Homo sapiens OX = 9606 37 kDa 7 0 0 2 GN = PCBP1 PE = 1 SV = 2Pre-mRNA-processing-splicing factor 8 OS = Homo 274 kDa  7 6 1 3 sapiensOX = 9606 GN = PRPF8 PE = 1 SV = 2 Probable ATP-dependent RNA helicaseDDX5 69 kDa 7 25 7 19 OS = Homo sapiens OX = 9606 GN = DDX5 PE = 1 SV =1 Prohibitin-2 OS = Homo sapiens OX = 9606 GN = PHB2 33 kDa 7 25 5 12 PE= 1 SV = 2 Protein disulfide-isomerase A4 OS = Homo sapiens 73 kDa 7 4 04 OX = 9606 GN = PDIA4 PE = 1 SV = 2 Ras GTPase-activatingprotein-binding protein 1 52 kDa 7 19 2 8 OS = Homo sapiens OX = 9606 GN= G3BP1 PE = 1 SV = 1 Ras-related protein Rab-1B OS = Homo sapiens OX =9606 22 kDa 7 0 0 8 GN = RAB1B PE = 1 SV = 1 RNA-binding motif protein,X chromosome OS = Homo 42 kDa 7 22 5 13 sapiens OX = 9606 GN = RBMX PE =1 SV = 3 Splicing factor 3B subunit 1 OS = Homo sapiens 146 kDa  7 16 13 OX = 9606 GN = SF3B1 PE = 1 SV = 3 SWISS-PROT:P02535-1 Tax_Id = 1009058 kDa 7 39 7 0 Gene_Symbol = Krt10 Isoform 1 of Keratin, type Icytoskeletal 10 SWISS-PROT:P08730-1 Tax_Id = 10090 48 kDa 7 451 17 31Gene_Symbol = Krt13 Isoform 1 of Keratin, type I cytoskeletal 13SWISS-PROT:Q6IFZ6 Tax_Id = 10090 61 kDa 7 32 10 5 Gene_Symbol = Krt77Keratin, type II cytoskeletal 1b Talin-2 OS = Homo sapiens OX = 9606 GN= TLN2 PE = 1 272 kDa  7 0 0 1 SV = 4 Transcription intermediary factor1-beta OS = Homo 89 kDa 7 5 0 0 sapiens OX = 9606 GN = TRIM28 PE = 1 SV= 5 TREMBL:Q9TRI1 (Bos taurus) similar to inter-alpha- 106 kDa  7 57 7 8trypsin inhibitor heavy chain2 U5 small nuclear ribonucleoprotein 200kDa helicase 245 kDa  7 1 0 0 OS = Homo sapiens OX = 9606 GN = SNRNP200PE = 1 SV = 2 Valine--tRNA ligase OS = Homo sapiens OX = 9606 140 kDa  78 0 4 GN = VARS PE = 1 SV = 4 26S proteasome regulatory subunit 4 OS =Homo sapiens 49 kDa 6 25 1 6 OX = 9606 GN = PSMC1 PE = 1 SV = 1 40Sribosomal protein S26 OS = Homo sapiens OX = 9606 13 kDa 6 0 6 6 GN =RPS26 PE = 1 SV = 3 60S ribosomal protein L24 OS = Homo sapiens OX =9606 18 kDa 6 0 5 6 GN = RPL24 PE = 1 SV = 1 60S ribosomal protein L27aOS = Homo sapiens 17 kDa 6 0 6 4 OX = 9606 GN = RPL27A PE = 1 SV = 2Alpha-centractin OS = Homo sapiens OX = 9606 43 kDa 6 26 3 11 GN =ACTR1A PE = 1 SV = 1 ATP-dependent RNA helicase DDX18 OS = Homo 75 kDa 65 3 8 sapiens OX = 9606 GN = DDX18 PE = 1 SV = 2 Collagen alpha-1(IV)chain OS = Homo sapiens OX = 9606 161 kDa  6 69 21 7 GN = COL4A1 PE = 1SV = 4 Collagen alpha-1(VIII) chain OS = Homo sapiens 73 kDa 6 0 29 22OX = 9606 GN = COL8A1 PE = 1 SV = 2 Cullin-associated NEDD8-dissociatedprotein 1 136 kDa  6 0 0 3 OS = Homo sapiens GN = CAND1 PE = 1 SV = 2Dihydrolipoyllysine-residue succinyltransferase 49 kDa 6 17 0 8component of 2-oxoglutarate dehydrogenase complex, mitochondrial OS =Homo sapiens OX = 9606 GN = DLST PE = 1 SV = 4Dihydropyrimidinase-related protein 2 OS = Homo 62 kDa 6 3 0 5 sapiensOX = 9606 GN = DPYSL2 PE = 1 SV = 1 Eukaryotic translation initiationfactor 3 subunit M 43 kDa 6 11 0 5 OS = Homo sapiens OX = 9606 GN =EIF3M PE = 1 SV = 1 Exportin-1 OS = Homo sapiens OX = 9606 GN = XPO1 123kDa  6 0 0 0 PE = 1 SV = 1 F-actin-capping protein subunit alpha-2 OS =Homo 33 kDa 6 33 8 9 sapiens OX = 9606 GN = CAPZA2 PE = 1 SV = 3Glycogen phosphorylase, brain form OS = Homo sapiens 97 kDa 6 0 0 1 OX =9606 GN = PYGB PE = 1 SV = 5 Importin-7 OS = Homo sapiens OX = 9606 GN =IPO7 120 kDa  6 0 0 0 PE = 1 SV = 1 Isoform 2 of 6-phosphogluconatedehydrogenase, 52 kDa 6 4 0 3 decarboxylating OS = Homo sapiens OX =9606 GN = PGD Isoform 2 of Coatomer subunit beta′ OS = Homo sapiens 99kDa 6 10 0 4 OX = 9606 GN = COPB2 Isoform 2 of Coronin-1C OS = Homosapiens OX = 9606 54 kDa 6 36 5 7 GN = CORO1C Isoform 2 of Elongationfactor 1-delta OS = Homo sapiens 71 kDa 6 32 3 4 OX = 9606 GN = EEF1DIsoform 2 of Inverted formin-2 OS = Homo sapiens 135 kDa  6 4 0 1 OX =9606 GN = INF2 Isoform 2 of Programmed cell death 6-interacting protein97 kDa 6 3 0 4 OS = Homo sapiens OX = 9606 GN = PDCD6IP Isoform 2 ofSurfeit locus protein 4 OS = Homo sapiens 18 kDa 6 10 3 7 OX = 9606 GN =SURF4 Isoform 3 of Plasminogen activator inhibitor 1 RNA- 43 kDa 6 11 67 binding protein OS = Homo sapiens OX = 9606 GN = SERBP1 Isoform 5 ofPhosphatidylinositol-binding clathrin 70 kDa 6 5 0 7 assembly protein OS= Homo sapiens GN = PICALM Junction plakoglobin OS = Homo sapiens OX =9606 82 kDa 6 38 0 6 GN = JUP PE = 1 SV = 3 Lamina-associatedpolypeptide 2, isoforms beta/gamma 51 kDa 6 26 7 7 OS = Homo sapiens OX= 9606 GN = TMPO PE = 1 SV = 2 Leucine-rich repeat-containing protein 59OS = Homo 35 kDa 6 0 7 10 sapiens OX = 9606 GN = LRRC59 PE = 1 SV = 1Multifunctional protein ADE2 OS = Homo sapiens 47 kDa 6 3 0 2 OX = 9606GN = PAICS PE = 1 SV = 3 Nicotinamide N-methyltransferase OS = Homosapiens 30 kDa 6 0 0 1 OX = 9606 GN = NNMT PE = 1 SV = 1 Poly[ADP-ribose] polymerase 4 OS = Homo sapiens 193 kDa  6 19 0 5 OX = 9606GN = PARP4 PE = 1 SV = 3 Protein S100-A6 OS = Homo sapiens OX = 9606 10kDa 6 0 1 4 GN = S100A6 PE = 1 SV = 1 RuvB-like 2 OS = Homo sapiens OX =9606 GN = RUVBL2 51 kDa 6 19 0 5 PE = 1 SV = 3 Sarcoplasmic/endoplasmicreticulum calcium ATPase 2 115 kDa  6 6 0 0 OS = Homo sapiens GN =ATP2A2 PE = 1 SV = 1 Signal transducer and activator of transcription 1-87 kDa 6 15 1 5 alpha/beta OS = Homo sapiens OX = 9606 GN = STAT1 PE = 1SV = 2 Synaptic vesicle membrane protein VAT-1 homolog 42 kDa 6 6 0 7 OS= Homo sapiens OX = 9606 GN = VAT1 PE = 1 SV = 2 Ubiquitincarboxyl-terminal hydrolase OS = Homo sapiens 27 kDa 6 0 0 4 OX = 9606GN = UCHL1 PE = 1 SV = 1 Very-long-chain 3-oxoacyl-CoA reductase OS =Homo 34 kDa 6 0 2 6 sapiens OX = 9606 GN = HSD17B12 PE = 1 SV = 2 26Sproteasome non-ATPase regulatory subunit 13 43 kDa 5 9 0 4 OS = Homosapiens OX = 9606 GN = PSMD13 PE = 1 SV = 2 26S proteasome regulatorysubunit 10B OS = Homo 44 kDa 5 0 0 5 sapiens OX = 9606 GN = PSMC6 PE = 1SV = 1 40S ribosomal protein S10 OS = Homo sapiens OX = 9606 19 kDa 5 3813 12 GN = RPS10 PE = 1 SV = 1 40S ribosomal protein S11 OS = Homosapiens OX = 9606 18 kDa 5 26 3 7 GN = RPS11 PE = 1 SV = 3 60S ribosomalprotein L21 OS = Homo sapiens OX = 9606 19 kDa 5 34 8 7 GN = RPL21 PE =1 SV = 2 60S ribosomal protein L22 OS = Homo sapiens OX = 9606 15 kDa 50 6 8 GN = RPL22 PE = 1 SV = 2 60S ribosomal protein L23a (Fragment) OS= Homo 19 kDa 5 0 9 9 sapiens OX = 9606 GN = RPL23A PE = 1 SV = 1 60Sribosomal protein L27 OS = Homo sapiens OX = 9606 16 kDa 5 0 6 11 GN =RPL27 PE = 1 SV = 2 60S ribosomal protein L28 OS = Homo sapiens OX =9606 16 kDa 5 13 4 4 GN = RPL28 PE = 1 SV = 3 60S ribosomal protein L31OS = Homo sapiens OX = 9606 14 kDa 5 33 8 6 GN = RPL31 PE = 1 SV = 1 60Sribosomal protein L36 OS = Homo sapiens OX = 9606 12 kDa 5 0 7 7 GN =RPL36 PE = 1 SV = 3 Actin-related protein 2/3 complex subunit 3 OS =Homo 21 kDa 5 0 4 7 sapiens OX = 9606 GN = ARPC3 PE = 1 SV = 3Actin-related protein 2/3 complex subunit 4 OS = Homo 20 kDa 5 19 2 6sapiens OX = 9606 GN = ARPC4 PE = 1 SV = 3 Calpain-1 catalytic subunitOS = Homo sapiens OX = 9606 82 kDa 5 27 0 6 GN = CAPN1 PE = 1 SV = 1Calponin-2 OS = Homo sapiens OX = 9606 GN = CNN2 34 kDa 5 5 1 6 PE = 1SV = 4 Cathepsin D OS = Homo sapiens OX = 9606 GN = CTSD 45 kDa 5 0 0 3PE = 1 SV = 1 Cleavage and polyadenylation specificity factor subunit 526 kDa 5 0 0 6 OS = Homo sapiens OX = 9606 GN = NUDT21 PE = 1 SV = 1Coatomer subunit epsilon OS = Homo sapiens GN = COPE 34 kDa 5 14 2 8 PE= 1 SV = 3 Copine-3 OS = Homo sapiens OX = 9606 GN = CPNE3 60 kDa 5 0 01 PE = 1 SV = 1 Cytochrome c oxidase subunit 2 OS = Homo sapiens 26 kDa5 0 0 6 OX = 9606 GN = MT-CO2 PE = 1 SV = 1 DNA-(apurinic orapyrimidinic site) lyase OS = Homo 36 kDa 5 8 0 3 sapiens OX = 9606 GN =APEX1 PE = 1 SV = 2 Erlin-1 OS = Homo sapiens OX = 9606 GN = ERLIN1 PE =1 39 kDa 5 0 0 4 SV = 1 Eukaryotic translation elongation factor 1epsilon-1 20 kDa 5 7 2 7 OS = Homo sapiens GN = EEF1E1 PE = 1 SV = 1Eukaryotic translation initiation factor 2 subunit 1 36 kDa 5 9 1 7 OS =Homo sapiens OX = 9606 GN = EIF2S1 PE = 1 SV = 3 Eukaryotic translationinitiation factor 3 subunit H 42 kDa 5 0 0 3 OS = Homo sapiens OX = 9606GN = EIF3H PE = 1 SV = 1 Heat shock 70 kDa protein 4 OS = Homo sapiens94 kDa 5 4 0 1 OX = 9606 GN = HSPA4 PE = 1 SV = 4 High mobility groupprotein B1 OS = Homo sapiens 25 kDa 5 0 0 3 OX = 9606 GN = HMGB1 PE = 1SV = 3 Importin-9 OS = Homo sapiens OX = 9606 GN = IPO9 116 kDa  5 0 0 4PE = 1 SV = 3 Isoform 2 of 26S proteasome non-ATPase regulatory 102 kDa 5 10 0 3 subunit 1 OS = Homo sapiens OX = 9606 GN = PSMD1 Isoform 2 ofCalcium-binding mitochondrial carrier 74 kDa 5 10 0 9 protein Aralar2 OS= Homo sapiens OX = 9606 GN = SLC25A13 Isoform 2 of Collagen alpha-3(VI)chain OS = Homo 321 kDa  5 0 1 1 sapiens OX = 9606 GN = COL6A3 Isoform 2of Eukaryotic translation initiation factor 3 99 kDa 5 5 0 6 subunit BOS = Homo sapiens OX = 9606 GN = EIF3B Isoform 2 of Glucose-6-phosphateisomerase OS = Homo 64 kDa 5 4 0 2 sapiens OX = 9606 GN = GPI Isoform 2of HLA class I histocompatibility antigen, A- 41 kDa 5 3 0 3 11 alphachain OS = Homo sapiens OX = 9606 GN = HLA-A Isoform 2 of Myb-bindingprotein 1A OS = Homo sapiens 149 kDa  5 1 0 0 OX = 9606 GN = MYBBP1AIsoform 2 of Serine/arginine-rich splicing factor 2 24 kDa 5 2 0 2 OS =Homo sapiens OX = 9606 GN = SRSF2 Isoform 2 of U1 small nuclearribonucleoprotein 70 kDa 51 kDa 5 0 0 5 OS = Homo sapiens OX = 9606 GN =SNRNP70 Isoform 3 of Glutaminase kidney isoform, mitochondrial 65 kDa 57 0 3 OS = Homo sapiens OX = 9606 GN = GLS Isoform 4 of AP-3 complexsubunit delta-1 OS = Homo 115 kDa  5 5 0 2 sapiens GN = AP3D1 Isoform 4of Protein phosphatase 1 regulatory subunit 109 kDa  5 10 3 7 12A OS =Homo sapiens OX = 9606 GN = PPP1R12A Isoform Short of Eukaryotictranslation initiation factor 25 kDa 5 5 1 4 4H OS = Homo sapiens OX =9606 GN = EIF4H Lon protease homolog, mitochondrial OS = Homo sapiens106 kDa  5 0 0 6 GN = LONP1 PE = 1 SV = 2 Mannosyl-oligosaccharideglucosidase OS = Homo 92 kDa 5 22 4 11 sapiens GN = MOGS PE = 1 SV = 5Prolyl 3-hydroxylase 1 OS = Homo sapiens OX = 9606 83 kDa 5 6 0 3 GN =P3H1 PE = 1 SV = 2 Proteasome subunit alpha type-4 OS = Homo sapiens 29kDa 5 0 0 7 OX = 9606 GN = PSMA4 PE = 1 SV = 1 Protein LYRIC OS = Homosapiens OX = 9606 64 kDa 5 10 6 9 GN = MTDH PE = 1 SV = 2Protein/nucleic acid deglycase DJ-1 OS = Homo sapiens 20 kDa 5 0 0 3 OX= 9606 GN = PARK7 PE = 1 SV = 2 Ras-related protein Rab-1A OS = Homosapiens OX = 9606 23 kDa 5 13 2 8 GN = RAB1A PE = 1 SV = 3 Ras-relatedprotein Rab-3B OS = Homo sapiens OX = 9606 25 kDa 5 0 2 7 GN = RAB3B PE= 1 SV = 2 Rho GDP-dissociation inhibitor 1 OS = Homo sapiens 23 kDa 5 00 4 OX = 9606 GN = ARHGDIA PE = 1 SV = 3 Signal recognition particlesubunit SRP72 OS = Homo 75 kDa 5 8 1 8 sapiens OX = 9606 GN = SRP72 PE =1 SV = 3 Small nuclear ribonucleoprotein Sm D2 OS = Homo 14 kDa 5 11 0 5sapiens OX = 9606 GN = SNRPD2 PE = 1 SV = 1 Sorting and assemblymachinery component 50 homolog 52 kDa 5 19 4 12 OS = Homo sapiens OX =9606 GN = SAMM50 PE = 1 SV = 3 Src substrate cortactin OS = Homo sapiensOX = 9606 62 kDa 5 38 12 8 GN = CTTN PE = 1 SV = 2 SWI/SNF complexsubunit SMARCC1 OS = Homo 123 kDa  5 0 0 2 sapiens OX = 9606 GN =SMARCC1 PE = 1 SV = 3 Transcription factor BTF3 OS = Homo sapiens OX =9606 22 kDa 5 8 0 7 GN = BTF3 PE = 1 SV = 1 Translocon-associatedprotein subunit delta OS = Homo 19 kDa 5 0 2 4 sapiens OX = 9606 GN =SSR4 PE = 1 SV = 1 TREMBL:Q1RMK2 (Bos taurus) IGHM protein 65 kDa 5 0 00 WD repeat-containing protein 1 OS = Homo sapiens 66 kDa 5 11 0 5 OX =9606 GN = WDR1 PE = 1 SV = 4 Zyxin OS = Homo sapiens OX = 9606 GN = ZYXPE = 1 61 kDa 5 13 0 4 SV = 1 2-oxoglutarate dehydrogenase,mitochondrial OS = Homo 116 kDa  4 2 0 6 sapiens OX = 9606 GN = OGDH PE= 1 SV = 3 40S ribosomal protein S27-like OS = Homo sapiens  9 kDa 4 0 11 OX = 9606 GN = RPS27L PE = 1 SV = 3 Adenosylhomocysteinase OS = Homosapiens OX = 9606 48 kDa 4 4 0 2 GN = AHCY PE = 1 SV = 4ADP-ribosylation factor 6 OS = Homo sapiens OX = 9606 20 kDa 4 0 0 2 GN= ARF6 PE = 1 SV = 2 Alanine--tRNA ligase, cytoplasmic OS = Homo sapiens107 kDa  4 0 0 2 OX = 9606 GN = AARS PE = 1 SV = 2 ATPase family AAAdomain-containing protein 3A 71 kDa 4 22 5 9 OS = Homo sapiens OX = 9606GN = ATAD3A PE = 1 SV = 2 Coatomer subunit delta OS = Homo sapiens OX =9606 57 kDa 4 10 0 5 GN = ARCN1 PE = 1 SV = 1 Cold-inducible RNA-bindingprotein OS = Homo sapiens 19 kDa 4 3 0 4 GN = CIRBP PE = 1 SV = 1Copine-1 OS = Homo sapiens OX = 9606 GN = CPNE1 59 kDa 4 0 0 2 PE = 1 SV= 1 Cytochrome b-c1 complex subunit 2, mitochondrial 48 kDa 4 0 0 8 OS =Homo sapiens OX = 9606 GN = UQCRC2 PE = 1 SV = 3 DBH-like monooxygenaseprotein 1 OS = Homo sapiens 70 kDa 4 17 5 4 OX = 9606 GN = MOXD1 PE = 1SV = 1 EH domain-containing protein 1 OS = Homo sapiens 61 kDa 4 11 0 9OX = 9606 GN = EHD1 PE = 1 SV = 2 Eukaryotic translation initiationfactor 3 subunit C 105 kDa  4 4 0 5 OS = Homo sapiens OX = 9606 GN =EIF3C PE = 1 SV = 1 Eukaryotic translation initiation factor 4 gamma 2102 kDa  4 8 1 3 OS = Homo sapiens GN = EIF4G2 PE = 1 SV = 1 Eukaryotictranslation initiation factor 6 OS = Homo 27 kDa 4 10 2 5 sapiens OX =9606 GN = EIF6 PE = 1 SV = 1 Guanine nucleotide-binding proteinG(I)/G(S)/G(T) 37 kDa 4 12 3 4 subunit beta-1 OS = Homo sapiens OX =9606 GN = GNB1 PE = 1 SV = 3 Isoform 10 of Calpastatin OS = Homo sapiensOX = 9606 82 kDa 4 4 0 1 GN = CAST Isoform 2 of 40S ribosomal proteinS24 OS = Homo 15 kDa 4 46 10 8 sapiens OX = 9606 GN = RPS24 Isoform 2 ofChromodomain-helicase-DNA-binding 221 kDa  4 5 0 1 protein 4 OS = Homosapiens GN = CHD4 Isoform 2 of Eukaryotic translation initiation factor3 61 kDa 4 5 0 6 subunit L OS = Homo sapiens OX = 9606 GN = EIF3LIsoform 2 of Glucosidase 2 subunit beta OS = Homo 59 kDa 4 5 0 4 sapiensOX = 9606 GN = PRKCSH Isoform 2 of Hexokinase-1 OS = Homo sapiens OX =9606 102 kDa  4 3 0 1 GN = HK1 Isoform 2 of Isocitrate dehydrogenase[NADP], 45 kDa 4 19 1 4 mitochondrial OS = Homo sapiens OX = 9606 GN =IDH2 Isoform 2 of Nodal modulator 2 OS = Homo sapiens 134 kDa  4 4 0 3OX = 9606 GN = NOMO2 Isoform 2 of Plastin-3 OS = Homo sapiens OX = 960669 kDa 4 0 0 1 GN = PLS3 Isoform 2 of Proteasome subunit alpha type-3 OS= Homo 28 kDa 4 6 0 4 sapiens OX = 9606 GN = PSMA3 Isoform 2 of ProteinSET OS = Homo sapiens GN = SET 32 kDa 4 7 0 4 Isoform 2 of Splicingfactor U2AF 65 kDa subunit 53 kDa 4 1 0 5 OS = Homo sapiens OX = 9606 GN= U2AF2 Isoform 2 of SWI/SNF complex subunit SMARCC2 125 kDa  4 9 0 3 OS= Homo sapiens OX = 9606 GN = SMARCC2 Isoform 2 of Unconventionalmyosin-Ib OS = Homo 125 kDa  4 16 0 0 sapiens OX = 9606 GN = MYO1BIsoform 3 of Dynactin subunit 1 OS = Homo sapiens 137 kDa  4 8 0 2 OX =9606 GN = DCTN1 Isoform 3 of Heterogeneous nuclear ribonucleoprotein 31kDa 4 10 2 6 A/B OS = Homo sapiens GN = HNRNPAB Isoform 3 of MyoferlinOS = Homo sapiens OX = 9606 233 kDa  4 6 0 1 GN = MYOF Isoform 3 ofNucleolar and coiled-body phosphoprotein 1 74 kDa 4 7 1 3 OS = Homosapiens OX = 9606 GN = NOLC1 Isoform 4 of Inhibitor of nuclear factorkappa-B kinase- 43 kDa 4 11 2 5 interacting protein OS = Homo sapiens OX= 9606 GN = IKBIP Isoform 6 of MMS19 nucleotide excision repair protein108 kDa  4 0 0 1 homolog OS = Homo sapiens GN = MMS19 Isoform Short ofRNA-binding protein FUS OS = Homo 53 kDa 4 9 0 4 sapiens OX = 9606 GN =FUS Microtubule-actin cross-linking factor 1, isoforms 1/2/3/5 838 kDa 4 2 0 0 OS = Homo sapiens OX = 9606 GN = MACF1 PE = 1 SV = 4Mitochondrial carrier homolog 1 (Fragment) OS = Homo 43 kDa 4 0 0 5sapiens OX = 9606 GN = MTCH1 PE = 1 SV = 1 Nuclease-sensitiveelement-binding protein 1 OS = Homo 36 kDa 4 0 7 9 sapiens OX = 9606 GN= YBX1 PE = 1 SV = 3 Nucleolar GTP-binding protein 1 OS = Homo sapiens74 kDa 4 5 0 1 OX = 9606 GN = GTPBP4 PE = 1 SV = 3 OCIAdomain-containing protein 2 OS = Homo sapiens 17 kDa 4 0 0 0 OX = 9606GN = OCIAD2 PE = 1 SV = 1 Peroxisomal multifunctional enzyme type 2 OS =Homo 80 kDa 4 10 0 9 sapiens GN = HSD17B4 PE = 1 SV = 3 ProbableATP-dependent RNA helicase DDX6 54 kDa 4 4 0 2 OS = Homo sapiens OX =9606 GN = DDX6 PE = 1 SV = 2 Proteasome subunit alpha type-2 OS = Homosapiens 26 kDa 4 0 0 8 OX = 9606 GN = PSMA2 PE = 1 SV = 2 Proteasomesubunit beta type-1 OS = Homo sapiens 26 kDa 4 0 0 6 OX = 9606 GN =PSMB1 PE = 1 SV = 2 Proteasome subunit beta type-3 OS = Homo sapiens 23kDa 4 0 0 6 OX = 9606 GN = PSMB3 PE = 1 SV = 2 Proteasome subunit betatype-7 OS = Homo sapiens 30 kDa 4 0 0 6 OX = 9606 GN = PSMB7 PE = 1 SV =1 Protein arginine N-methyltransferase 1 OS = Homo 42 kDa 4 3 0 1sapiens GN = PRMT1 PE = 1 SV = 2 Ras GTPase-activating protein-bindingprotein 2 54 kDa 4 14 2 5 OS = Homo sapiens OX = 9606 GN = G3BP2 PE = 1SV = 2 Ras suppressor protein 1 OS = Homo sapiens OX = 9606 32 kDa 4 161 8 GN = RSU1 PE = 1 SV = 3 RNA-binding protein Raly OS = Homo sapiensOX = 9606 32 kDa 4 14 2 7 GN = RALY PE = 1 SV = 1 Sideroflexin-1 OS =Homo sapiens OX = 9606 GN = SFXN1 36 kDa 4 3 1 4 PE = 1 SV = 4Sideroflexin-3 OS = Homo sapiens OX = 9606 GN = SFXN3 36 kDa 4 0 0 4 PE= 1 SV = 3 Signal recognition particle 9 kDa protein OS = Homo 10 kDa 47 1 3 sapiens OX = 9606 GN = SRP9 PE = 1 SV = 2 Splicing factor 3Asubunit 1 OS = Homo sapiens 89 kDa 4 6 0 5 OX = 9606 GN = SF3A1 PE = 1SV = 1 SWISS-PROT:Q9TTE1 (Bos taurus) Endopin-1 46 kDa 4 4 2 1 precursorThy-1 membrane glycoprotein OS = Homo sapiens 18 kDa 4 0 6 3 OX = 9606GN = THY1 PE = 1 SV = 2 Transmembrane protein 43 OS = Homo sapiens OX =9606 45 kDa 4 0 5 6 GN = TMEM43 PE = 1 SV = 1 TREMBL:Q1A7A4 (Bos taurus)similar to complement 189 kDa  4 0 0 0 component C5 Tricarboxylatetransport protein, mitochondrial 34 kDa 4 2 0 3 OS = Homo sapiens OX =9606 GN = SLC25A1 PE = 1 SV = 2 U2 small nuclear ribonucleoprotein A′ OS= Homo sapiens 28 kDa 4 0 0 4 OX = 9606 GN = SNRPA1 PE = 1 SV = 2Vasodilator-stimulated phosphoprotein OS = Homo 40 kDa 4 10 0 10 sapiensOX = 9606 GN = VASP PE = 1 SV = 3 (Bos taurus) 47 kDa protein 47 kDa 3 00 0 (Bos taurus) similar to Complement C4-A precursor 193 kDa  3 7 4 426S proteasome non-ATPase regulatory subunit 5 56 kDa 3 4 0 1 OS = Homosapiens OX = 9606 GN = PSMD5 PE = 1 SV = 3 26S proteasome non-ATPaseregulatory subunit 7 37 kDa 3 10 0 6 OS = Homo sapiens OX = 9606 GN =PSMD7 PE = 1 SV = 2 26S proteasome regulatory subunit 7 OS = Homosapiens 49 kDa 3 4 0 4 OX = 9606 GN = PSMC2 PE = 1 SV = 3 39S ribosomalprotein L28, mitochondrial OS = Homo 30 kDa 3 0 0 1 sapiens OX = 9606 GN= MRPL28 PE = 1 SV = 4 40S ribosomal protein S15a OS = Homo sapiens OX =9606 15 kDa 3 0 2 3 GN = RPS15A PE = 1 SV = 2 60S ribosomal protein L15OS = Homo sapiens OX = 9606 24 kDa 3 27 3 11 GN = RPL15 PE = 1 SV = 2Acetyl-CoA acetyltransferase, mitochondrial OS = Homo 45 kDa 3 11 0 5sapiens OX = 9606 GN = ACAT1 PE = 1 SV = 1 ATP synthase F(0) complexsubunit B1, mitochondrial 29 kDa 3 0 0 2 OS = Homo sapiens OX = 9606 GN= ATP5PB PE = 1 SV = 2 ATP-binding cassette sub-family D member 3 OS =Homo 75 kDa 3 8 2 2 sapiens OX = 9606 GN = ABCD3 PE = 1 SV = 1Calponin-3 OS = Homo sapiens OX = 9606 GN = CNN3 36 kDa 3 4 1 6 PE = 1SV = 1 Cell surface glycoprotein MUC18 OS = Homo sapiens 72 kDa 3 3 0 6OX = 9606 GN = MCAM PE = 1 SV = 2 CTP synthase 1 OS = Homo sapiens OX =9606 67 kDa 3 0 0 0 GN = CTPS1 PE = 1 SV = 2 Cytoplasmic dynein 1 lightintermediate chain 1 57 kDa 3 11 0 4 OS = Homo sapiens OX = 9606 GN =DYNC1LI1 PE = 1 SV = 3 Cytoplasmic FMR1-interacting protein 1 OS = Homo145 kDa  3 2 0 0 sapiens OX = 9606 GN = CYFIP1 PE = 1 SV = 1Desmoglein-2 OS = Homo sapiens OX = 9606 GN = DSG2 122 kDa  3 17 5 7 PE= 1 SV = 2 Desmoplakin OS = Homo sapiens OX = 9606 GN = DSP 332 kDa  335 0 0 PE = 1 SV = 3 Dihydrolipoyllysine-residue acetyltransferasecomponent 69 kDa 3 0 2 10 of pyruvate dehydrogenase complex,mitochondrial OS = Homo sapiens OX = 9606 GN = DLAT PE = 1 SV = 3 DnaJhomolog subfamily A member 2 OS = Homo sapiens 46 kDa 3 0 1 6 OX = 9606GN = DNAJA2 PE = 1 SV = 1 Dynein light chain Tctex-type 1 OS = Homosapiens 12 kDa 3 13 3 5 OX = 9606 GN = DYNLT1 PE = 1 SV = 1 E3ubiquitin/ISG15 ligase TRIM25 OS = Homo sapiens 71 kDa 3 0 0 1 OX = 9606GN = TRIM25 PE = 1 SV = 2 EGF-like repeat and discoidin I-likedomain-containing 54 kDa 3 26 1 7 protein 3 OS = Homo sapiens OX = 9606GN = EDIL3 PE = 1 SV = 1 Enoyl-CoA hydratase, mitochondrial OS = Homosapiens 31 kDa 3 0 0 2 OX = 9606 GN = ECHS1 PE = 1 SV = 4 Epidermalgrowth factor receptor kinase substrate 8-like 81 kDa 3 4 0 5 protein 2OS = Homo sapiens OX = 9606 GN = EPS8L2 PE = 1 SV = 2 ER lumenprotein-retaining receptor 1 OS = Homo sapiens 25 kDa 3 0 2 1 OX = 9606GN = KDELR1 PE = 1 SV = 1 Eukaryotic translation initiation factor 2subunit 2 38 kDa 3 0 7 10 OS = Homo sapiens OX = 9606 GN = EIF2S2 PE = 1SV = 2 General transcription factor II-I OS = Homo sapiens 112 kDa  3 273 3 OX = 9606 GN = GTF2I PE = 1 SV = 2 High mobility group proteinHMGI-C OS = Homo sapiens 12 kDa 3 48 3 0 GN = HMGA2 PE = 1 SV = 1Inosine-5′-monophosphate dehydrogenase 2 OS = Homo 56 kDa 3 0 0 2sapiens OX = 9606 GN = IMPDH2 PE = 1 SV = 2 Isoform 1 of Apoptosisinhibitor 5 OS = Homo sapiens 49 kDa 3 5 0 3 OX = 9606 GN = API5 Isoform12 of Titin OS = Homo sapiens OX = 9606 3994 kDa  3 1 3 3 GN = TTNIsoform 2 of 3-hydroxyacyl-CoA dehydrogenase type-2 26 kDa 3 2 0 1 OS =Homo sapiens OX = 9606 GN = HSD17B10 Isoform 2 of ATP-dependent RNAhelicase DDX54 99 kDa 3 1 0 3 OS = Homo sapiens OX = 9606 GN = DDX54Isoform 2 of B-cell receptor-associated protein 31 35 kDa 3 1 4 7 OS =Homo sapiens OX = 9606 GN = BCAP31 Isoform 2 of Calcium-bindingmitochondrial carrier 51 kDa 3 1 0 5 protein SCaMC-1 OS = Homo sapiensOX = 9606 GN = SLC25A24 Isoform 2 of Collagen alpha-1(V) chain OS = Homo184 kDa  3 0 13 6 sapiens OX = 9606 GN = COL5A1 Isoform 2 of E3ubiquitin-protein ligase UBR4 576 kDa  3 0 0 1 OS = Homo sapiens OX =9606 GN = UBR4 Isoform 2 of Importin-4 OS = Homo sapiens GN = IPO4 119kDa  3 0 0 0 Isoform 2 of Insulin-like growth factor 2 mRNA-binding 62kDa 3 19 2 5 protein 2 OS = Homo sapiens GN = IGF2BP2 Isoform 2 of NADHdehydrogenase [ubiquinone] 1 alpha 49 kDa 3 0 0 2 subcomplex subunit 10,mitochondrial OS = Homo sapiens OX = 9606 GN = NDUFA10 Isoform 2 ofNADH-cytochrome b5 reductase 3 32 kDa 3 6 0 3 OS = Homo sapiens OX =9606 GN = CYB5R3 Isoform 2 of Nuclear pore complex protein Nup107 103kDa  3 1 0 0 OS = Homo sapiens OX = 9606 GN = NUP107 Isoform 2 ofProtein Dok-7 OS = Homo sapiens OX = 9606 37 kDa 3 0 2 5 GN = DOK7Isoform 2 of Protein FAM98B OS = Homo sapiens 46 kDa 3 9 0 4 OX = 9606GN = FAM98B Isoform 2 of Sacsin OS = Homo sapiens OX = 9606 437 kDa  3 00 0 GN = SACS Isoform 2 of Serine/arginine-rich splicing factor 3 14 kDa3 8 0 4 OS = Homo sapiens OX = 9606 GN = SRSF3 Isoform 2 of Smallnuclear ribonucleoprotein Sm D3 13 kDa 3 10 2 3 OS = Homo sapiens OX =9606 GN = SNRPD3 Isoform 2 of Stathmin OS = Homo sapiens OX = 9606 20kDa 3 0 0 3 GN = STMN1 Isoform 2 of TP53-binding protein 1 OS = Homosapiens 214 kDa  3 4 0 0 OX = 9606 GN = TP53BP1 Isoform 2 of Verylong-chain specific acyl-CoA 68 kDa 3 10 0 5 dehydrogenase,mitochondrial OS = Homo sapiens OX = 9606 GN = ACADVL Isoform 2 ofVoltage-dependent anion-selective channel 31 kDa 3 15 0 5 protein 3 OS =Homo sapiens OX = 9606 GN = VDAC3 Isoform 2 of V-type proton ATPasecatalytic subunit A 65 kDa 3 15 1 2 OS = Homo sapiens OX = 9606 GN =ATP6V1A Isoform 3 of 4F2 cell-surface antigen heavy chain 62 kDa 3 1 1 1OS = Homo sapiens GN = SLC3A2 Isoform 3 of Aldehyde dehydrogenase family16 member 80 kDa 3 0 0 1 A1 OS = Homo sapiens OX = 9606 GN = ALDH16A1Isoform 3 of Drebrin OS = Homo sapiens GN = DBN1 76 kDa 3 31 4 3 Isoform3 of Erbin OS = Homo sapiens OX = 9606 153 kDa  3 0 0 0 GN = ERBINIsoform 3 of Nucleoside diphosphate kinase B OS = Homo 30 kDa 3 12 0 7sapiens GN = NME2 Isoform 3 of Perilipin-3 OS = Homo sapiens OX = 960647 kDa 3 1 0 3 GN = PLIN3 Isoform 3 of SUN domain-containing protein 280 kDa 3 15 4 12 OS = Homo sapiens OX = 9606 GN = SUN2 Isoform 3 ofTransportin-3 OS = Homo sapiens OX = 9606 103 kDa  3 0 0 0 GN = TNPO3Isoform 4 of 26S proteasome non-ATPase regulatory 52 kDa 3 17 0 2subunit 6 OS = Homo sapiens OX = 9606 GN = PSMD6 Isoform 9 of Proteintransport protein Sec31A OS = Homo 131 kDa  3 3 0 1 sapiens GN = SEC31AIsoform K of Kinesin light chain 1 OS = Homo sapiens 70 kDa 3 2 0 2 OX =9606 GN = KLC1 MICOS complex subunit MIC19 OS = Homo sapiens 26 kDa 3 03 6 OX = 9606 GN = CHCHD3 PE = 1 SV = 1 Nuclear pore complex proteinNup205 OS = Homo 228 kDa  3 0 0 0 sapiens OX = 9606 GN = NUP205 PE = 1SV = 3 Nuclear pore complex protein Nup93 OS = Homo sapiens 93 kDa 3 6 03 OX = 9606 GN = NUP93 PE = 1 SV = 2 PC4 and SFRS1-interacting proteinOS = Homo sapiens 60 kDa 3 16 1 5 OX = 9606 GN = PSIP1 PE = 1 SV = 1Peroxiredoxin-6 OS = Homo sapiens OX = 9606 25 kDa 3 0 0 0 GN = PRDX6 PE= 1 SV = 3 PRA1 family protein 3 OS = Homo sapiens OX = 9606 22 kDa 3 71 4 GN = ARL6IP5 PE = 1 SV = 1 Proliferating cell nuclear antigen OS =Homo sapiens 29 kDa 3 0 0 0 OX = 9606 GN = PCNA PE = 1 SV = 1Proliferation-associated protein 2G4 OS = Homo sapiens 44 kDa 3 16 0 6OX = 9606 GN = PA2G4 PE = 1 SV = 3 Proteasome adapter and scaffoldprotein ECM29 204 kDa  3 0 0 2 OS = Homo sapiens OX = 9606 GN = ECPAS PE= 1 SV = 2 Proteasome subunit beta type-4 OS = Homo sapiens 29 kDa 3 0 04 OX = 9606 GN = PSMB4 PE = 1 SV = 4 Proteasome subunit beta type-6 OS =Homo sapiens 25 kDa 3 0 0 3 OX = 9606 GN = PSMB6 PE = 1 SV = 4 ProteinDEK OS = Homo sapiens OX = 9606 GN = DEK 43 kDa 3 2 0 2 PE = 1 SV = 1Protein S100-A11 OS = Homo sapiens OX = 9606 12 kDa 3 0 0 2 GN = S100A11PE = 1 SV = 2 Protein transport protein Sec61 subunit beta OS = Homo 10kDa 3 1 2 2 sapiens OX = 9606 GN = SEC61B PE = 1 SV = 2Puromycin-sensitive aminopeptidase OS = Homo sapiens 103 kDa  3 0 0 2 GN= NPEPPS PE = 1 SV = 2 Ras-related protein Rab-11B OS = Homo sapiens 24kDa 3 9 1 6 OX = 9606 GN = RAB11B PE = 1 SV = 4 Ras-related proteinRab-14 OS = Homo sapiens OX = 9606 24 kDa 3 0 0 6 GN = RAB14 PE = 1 SV =4 Ras-related protein Rap-1b OS = Homo sapiens OX = 9606 21 kDa 3 7 2 3GN = RAP1B PE = 1 SV = 1 Ras-related protein R-Ras OS = Homo sapiens OX= 9606 23 kDa 3 0 0 2 GN = RRAS PE = 1 SV = 1 RNA transcription,translation and transport factor 28 kDa 3 0 4 6 protein OS = Homosapiens OX = 9606 GN = RTRAF PE = 1 SV = 1 SAP domain-containingribonucleoprotein OS = Homo 24 kDa 3 0 0 2 sapiens OX = 9606 GN = SARNPPE = 1 SV = 3 SWISS-PROT:P06868 (Bos taurus) Plasminogen 91 kDa 3 1 5 0precursor SWISS-PROT:P41361 (Bos taurus) Antithrombin-III 52 kDa 3 3 0 0precursor TAR DNA-binding protein 43 OS = Homo sapiens 45 kDa 3 2 0 3 OX= 9606 GN = TARDBP PE = 1 SV = 1 Thioredoxin reductase 1, cytoplasmic OS= Homo sapiens 71 kDa 3 0 0 1 GN = TXNRD1 PE = 1 SV = 3 Thrombospondintype-1 domain-containing protein 4 112 kDa  3 22 10 1 OS = Homo sapiensOX = 9606 GN = THSD4 PE = 2 SV = 2 TREMBL:Q2KJF1 (Bos taurus) Alpha-1-Bglycoprotein 54 kDa 3 11 1 3 tRNA-splicing ligase RtcB homolog OS = Homosapiens 55 kDa 3 19 3 9 OX = 9606 GN = RTCB PE = 1 SV = 1 Tyrosine--tRNAligase, cytoplasmic OS = Homo sapiens 59 kDa 3 0 0 2 OX = 9606 GN = YARSPE = 1 SV = 4 Ubiquitin thioesterase OTUB1 OS = Homo sapiens 31 kDa 3 00 1 OX = 9606 GN = OTUB1 PE = 1 SV = 2 Vigilin OS = Homo sapiens OX =9606 GN = HDLBP PE = 1 141 kDa  3 9 0 2 SV = 2 V-type proton ATPase 116kDa subunit a isoform 3 93 kDa 3 16 1 2 OS = Homo sapiens OX = 9606 GN =TCIRG1 PE = 1 SV = 3 WD repeat-containing protein 61 OS = Homo sapiens34 kDa 3 0 1 4 OX = 9606 GN = WDR61 PE = 1 SV = 1 39S ribosomal proteinL41, mitochondrial OS = Homo 15 kDa 2 0 0 5 sapiens OX = 9606 GN =MRPL41 PE = 1 SV = 1 40S ribosomal protein S25 OS = Homo sapiens OX =9606 14 kDa 2 0 8 7 GN = RPS25 PE = 1 SV = 1 60S acidic ribosomalprotein P1 OS = Homo sapiens 12 kDa 2 13 3 5 OX = 9606 GN = RPLP1 PE = 1SV = 1 Actin-like protein 6A OS = Homo sapiens OX = 9606 47 kDa 2 12 0 2GN = ACTL6A PE = 1 SV = 1 Actin-related protein 2/3 complex subunit 5 OS= Homo 16 kDa 2 15 5 7 sapiens OX = 9606 GN = ARPC5 PE = 1 SV = 3Actin-related protein 2/3 complex subunit 5-like protein 17 kDa 2 0 6 5OS = Homo sapiens OX = 9606 GN = ARPC5L PE = 1 SV = 1 Activated RNApolymerase II transcriptional coactivator 14 kDa 2 1 0 2 p15 OS = Homosapiens OX = 9606 GN = SUB1 PE = 1 SV = 3 Alcohol dehydrogenase[NADP(+)] OS = Homo sapiens 37 kDa 2 0 0 1 OX = 9606 GN = AKR1A1 PE = 1SV = 3 Aspartyl/asparaginyl beta-hydroxylase OS = Homo 86 kDa 2 4 1 3sapiens OX = 9606 GN = ASPH PE = 1 SV = 3 ATP synthase subunit f,mitochondrial OS = Homo 11 kDa 2 0 2 2 sapiens OX = 9606 GN = ATP5MF PE= 1 SV = 1 ATP-binding cassette sub-family B member 6, 78 kDa 2 0 0 0mitochondrial (Fragment) OS = Homo sapiens OX = 9606 GN = ABCB6 PE = 1SV = 1 Catenin delta-1 OS = Homo sapiens OX = 9606 108 kDa  2 13 0 1 GN= CTNND1 PE = 1 SV = 1 Cathepsin B OS = Homo sapiens OX = 9606 GN = CTSB38 kDa 2 0 0 0 PE = 1 SV = 3 Caveolin-1 OS = Homo sapiens OX = 9606 GN =CAV1 20 kDa 2 11 2 4 PE = 1 SV = 4 Cell cycle and apoptosis regulatorprotein 2 OS = Homo 103 kDa  2 1 0 4 sapiens GN = CCAR2 PE = 1 SV = 2Centromere protein V OS = Homo sapiens OX = 9606 30 kDa 2 3 0 1 GN =CENPV PE = 1 SV = 1 Citrate synthase OS = Homo sapiens OX = 9606 GN = CS50 kDa 2 0 0 3 PE = 1 SV = 1 Cytochrome b-cl complex subunit 1,mitochondrial 53 kDa 2 0 0 1 OS = Homo sapiens OX = 9606 GN = UQCRC1 PE= 1 SV = 3 Cytochrome c Oxidase subunit 7A2, mitochondrial 9 kDa 2 0 0 1OS = Homo sapiens OX = 9606 GN = COX7A2 PE = 1 SV = 1 Dystonin OS = Homosapiens OX = 9606 GN = DST PE = 1 861 kDa  2 0 0 0 SV = 4 EHdomain-containing protein 2 OS = Homo sapiens 61 kDa 2 2 0 3 OX = 9606GN = EHD2 PE = 1 SV = 2 Enhancer of mRNA-decapping protein 4 OS = Homo152 kDa  2 0 0 0 sapiens GN = EDC4 PE = 1 SV = 1 Eukaryotic translationinitiation factor 5B OS = Homo 139 kDa  2 0 0 2 sapiens OX = 9606 GN =EIF5B PE = 1 SV = 4 Exosome RNA helicase MTR4 OS = Homo sapiens 118 kDa 2 0 0 1 OX = 9606 GN = MTREX PE = 1 SV = 3 Far upstream element-bindingprotein 2 OS = Homo 73 kDa 2 0 0 0 sapiens OX = 9606 GN = KHSRP PE = 1SV = 4 Ferritin heavy chain OS = Homo sapiens OX = 9606 21 kDa 2 0 0 1GN = FTH1 PE = 1 SV = 2 FH2 domain-containing protein 1 OS = Homosapiens 125 kDa  2 0 0 0 OX = 9606 GN = FHDC1 PE = 1 SV = 2Glutaredoxin-3 OS = Homo sapiens OX = 9606 37 kDa 2 0 0 0 GN = GLRX3 PE= 1 SV = 2 GTP-binding protein SAR1a OS = Homo sapiens 22 kDa 2 0 0 3 OX= 9606 GN = SAR1A PE = 1 SV = 1 Guanine nucleotide-binding proteinG(I)/G(S)/G(T) 37 kDa 2 14 0 0 subunit beta-2 OS = Homo sapiens OX =9606 GN = GNB2 PE = 1 SV = 3 Heterogeneous nuclear ribonucleoproteinU-like protein 2 85 kDa 2 8 0 2 OS = Homo sapiens OX = 9606 GN =HNRNPUL2 PE = 1 SV = 1 High mobility group protein B3 OS = Homo sapiens23 kDa 2 0 0 3 OX = 9606 GN = HMGB3 PE = 1 SV = 4 Inversin OS = Homosapiens OX = 9606 GN = INVS PE = 1 118 kDa  2 0 0 0 SV = 2 Isoform 10 ofCD44 antigen OS = Homo sapiens 53 kDa 2 27 5 2 OX = 9606 GN = CD44Isoform 2 of 40S ribosomal protein S20 OS = Homo 16 kDa 2 7 1 3 sapiensOX = 9606 GN = RPS20 Isoform 2 of AP-2 complex subunit alpha-2 OS = Homo104 kDa  2 8 0 3 sapiens OX = 9606 GN = AP2A2 Isoform 2 of ATP-bindingcassette sub-family F member 92 kDa 2 6 2 2 1 OS = Homo sapiens OX =9606 GN = ABCF1 Isoform 2 of cAMP-dependent protein kinase type II- 43kDa 2 11 0 3 alpha regulatory subunit OS = Homo sapiens OX = 9606 GN =PRKAR2A Isoform 2 of Chromatin target of PRMT1 protein 27 kDa 2 4 2 2 OS= Homo sapiens OX = 9606 GN = CHTOP Isoform 2 of Collagen alpha-1(XXII)chain OS = Homo 159 kDa  2 0 0 1 sapiens OX = 9606 GN = COL22A1 Isoform2 of DNA repair protein RAD50 OS = Homo 155 kDa  2 0 0 1 sapiens OX =9606 GN = RAD50 Isoform 2 of E3 ubiquitin-protein ligase RNF213 596 kDa 2 0 0 1 OS = Homo sapiens OX = 9606 GN = RNF213 Isoform 2 of Electrontransfer flavoprotein subunit alpha, 30 kDa 2 1 0 5 mitochondrial OS =Homo sapiens OX = 9606 GN = ETFA Isoform 2 of Enoyl-CoA delta isomerase2, mitochondrial 40 kDa 2 0 0 0 OS = Homo sapiens OX = 9606 GN = ECI2Isoform 2 of Eukaryotic peptide chain release factor 45 kDa 2 2 0 2subunit 1 OS = Homo sapiens OX = 9606 GN = ETF1 Isoform 2 of Eukaryotictranslation initiation factor 3 58 kDa 2 2 0 0 subunit D OS = Homosapiens OX = 9606 GN = EIF3D Isoform 2 of Fermitin family homolog 2 OS =Homo 72 kDa 2 0 0 6 sapiens OX = 9606 GN = FERMT2 Isoform 2 ofGlutamine--fructose-6-phosphate 77 kDa 2 5 0 1 aminotransferase[isomerizing] 1 OS = Homo sapiens OX = 9606 GN = GFPT1 Isoform 2 ofGuanine nucleotide-binding protein-like 3 61 kDa 2 3 0 0 OS = Homosapiens OX = 9606 GN = GNL3 Isoform 2 of Histidine--tRNA ligase,cytoplasmic 53 kDa 2 3 0 4 OS = Homo sapiens OX = 9606 GN = HARS Isoform2 of Histone H1.0 OS = Homo sapiens OX = 9606 19 kDa 2 1 3 3 GN = H1F0Isoform 2 of Interferon-induced, double-stranded RNA- 57 kDa 2 1 0 1activated protein kinase OS = Homo sapiens OX = 9606 GN = EIF2AK2Isoform 2 of Neurotrimin OS = Homo sapiens OX = 9606 38 kDa 2 0 0 0 GN =NTM Isoform 2 of Neutral cholesterol ester hydrolase 1 47 kDa 2 1 0 3 OS= Homo sapiens OX = 9606 GN = NCEH1 Isoform 2 of Nucleosome assemblyprotein 1-like 1 43 kDa 2 10 1 3 OS = Homo sapiens OX = 9606 GN = NAP1L1Isoform 2 of Nucleosome assembly protein 1-like 4 44 kDa 2 0 0 3 OS =Homo sapiens OX = 9606 GN = NAP1L4 Isoform 2 of Polyadenylate-bindingprotein 2 OS = Homo 31 kDa 2 7 0 2 sapiens OX = 9606 GN = PABPN1 Isoform2 of Pre-mRNA-splicing factor SYF2 OS = Homo 24 kDa 2 0 0 3 sapiens OX =9606 GN = SYF2 Isoform 2 of Procollagen-lysine, 2-oxoglutarate 5- 88 kDa2 12 0 1 dioxygenase 1 OS = Homo sapiens OX = 9606 GN = PLOD1 Isoform 2of Procollagen-lysine, 2-oxoglutarate 5- 87 kDa 2 2 0 0 dioxygenase 2 OS= Homo sapiens OX = 9606 GN = PLOD2 Isoform 2 of Protein enabled homologOS = Homo sapiens 64 kDa 2 26 1 18 OX = 9606 GN = ENAH Isoform 2 ofProtein SGT1 homolog OS = Homo sapiens 38 kDa 2 0 0 0 OX = 9606 GN =SUGT1 Isoform 2 of Protein transport protein Sec16A OS = Homo 229 kDa  20 0 1 sapiens OX = 9606 GN = SEC16A Isoform 2 of RNA-binding proteinwith serine-rich 32 kDa 2 3 1 2 domain 1 OS = Homo sapiens OX = 9606 GN= RNPS1 Isoform 2 of Signal recognition particle subunit SRP68 67 kDa 29 0 0 OS = Homo sapiens OX = 9606 GN = SRP68 Isoform 2 of Syntenin-1 OS= Homo sapiens OX = 9606 32 kDa 2 6 0 0 GN = SDCBP Isoform 2 ofTranslocating chain-associated membrane 40 kDa 2 6 4 5 protein 1 OS =Homo sapiens OX = 9606 GN = TRAM1 Isoform 2 of Trifunctional enzymesubunit beta, 49 kDa 2 51 9 10 mitochondrial OS = Homo sapiens OX = 9606GN = HADHB Isoform 2 of UDP-glucose:glycoprotein 175 kDa  2 2 0 1glucosyltransferase 1 OS = Homo sapiens OX = 9606 GN = UGGT1 Isoform 3of 28S ribosomal protein S29, mitochondrial 42 kDa 2 5 2 1 OS = Homosapiens GN = DAP3 Isoform 3 of Basic leucine zipper and W2 domain- 51kDa 2 1 0 4 containing protein 1 OS = Homo sapiens OX = 9606 GN = BZW1Isoform 3 of E3 ubiquitin-protein ligase HUWE1 481 kDa  2 4 0 0 OS =Homo sapiens GN = HUWE1 Isoform 3 of Heterogeneous nuclearribonucleoprotein 32 kDa 2 8 0 3 H3 OS = Homo sapiens GN = HNRNPH3Isoform 3 of Integrin-linked protein kinase OS = Homo 36 kDa 2 0 0 2sapiens OX = 9606 GN = ILK Isoform 3 of Protein virilizer homolog OS =Homo sapiens 201 kDa  2 0 0 0 OX = 9606 GN = VIRMA Isoform 3 ofUbiquitin-associated protein 2-like 113 kDa  2 7 0 7 OS = Homo sapiensOX = 9606 GN = UBAP2L Isoform 4 of LIM domain and actin-binding protein1 85 kDa 2 49 6 4 OS = Homo sapiens OX = 9606 GN = LIMA1 Isoform 4 ofWASH complex subunit 2C OS = Homo 145 kDa  2 0 0 0 sapiens OX = 9606 GN= WASHC2C Isoform Delta 10 of Calcium/calmodulin-dependent 56 kDa 2 5 00 protein kinase type II subunit delta OS = Homo sapiens OX = 9606 GN =CAMK2D Isoform LAMP-2B of Lysosome-associated membrane 45 kDa 2 0 0 2glycoprotein 2 OS = Homo sapiens OX = 9606 GN = LAMP2 Leucine-richrepeat flightless-interacting protein 1 89 kDa 2 0 0 1 OS = Homo sapiensOX = 9606 GN = LRRFIP1 PE = 1 SV = 2 Lysophospholipid acyltransferase 7OS = Homo sapiens 53 kDa 2 2 0 0 GN = MBOAT7 PE = 1 SV = 2 Microsomalglutathione 5-transferase 3 OS = Homo 17 kDa 2 1 1 2 sapiens OX = 9606GN = MGST3 PE = 1 SV = 1 Mitochondrial import receptor subunit TOM40homolog 38 kDa 2 0 0 0 OS = Homo sapiens OX = 9606 GN = TOMM40 PE = 1 SV= 1 Mucin-19 OS = Homo sapiens OX = 9606 GN = MUC19 805 kDa  2 0 0 0 PE= 1 SV = 3 NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, 30 kDa2 4 0 4 mitochondrial OS = Homo sapiens OX = 9606 GN = NDUFS3 PE = 1 SV= 1 Nascent polypeptide-associated complex subunit alpha, 205 kDa  2 6 04 muscle-specific form OS = Homo sapiens OX = 9606 GN = NACA PE = 1 SV =1 Nicotinamide phosphoribosyltransferase OS = Homo 56 kDa 2 2 0 3sapiens OX = 9606 GN = NAMPT PE = 1 SV = 1 Non-histone chromosomalprotein HMG-14 OS = Homo 12 kDa 2 11 3 6 sapiens OX = 9606 GN = HMGN1 PE= 1 SV = 1 Nucleolar protein 11 OS = Homo sapiens OX = 9606 81 kDa 2 0 00 GN = NOL11 PE = 1 SV = 1 Obg-like ATPase 1 OS = Homo sapiens OX = 960645 kDa 2 4 0 3 GN = OLA1 PE = 1 SV = 2 PDZ and LIM domain protein 5 OS =Homo sapiens 64 kDa 2 3 0 2 GN = PDLIM5 PE = 1 SV = 5 Peroxiredoxin-2 OS= Homo sapiens OX = 9606 22 kDa 2 7 0 1 GN = PRDX2 PE = 1 SV = 5Phospholipid-transporting ATPase IB OS = Homo sapiens 129 kDa  2 0 2 0OX = 9606 GN = ATP8A2 PE = 1 SV = 3 Phosphoserine aminotransferase OS =Homo sapiens 40 kDa 2 0 0 0 OX = 9606 GN = PSAT1 PE = 1 SV = 2Plasminogen activator inhibitor 1 OS = Homo sapiens 45 kDa 2 90 41 29 OX= 9606 GN = SERPINE1 PE = 1 SV = 1 pre-rRNA processing protein FTSJ3 OS= Homo sapiens 97 kDa 2 3 0 1 OX = 9606 GN = FTSJ3 PE = 1 SV = 2 PRKCapoptosis WT1 regulator protein OS = Homo 37 kDa 2 0 1 6 sapiens OX =9606 GN = PAWR PE = 1 SV = 1 Proteasome activator complex subunit 2 OS =Homo 27 kDa 2 0 0 2 sapiens OX = 9606 GN = PSME2 PE = 1 SV = 4Proteasome subunit alpha type-5 OS = Homo sapiens 26 kDa 2 2 0 2 OX =9606 GN = PSMA5 PE = 1 SV = 3 Proteasome subunit alpha type-7 OS = Homosapiens 28 kDa 2 7 0 2 OX = 9606 GN = PSMA7 PE = 1 SV = 1 Proteasomesubunit beta type-2 OS = Homo sapiens 23 kDa 2 0 0 4 OX = 9606 GN =PSMB2 PE = 1 SV = 1 Protein flightless-1 homolog OS = Homo sapiens 145kDa  2 0 0 1 OX = 9606 GN = FLII PE = 1 SV = 2 Protein SEC13 homolog OS= Homo sapiens OX = 9606 36 kDa 2 4 0 4 GN = SEC13 PE = 1 SV = 3Ras-related protein Rab-5C OS = Homo sapiens OX = 9606 23 kDa 2 7 0 3 GN= RAB5C PE = 1 SV = 2 Ras-related protein Rab-7a OS = Homo sapiens OX =9606 23 kDa 2 0 0 2 GN = RAB7A PE = 1 SV = 1 Remodeling and spacingfactor 1 OS = Homo sapiens 164 kDa  2 9 0 0 GN = RSF1 PE = 1 SV = 2Serine beta-lactamase-like protein LACTB, 61 kDa 2 13 5 3 mitochondrialOS = Homo sapiens OX = 9606 GN = LACTB PE = 1 SV = 2Serine/arginine-rich splicing factor 1 OS = Homo sapiens 28 kDa 2 7 0 2GN = SRSF1 PE = 1 SV = 2 Structural maintenance of chromosomes protein1A 143 kDa  2 7 0 0 OS = Homo sapiens OX = 9606 GN = SMC1A PE = 1 SV = 2SWISS-PROT:P02777 (Bos taurus) similar to Platelet 24 kDa 2 3 3 3 factor4 SWISS-PROT:Q2UVX4 (Bos taurus) Complement C3 187 kDa  2 7 6 4precursor SWISS-PROT:Q95121 (Bos taurus) Pigment epithelium- 46 kDa 2 03 3 derived factor precursor Transaldolase OS = Homo sapiens OX = 960638 kDa 2 0 0 3 GN = TALDO1 PE = 1 SV = 2 Translationally-controlledtumor protein OS = Homo 20 kDa 2 0 0 2 sapiens OX = 9606 GN = TPT1 PE =1 SV = 1 Translocon-associated protein subunit alpha OS = Homo 32 kDa 25 3 5 sapiens OX = 9606 GN = SSR1 PE = 1 SV = 3 Transmembrane emp24domain-containing protein 10 25 kDa 2 0 6 8 OS = Homo sapiens OX = 9606GN = TMED10 PE = 1 SV = 2 Transmembrane emp24 domain-containing protein9 27 kDa 2 0 1 2 OS = Homo sapiens OX = 9606 GN = TMED9 PE = 1 SV = 2TREMBL:Q1RMN8 (Bos taurus) Similar to 25 kDa 2 0 0 0 Immunoglobulinlambda-like polypeptide 1 Tripeptidyl-peptidase 1 OS = Homo sapiens GN =TPP1 61 kDa 2 0 2 3 PE = 1 SV = 2 1,4-alpha-glucan-branching enzyme OS =Homo sapiens 80 kDa 1 0 0 3 OX = 9606 GN = GBE1 PE = 1 SV = 31-phosphatidylinositol 4,5-bisphosphate 139 kDa  1 0 0 2phosphodiesterase beta-3 OS = Homo sapiens GN = PLCB3 PE = 1 SV = 2 28Sribosomal protein S35, mitochondrial OS = Homo 37 kDa 1 2 0 2 sapiens GN= MRPS35 PE = 1 SV = 1 40S ribosomal protein S30 OS = Homo sapiens OX =9606  7 kDa 1 0 3 2 GN = FAU PE = 1 SV = 1 60S ribosomal protein L11 OS= Homo sapiens OX = 9606 20 kDa 1 18 1 3 GN = RPL11 PE = 1 SV = 2 60Sribosomal protein L35a OS = Homo sapiens 13 kDa 1 0 2 2 OX = 9606 GN =RPL35A PE = 1 SV = 2 60S ribosomal protein L37a OS = Homo sapiens 10 kDa1 0 5 5 OX = 9606 GN = RPL37A PE = 1 SV = 2 Alpha-parvin OS = Homosapiens OX = 9606 GN = PARVA 42 kDa 1 3 0 4 PE = 1 SV = 1 AT-richinteractive domain-containing protein 1A 242 kDa  1 3 0 0 OS = Homosapiens OX = 9606 GN = ARID1A PE = 1 SV = 3 Basigin OS = Homo sapiens OX= 9606 GN = BSG PE = 1 42 kDa 1 3 0 1 SV = 2 Biorientation ofchromosomes in cell division protein 1- 330 kDa  1 0 0 3 like 1 OS =Homo sapiens OX = 9606 GN = BOD1L1 PE = 1 SV = 2 Cell division cycle5-like protein OS = Homo sapiens 92 kDa 1 3 0 4 OX = 9606 GN = CDC5L PE= 1 SV = 2 Coactosin-like protein OS = Homo sapiens OX = 9606 16 kDa 1 00 2 GN = COTL1 PE = 1 SV = 3 Collagen alpha-1(III) chain OS = Homosapiens OX = 9606 139 kDa  1 2 0 1 GN = COL3A1 PE = 1 SV = 4 Collagenalpha-2(I) chain OS = Homo sapiens OX = 9606 129 kDa  1 12 7 3 GN =COL1A2 PE = 1 SV = 7 Collagen alpha-2(V) chain OS = Homo sapiens OX =9606 145 kDa  1 0 15 6 GN = COL5A2 PE = 1 SV = 3 Collagen alpha-6(VI)chain OS = Homo sapiens OX = 9606 247 kDa  1 0 3 2 GN = COL6A6 PE = 1 SV= 2 Complement component 1 Q subcomponent-binding 31 kDa 1 0 0 4protein, mitochondrial OS = Homo sapiens OX = 9606 GN = C1QBP PE = 1 SV= 1 Cysteine and glycine-rich protein 1 OS = Homo sapiens 21 kDa 1 0 0 2OX = 9606 GN = CSRP1 PE = 1 SV = 3 Cytochrome b-c1 complex subunit 9 OS= Homo sapiens  7 kDa 1 2 0 2 OX = 9606 GN = UQCR10 PE = 1 SV = 3Cytochrome c1, heme protein, mitochondrial OS = Homo 35 kDa 1 0 0 3sapiens OX = 9606 GN = CYC1 PE = 1 SV = 3 DDRGK domain-containingprotein 1 OS = Homo sapiens 36 kDa 1 0 1 2 OX = 9606 GN = DDRGK1 PE = 1SV = 2 Destrin OS = Homo sapiens OX = 9606 GN = DSTN PE = 1 19 kDa 1 3 06 SV = 3 Dihydrolipoyl dehydrogenase, mitochondrial OS = Homo 54 kDa 1 80 2 sapiens OX = 9606 GN = DLD PE = 1 SV = 2 DNA-directed RNApolymerases I, II, and III subunit 25 kDa 1 0 5 1 RPABC1 OS = Homosapiens OX = 9606 GN = POLR2E PE = 1 SV = 4 ELAV-like protein 1 OS =Homo sapiens OX = 9606 36 kDa 1 3 0 1 GN = ELAVL1 PE = 1 SV = 2Eukaryotic translation initiation factor 3 subunit G 36 kDa 1 0 0 3 OS =Homo sapiens OX = 9606 GN = EIF3G PE = 1 SV = 2 Farnesyl pyrophosphatesynthase OS = Homo sapiens 48 kDa 1 3 0 1 OX = 9606 GN = FDPS PE = 1 SV= 4 Fibrillin-1 OS = Homo sapiens OX = 9606 GN = FBN1 312 kDa  1 0 2 0PE = 1 SV = 3 Fumarate hydratase, mitochondrial OS = Homo sapiens 55 kDa1 0 0 2 GN = FH PE = 1 SV = 3 Growth/differentiation factor 15 OS = Homosapiens 34 kDa 1 0 7 6 OX = 9606 GN = GDF15 PE = 1 SV = 3 Histonedeacetylase complex subunit SAP18 OS = Homo 20 kDa 1 0 0 2 sapiens OX =9606 GN = SAP18 PE = 1 SV = 1 Histone H1x OS = Homo sapiens OX = 9606 GN= H1FX 22 kDa 1 0 0 2 PE = 1 SV = 1 Hydrocephalus-inducing proteinhomolog OS = Homo 576 kDa  1 0 1 0 sapiens OX = 9606 GN = HYDIN PE = 1SV = 3 Insulin-like growth factor 2 mRNA-binding protein 3 64 kDa 1 18 20 OS = Homo sapiens GN = IGF2BP3 PE = 1 SV = 2 Isoform 2 of Adipocyteplasma membrane-associated 32 kDa 1 0 0 3 protein OS = Homo sapiens OX =9606 GN = APMAP Isoform 2 of Aspartate aminotransferase, mitochondrial43 kDa 1 0 0 3 OS = Homo sapiens OX = 9606 GN = GOT2 Isoform 2 ofBH3-interacting domain death agonist 27 kDa 1 4 0 0 OS = Homo sapiens OX= 9606 GN = BID Isoform 2 of DnaJ homolog subfamily A member 3, 50 kDa 10 0 3 mitochondrial OS = Homo sapiens OX = 9606 GN = DNAJA3 Isoform 2 ofGlia-derived nexin OS = Homo sapiens 44 kDa 1 8 0 0 OX = 9606 GN =SERPINE2 Isoform 2 of Histone deacetylase 2 OS = Homo sapiens 52 kDa 1 40 1 OX = 9606 GN = HDAC2 Isoform 2 of Lysosomal protective protein OS =Homo 52 kDa 1 1 0 2 sapiens OX = 9606 GN = CTSA Isoform 2 of Lysosomemembrane protein 2 OS = Homo 38 kDa 1 4 0 0 sapiens OX = 9606 GN =SCARB2 Isoform 2 of Myosin phosphatase Rho-interacting protein 118 kDa 1 13 0 0 OS = Homo sapiens OX = 9606 GN = MPRIP Isoform 2 of NADHdehydrogenase [ubiquinone] 1 alpha 25 kDa 1 13 1 3 subcomplex subunit 13OS = Homo sapiens OX = 9606 GN = NDUFA13 Isoform 2 of NADH dehydrogenase[ubiquinone] iron- 52 kDa 1 5 0 1 sulfur protein 2, mitochondrial OS =Homo sapiens OX = 9606 GN = NDUFS2 Isoform 2 of Nesprin-2 OS = Homosapiens OX = 9606 799 kDa  1 12 1 0 GN = SYNE2 Isoform 2 of Nuclear porecomplex protein Nup214 213 kDa  1 0 0 3 OS = Homo sapiens OX = 9606 GN =NUP214 Isoform 2 of Phenylalanine--tRNA ligase alpha subunit 54 kDa 1 71 4 OS = Homo sapiens OX = 9606 GN = FARSA Isoform 2 of Probable 28SrRNA (cytosine(4447)-C(5))- 89 kDa 1 6 0 6 methyltransferase OS = Homosapiens OX = 9606 GN = NOP2 Isoform 2 of Protein FAM98A OS = Homosapiens 55 kDa 1 2 0 3 OX = 9606 GN = FAM98A Isoform 2 of Protein TFG OS= Homo sapiens OX = 9606 43 kDa 1 4 0 4 GN = TFG Isoform 2 of Pyruvatedehydrogenase E1 component 37 kDa 1 7 0 3 subunit beta, mitochondrial OS= Homo sapiens OX = 9606 GN = PDHB Isoform 2 of UTP--glucose-1-phosphate56 kDa 1 3 0 7 uridylyltransferase OS = Homo sapiens OX = 9606 GN = UGP2Isoform 2 of V-type proton ATPase 116 kDa subunit a 96 kDa 1 11 0 3isoform 1 OS = Homo sapiens OX = 9606 GN = ATP6V0A1 Isoform 2B ofCytoplasmic dynein 1 intermediate chain 2 71 kDa 1 16 1 3 OS = Homosapiens OX = 9606 GN = DYNC1I2 Isoform 3 of Activating signalcointegrator 1 complex 77 kDa 1 6 0 1 subunit 2 OS = Homo sapiens OX =9606 GN = ASCC2 Isoform 3 of Apoptosis-inducing factor 1, mitochondrial66 kDa 1 3 0 4 OS = Homo sapiens OX = 9606 GN = AIFM1 Isoform 3 ofCalumenin OS = Homo sapiens OX = 9606 38 kDa 1 1 0 2 GN = CALU Isoform 3of Fragile X mental retardation syndrome- 60 kDa 1 4 0 1 related protein1 OS = Homo sapiens OX = 9606 GN = FXR1 Isoform 3 of GRB10-interactingGYP protein 2 149 kDa  1 0 0 0 OS = Homo sapiens OX = 9606 GN = GIGYF2Isoform 3 of Pyrroline-5-carboxylate reductase 1, 36 kDa 1 8 0 0mitochondrial OS = Homo sapiens OX = 9606 GN = PYCR1 Isoform 3 ofRas-related protein R-Ras2 OS = Homo 20 kDa 1 5 0 1 sapiens GN = RRAS2Isoform 3 of Signal peptidase complex catalytic subunit 21 kDa 1 2 1 3SEC11A OS = Homo sapiens OX = 9606 GN = SEC11A Isoform 4 of Cadherin-13OS = Homo sapiens OX = 9606 83 kDa 1 23 6 2 GN = CDH13 Isoform 4 ofDipeptidyl peptidase 3 OS = Homo sapiens 79 kDa 1 0 0 2 OX = 9606 GN =DPP3 Isoform 4 of Dynamin-1-like protein OS = Homo sapiens 81 kDa 1 2 02 OX = 9606 GN = DNM1L Isoform 4 of Dynamin-2 OS = Homo sapiens OX =9606 98 kDa 1 13 0 6 GN = DNM2 Isoform 4 of Nexilin OS = Homo sapiens OX= 9606 73 kDa 1 28 12 7 GN = NEXN Isoform 5 of Obscurin OS = Homosapiens OX = 9606 925 kDa  1 0 0 1 GN = OBSCN Isoform 6 of RNA-bindingprotein EWS OS = Homo 63 kDa 1 6 0 6 sapiens OX = 9606 GN = EWSR1Isoform Heart of ATP synthase subunit gamma, 33 kDa 1 6 0 4mitochondrial OS = Homo sapiens OX = 9606 GN = ATP5F1C IsoformMitochondrial of Lysine--tRNA ligase 71 kDa 1 4 0 1 OS = Homo sapiens OX= 9606 GN = KARS Leucine-rich repeat-containing protein 17 OS = Homo 52kDa 1 11 9 4 sapiens OX = 9606 GN = LRRC17 PE = 2 SV = 1 Leucyl-cystinylaminopeptidase OS = Homo sapiens 117 kDa  1 4 3 2 OX = 9606 GN = LNPEPPE = 1 SV = 3 LIM and SH3 domain protein 1 OS = Homo sapiens 30 kDa 1 10 3 OX = 9606 GN = LASP1 PE = 1 SV = 2 Matrix metalloproteinase-14 OS =Homo sapiens 66 kDa 1 0 0 2 OX = 9606 GN = MMP14 PE = 1 SV = 3Microtubule-associated protein RP/EB family member 1 30 kDa 1 4 0 4 OS =Homo sapiens OX = 9606 GN = MAPRE1 PE = 1 SV = 3 Mitochondrial2-oxoglutarate/malate carrier protein 34 kDa 1 7 0 1 OS = Homo sapiensOX = 9606 GN = SLC25A11 PE = 1 SV = 3 Mitochondrial fission 1 protein OS= Homo sapiens 17 kDa 1 0 0 3 OX = 9606 GN = FIS1 PE = 1 SV = 2 NADHdehydrogenase [ubiquinone] 1 alpha subcomplex 17 kDa 1 6 1 2 subunit 12OS = Homo sapiens OX = 9606 GN = NDUFA12 PE = 1 SV = 1 PDZdomain-containing protein 4 OS = Homo sapiens 86 kDa 1 0 0 0 OX = 9606GN = PDZD4 PE = 1 SV = 1 Peroxisomal membrane protein PEX14 OS = Homo 41kDa 1 3 0 2 sapiens OX = 9606 GN = PEX14 PE = 1 SV = 1Platelet-activating factor acetylhydrolase IB subunit beta 26 kDa 1 6 01 OS = Homo sapiens GN = PAFAH1B2 PE = 1 SV = 1 Podocalyxin OS = Homosapiens GN = PODXL PE = 1 59 kDa 1 0 0 2 SV = 2 Prolyl 3-hydroxylase 3OS = Homo sapiens OX = 9606 82 kDa 1 0 0 2 GN = P3H3 PE = 1 SV = 1Prolyl 4-hydroxylase subunit alpha-2 OS = Homo sapiens 61 kDa 1 2 0 0 OX= 9606 GN = P4HA2 PE = 1 SV = 1 Proteasome subunit alpha type-6 OS =Homo sapiens 27 kDa 1 8 0 1 OX = 9606 GN = PSMA6 PE = 1 SV = 1Proteasome subunit beta type-5 OS = Homo sapiens 28 kDa 1 2 0 0 GN =PSMB5 PE = 1 SV = 3 Protein CYR61 OS = Homo sapiens OX = 9606 42 kDa 1 027 24 GN = CYR61 PE = 1 SV = 1 Protein mago nashi homolog OS = Homosapiens 17 kDa 1 7 0 0 OX = 9606 GN = MAGOH PE = 1 SV = 1 RNA-bindingprotein 14 OS = Homo sapiens OX = 9606 69 kDa 1 18 2 5 GN = RBM14 PE = 1SV = 2 RuvB-like 1 OS = Homo sapiens OX = 9606 GN = RUVBL1 50 kDa 1 18 02 PE = 1 SV = 1 Splicing factor 3B subunit 4 OS = Homo sapiens 44 kDa 10 0 2 OX = 9606 GN = SF3B4 PE = 1 SV = 1 Splicing factor U2AF 26 kDasubunit OS = Homo sapiens  4 kDa 1 0 2 1 OX = 9606 GN = U2AF1L4 PE = 4SV = 1 SWISS-PROT:P04258 (Bos taurus) Similar to Collagen 138 kDa  1 9 00 alpha 1(III) chain TREMBL:A2I7N3; Q27984 (Bos taurus) SERPINA3-7 47kDa 1 5 1 1 Unconventional myosin-Ie OS = Homo sapiens OX = 9606 127kD a 1 20 5 4 GN = MYO1E PE = 1 SV = 2 Vacuolar proteinsorting-associated protein VTA1 34 kDa 1 0 0 2 homolog OS = Homo sapiensOX = 9606 GN = VTA1 PE = 1 SV = 1 Very-long-chain (3R)-3-hydroxyacyl-CoAdehydratase 3 43 kDa 1 8 1 2 OS = Homo sapiens GN = HACD3 PE = 1 SV = 2von Willebrand factor A domain-containing protein 1 47 kDa 1 7 0 0 OS =Homo sapiens OX = 9606 GN = VWA1 PE = 1 SV = 1 (Bos taurus) 63 kDaprotein 63 kDa 0 55 0 0 10 kDa heat shock protein, mitochondrial OS =Homo 11 kDa 0 6 0 0 sapiens GN = HSPE1 PE = 1 SV = 21-phosphatidylinositol 3-phosphate 5-kinase OS = Homo 237 kDa  0 0 0 2sapiens OX = 9606 GN = PIKFYVE PE = 1 SV = 3 26S protease regulatorysubunit 10B OS = Homo sapiens 46 kDa 0 9 0 0 GN = PSMC6 PE = 1 SV = 126S protease regulatory subunit 6A OS = Homo sapiens 49 kDa 0 30 0 0 GN= PSMC3 PE = 1 SV = 3 26S proteasome non-ATPase regulatory subunit 14 35kDa 0 17 0 0 OS = Homo sapiens GN = PSMD14 PE = 1 SV = 1 26S proteasomenon-ATPase regulatory subunit 8 33 kDa 0 3 0 0 OS = Homo sapiens GN =PSMD8 PE = 1 SV = 1 28S ribosomal protein S34, mitochondrial OS = Homo26 kDa 0 2 0 0 sapiens GN = MRPS34 PE = 1 SV = 2 39S ribosomal proteinL11, mitochondrial OS = Homo 21 kDa 0 7 0 1 sapiens GN = MRPL11 PE = 1SV = 1 39S ribosomal protein L34, mitochondrial OS = Homo 20 kDa 0 8 0 0sapiens GN = MRPL34 PE = 1 SV = 1 3-ketoacyl-CoA thiolase, mitochondrialOS = Homo 42 kDa 0 0 0 2 sapiens OX = 9606 GN = ACAA2 PE = 1 SV = 2 40Sribosomal protein S13 OS = Homo sapiens OX = 9606 17 kDa 0 85 19 19 GN =RPS13 PE = 1 SV = 2 40S ribosomal protein S15a OS = Homo sapiens 11 kDa0 25 0 0 GN = RPS15A PE = 1 SV = 1 40S ribosomal protein S18 OS = Homosapiens 18 kDa 0 51 0 0 GN = RPS18 PE = 1 SV = 3 40S ribosomal proteinS28 OS = Homo sapiens  8 kDa 0 6 0 0 GN = RPS28 PE = 1 SV = 1 40Sribosomal protein S29 OS = Homo sapiens OX = 9606  7 kDa 0 16 1 1 GN =RPS29 PE = 1 SV = 2 40S ribosomal protein S3a OS = Homo sapiens 30 kDa 053 0 0 GN = RPS3A PE = 1 SV = 2 40S ribosomal protein S4, Y isoform 1 OS= Homo 29 kDa 0 0 0 9 sapiens OX = 9606 GN = RPS4Y1 PE = 1 SV = 2 40Sribosomal protein SA (Fragment) OS = Homo sapiens 29 kDa 0 8 0 0 GN =RPSA PE = 1 SV = 8 60S acidic ribosomal protein P2 OS = Homo sapiens 12kDa 0 50 0 0 GN = RPLP2 PE = 1 SV = 1 60S ribosomal protein L13a OS =Homo sapiens 24 kDa 0 33 0 0 GN = RPL13A PE = 1 SV = 2 60S ribosomalprotein L14 OS = Homo sapiens 23 kDa 0 34 0 0 GN = RPL14 PE = 1 SV = 460S ribosomal protein L18a OS = Homo sapiens 21 kDa 0 31 0 0 GN = RPL18APE = 1 SV = 2 60S ribosomal protein L23a (Fragment) OS = Homo 18 kDa 032 0 0 sapiens GN = RPL23A PE = 1 SV = 1 60S ribosomal protein L29 OS =Homo sapiens 18 kDa 0 6 0 0 GN = RPL29 PE = 1 SV = 2 60S ribosomalprotein L32 (Fragment) OS = Homo 16 kDa 0 20 0 0 sapiens GN = RPL32 PE =1 SV = 1 60S ribosomal protein L34 OS = Homo sapiens OX = 9606 13 kDa 00 3 3 GN = RPL34 PE = 1 SV = 3 60S ribosomal protein L35 OS = Homosapiens 15 kDa 0 24 0 0 GN = RPL35 PE = 1 SV = 2 60S ribosomal proteinL38 OS = Homo sapiens OX = 9606  8 kDa 0 0 5 4 GN = RPL38 PE = 1 SV = 278 kDa glucose-regulated protein OS = Homo sapiens 72 kDa 0 188 0 0 GN =HSPA5 PE = 1 SV = 2 A disintegrin and metalloproteinase withthrombospondin 105 kDa  0 12 3 0 motifs 1 OS = Homo sapiens OX = 9606 GN= ADAMTS1 PE = 1 SV = 4 Actin-related protein 10 OS = Homo sapiens 46kDa 0 3 0 0 GN = ACTR10 PE = 1 SV = 1 Actin-related protein 2/3 complexsubunit 1B OS = Homo 41 kDa 0 28 0 0 sapiens GN = ARPC1B PE = 1 SV = 3ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2 36 kDa 0 3 5 2 OS =Homo sapiens OX = 9606 GN = BST1 PE = 1 SV = 2 Aminopeptidase N OS =Homo sapiens GN = ANPEP 110 kDa  0 194 0 0 PE = 1 SV = 4Angiopoietin-related protein 4 OS = Homo sapiens 45 kDa 0 1 3 0 OX =9606 GN = ANGPTL4 PE = 1 SV = 2 Annexin A3 OS = Homo sapiens OX = 9606GN = ANXA3 36 kDa 0 0 0 3 PE = 1 SV = 3 Annexin A4 OS = Homo sapiens OX= 9606 GN = ANXA4 36 kDa 0 0 0 2 PE = 1 SV = 4 AP-2 complex subunitsigma OS = Homo sapiens 19 kDa 0 3 0 0 GN = AP2S1 PE = 1 SV = 1 Arf-GAPwith Rho-GAP domain, ANK repeat and PH 170 kDa  0 0 0 2domain-containing protein 3 OS = Homo sapiens OX = 9606 GN = ARAP3 PE =1 SV = 1 ATP synthase subunit epsilon-like protein, mitochondrial  6 kDa0 3 0 0 OS = Homo sapiens GN = ATP5EP2 PE = 3 SV = 1 ATP synthasesubunit g, mitochondrial OS = Homo 11 kDa 0 6 0 0 sapiens GN = ATP5L PE= 1 SV = 3 ATPase ASNA1 OS = Homo sapiens GN = ASNA1 PE = 1 39 kDa 0 2 00 SV = 2 ATPase, H+ transporting, lysosomal accessory protein 1, 32 kDa0 7 0 0 isoform CRA_c OS = Homo sapiens GN = ATP6AP1 PE = 1 SV = 1ATP-dependent DNA helicase Q1 OS = Homo sapiens 73 kDa 0 2 0 0 GN =RECQL PE = 1 SV = 3 Barrier-to-autointegration factor OS = Homo sapiens10 kDa 0 24 0 0 GN = BANF1 PE = 1 SV = 1 Basement membrane-specificheparan sulfate 469 kDa  0 4914 0 0 proteoglycan core protein OS = Homosapiens GN = HSPG2 PE = 1 SV = 4 Beta-catenin-like protein 1 OS = Homosapiens 65 kDa 0 2 0 0 GN = CTNNBL1 PE = 1 SV = 1 Beta-galactosidase OS= Homo sapiens GN = GLB1 PE = 1 76 kDa 0 3 0 0 SV = 2 Biglycan OS = Homosapiens OX = 9606 GN = BGN PE = 1 42 kDa 0 0 23 0 SV = 2 Bonemorphogenetic protein 1 OS = Homo sapiens 111 kDa  0 14 3 0 OX = 9606 GN= BMP1 PE = 1 SV = 2 Brain acid soluble protein 1 OS = Homo sapiens 23kDa 0 10 0 0 GN = BASP1 PE = 1 SV = 2 C-1-tetrahydrofolate synthase,cytoplasmic OS = Homo 102 kDa  0 2 0 0 sapiens GN = MTHFD1 PE = 1 SV = 3Calmodulin OS = Homo sapiens GN = CALM1 PE = 1 17 kDa 0 24 0 0 SV = 2Calpain small subunit 1 OS = Homo sapiens 34 kDa 0 34 0 0 GN = CAPNS1 PE= 1 SV = 1 Carboxypeptidase M OS = Homo sapiens GN = CPM PE = 1 51 kDa 05 0 0 SV = 2 Cardiomyopathy-associated protein 5 OS = Homo sapiens 449kDa  0 2 0 0 GN = CMYA5 PE = 1 SV = 3 Casein kinase II subunit alpha OS= Homo sapiens 45 kDa 0 10 1 1 OX = 9606 GN = CSNK2A1 PE = 1 SV = 1CD2-associated protein OS = Homo sapiens GN = CD2AP 71 kDa 0 9 0 0 PE =1 SV = 1 CD59 glycoprotein OS = Homo sapiens OX = 9606 14 kDa 0 0 3 0 GN= CD59 PE = 1 SV = 1 Chloride intracellular channel protein 4 OS = Homo29 kDa 0 2 0 0 sapiens GN = CLIC4 PE = 1 SV = 4 Chloride intracellularchannel protein 6 OS = Homo 73 kDa 0 2 0 0 sapiens GN = CLIC6 PE = 2 SV= 3 Chromobox protein homolog 1 (Fragment) OS = Homo 19 kDa 0 5 0 0sapiens GN = CBX1 PE = 1 SV = 1 Coiled-coil domain-containing protein124 OS = Homo 26 kDa 0 6 0 0 sapiens GN = CCDC124 PE = 1 SV = 1Coiled-coil domain-containing protein 30 OS = Homo 91 kDa 0 2 0 0sapiens GN = CCDC30 PE = 2 SV = 1 Coiled-coil-helix-coiled-coil-helixdomain-containing 13 kDa 0 2 0 0 protein 1 OS = Homo sapiens GN = CHCHD1PE = 1 SV = 1 Collagen alpha-1(II) chain OS = Homo sapiens OX = 9606 142kDa  0 2 1 1 GN = COL2A1 PE = 1 SV = 3 Collagen alpha-1(VI) chain OS =Homo sapiens 108 kDa  0 3 0 0 GN = COL6A1 PE = 1 SV = 1 Collagenalpha-1(X) chain OS = Homo sapiens 66 kDa 0 3 0 0 GN = COL10A1 PE = 1 SV= 2 Collagen alpha-1(XXIII) chain OS = Homo sapiens 52 kDa 0 2 0 0 GN =COL23A1 PE = 1 SV = 1 Collagen alpha-1(XXVII) chain OS = Homo sapiens187 kDa  0 1 0 2 OX = 9606 GN = COL27A1 PE = 1 SV = 1 Collagenalpha-2(IX) chain OS = Homo sapiens OX = 9606 65 kDa 0 0 0 0 GN = COL9A2PE = 1 SV = 2 Collagen triple helix repeat-containing protein 1 26 kDa 03 1 1 OS = Homo sapiens OX = 9606 GN = CTHRC1 PE = 1 SV = 1 Connectivetissue growth factor OS = Homo sapiens 38 kDa 0 4 6 2 OX = 9606 GN =CTGF PE = 1 SV = 2 COP9 signalosome complex subunit 5 OS = Homo sapiens38 kDa 0 2 0 0 GN = COPS5 PE = 1 SV = 4 Coronin-1B OS = Homo sapiens GN= CORO1B PE = 1 54 kDa 0 9 0 0 SV = 1 Cullin-1 OS = Homo sapiens GN =CUL1 PE = 1 SV = 2 90 kDa 0 3 0 0 Cytochrome c (Fragment) OS = Homosapiens GN = CYCS 11 kDa 0 3 0 0 PE = 1 SV = 1 Cytochrome c oxidasesubunit 4 isoform 1, mitochondrial 20 kDa 0 5 0 0 OS = Homo sapiens GN =COX4I1 PE = 1 SV = 1 Cytoplasmic aconitate hydratase OS = Homo sapiens98 kDa 0 0 0 2 OX = 9606 GN = ACO1 PE = 1 SV = 3 Cytosolic non-specificdipeptidase OS = Homo sapiens 53 kDa 0 10 0 8 OX = 9606 GN = CNDP2 PE =1 SV = 2 Death-associated protein kinase 3 OS = Homo sapiens 53 kDa 0 60 0 OX = 9606 GN = DAPK3 PE = 1 SV = 1 Deoxyribose-phosphate aldolase OS= Homo sapiens 35 kDa 0 0 0 3 OX = 9606 GN = DERA PE = 1 SV = 2Desmoglein-1 OS = Homo sapiens GN = DSG1 PE = 1 114 kDa  0 6 0 0 SV = 2DNA damage-binding protein 1 OS = Homo sapiens 127 kDa  0 14 0 3 OX =9606 GN = DDB1 PE = 1 SV = 1 DNA topoisomerase 1 OS = Homo sapiens GN =TOP1 91 kDa 0 10 0 0 PE = 1 SV = 2 DNA-directed RNA polymerase IIsubunit RPB1 217 kDa  0 11 0 0 OS = Homo sapiens OX = 9606 GN = POLR2APE = 1 SV = 2 DNA-directed RNA polymerase II subunit RPB2 134 kDa  0 140 0 OS = Homo sapiens GN = POLR2B PE = 1 SV = 1 DNA-directed RNApolymerase II subunit RPB3 31 kDa 0 9 0 0 OS = Homo sapiens GN = POLR2CPE = 1 SV = 2 DNA-directed RNA polymerase II subunit RPB4 13 kDa 0 6 0 0OS = Homo sapiens GN = POLR2D PE = 1 SV = 1 DNA-directed RNA polymerasesI, II, and III subunit 17 kDa 0 2 3 0 RPABC3 OS = Homo sapiens OX = 9606GN = POLR2H PE = 1 SV = 4 DnaJ homolog subfamily B member 11 OS = Homo41 kDa 0 0 0 4 sapiens OX = 9606 GN = DNAJB11 PE = 1 SV = 1Dolichol-phosphate mannosyltransferase subunit 1 30 kDa 0 2 0 0 OS =Homo sapiens GN = DPM1 PE = 1 SV = 1Dolichyl-diphosphooligosaccharide--protein 94 kDa 0 2 0 0glycosyltransferase subunit STT3B OS = Homo sapiens GN = STT3B PE = 1 SV= 1 Doublecortin domain-containing protein 2 OS = Homo 53 kDa 0 17 0 2sapiens OX = 9606 GN = DCDC2 PE = 1 SV = 2 Double-stranded RNA-bindingprotein Staufen homolog 63 kDa 0 15 4 4 1 OS = Homo sapiens OX = 9606 GN= STAU1 PE = 1 SV = 2 Dynactin subunit 2 OS = Homo sapiens GN = DCTN2 44kDa 0 6 0 2 PE = 1 SV = 4 Dynein heavy chain 10, axonemal OS = Homosapiens 515 kDa  0 1 0 2 OX = 9606 GN = DNAH10 PE = 1 SV = 4 EBNA1binding protein 2, isoform CRA_d OS = Homo 41 kDa 0 5 0 0 sapiens GN =EBNA1BP2 PE = 1 SV = 1 Ectonucleotide pyrophosphatase/phosphodiesterase105 kDa  0 0 0 2 family member 1 OS = Homo sapiens OX = 9606 GN = ENPP1PE = 1 SV = 2 EH domain-containing protein 4 OS = Homo sapiens 61 kDa 05 0 3 OX = 9606 GN = EHD4 PE = 1 SV = 1 Elongation factor Tu,mitochondrial OS = Homo sapiens 50 kDa 0 9 0 0 GN = TUFM PE = 1 SV = 2Emerin OS = Homo sapiens GN = EMD PE = 1 SV = 1 29 kDa 0 6 0 0 Epoxidehydrolase 1 OS = Homo sapiens OX = 9606 53 kDa 0 0 0 3 GN = EPHX1 PE = 1SV = 1 ER lumen protein-retaining receptor 3 OS = Homo sapiens 25 kDa 05 0 0 GN = KDELR3 PE = 2 SV = 1 Eukaryotic translation initiation factor3 subunit E 52 kDa 0 8 0 0 OS = Homo sapiens GN = EIF3E PE = 1 SV = 1Eukaryotic translation initiation factor 3 subunit F 38 kDa 0 14 0 0 OS= Homo sapiens GN = EIF3F PE = 1 SV = 1 Eukaryotic translationinitiation factor 3 subunit H 40 kDa 0 5 0 0 OS = Homo sapiens GN =EIF3H PE = 1 SV = 1 FACT complex subunit SPT16 OS = Homo sapiens 120kDa  0 7 0 0 GN = SUPT16H PE = 1 SV = 1 Far upstream element-bindingprotein 1 OS = Homo 68 kDa 0 3 0 1 sapiens OX = 9606 GN = FUBP1 PE = 1SV = 3 Fascin OS = Homo sapiens GN = FSCN1 PE = 1 SV = 3 55 kDa 0 12 0 0Fibrous sheath-interacting protein 2 OS = Homo sapiens 781 kDa  0 0 2 1OX = 9606 GN = FSIP2 PE = 2 SV = 4 Filaggrin OS = Homo sapiens GN = FLGPE = 1 SV = 3 435 kDa  0 3 0 0 Filaggrin-2 OS = Homo sapiens GN = FLG2PE = 1 SV = 1 248 kDa  0 2 0 0 Flotillin-2 OS = Homo sapiens GN = FLOT2PE = 1 SV = 1 53 kDa 0 13 0 0 Galectin-3 OS = Homo sapiens GN = LGALS3PE = 1 SV = 5 26 kDa 0 39 0 0 Galectin-3-binding protein OS = Homosapiens OX = 9606 65 kDa 0 4 0 3 GN = LGALS3BP PE = 1 SV = 1 Galectin-8OS = Homo sapiens OX = 9606 GN = LGALS8 36 kDa 0 5 2 0 PE = 1 SV = 4Glutamate dehydrogenase 1, mitochondrial OS = Homo 61 kDa 0 4 0 0sapiens OX = 9606 GN = GLUD1 PE = 1 SV = 2 Glutathione peroxidase 1 OS =Homo sapiens GN = GPX1 22 kDa 0 3 0 2 PE = 1 SV = 4 GlycylpeptideN-tetradecanoyltransferase 2 OS = Homo 57 kDa 0 2 0 0 sapiens GN = NMT2PE = 1 SV = 1 Glypican-6 OS = Homo sapiens GN = GPC6 PE = 1 SV = 1 63kDa 0 14 0 0 Golgi-associated plant pathogenesis-related protein 1 17kDa 0 20 0 0 OS = Homo sapiens GN = GLIPR2 PE = 1 SV = 3 Gremlin-1 OS =Homo sapiens OX = 9606 GN = GREM1 21 kDa 0 0 3 0 PE = 1 SV = 1 Guaninenucleotide-binding protein G(I)/G(S)/G(O)  8 kDa 0 6 0 0 subunitgamma-12 OS = Homo sapiens GN = GNG12 PE = 1 SV = 3 Guaninenucleotide-binding protein G(k) subunit alpha 41 kDa 0 17 0 0 OS = Homosapiens GN = GNAI3 PE = 1 SV = 3 Hamartin OS = Homo sapiens GN = TSC1 PE= 1 SV = 2 130 kDa  0 0 0 2 Heat shock-related 70 kDa protein 2 OS =Homo sapiens 70 kDa 0 0 0 24 OX = 9606 GN = HSPA2 PE = 1 SV = 1Hemicentin-2 OS = Homo sapiens OX = 9606 542 kDa  0 0 0 0 GN = HMCN2 PE= 2 SV = 3 Heparan sulfate glucosamine 3-O-sulfotransferase 6 37 kDa 0 30 0 OS = Homo sapiens GN = HS3ST6 PE = 1 SV = 2 Heterogeneous nuclearribonucleoprotein A0 OS = Homo 31 kDa 0 15 0 0 sapiens GN = HNRNPA0 PE =1 SV = 1 Heterogeneous nuclear ribonucleoprotein H2 OS = Homo 49 kDa 014 0 0 sapiens GN = HNRNPH2 PE = 1 SV = 1 Homeobox protein Hox-B3 OS =Homo sapiens OX = 9606 44 kDa 0 5 0 0 GN = HOXB3 PE = 2 SV = 2 HornerinOS = Homo sapiens GN = HRNR PE = 1 SV = 2 282 kDa  0 15 0 0Hsc70-interacting protein (Fragment) OS = Homo sapiens 16 kDa 0 2 0 0 GN= ST13 PE = 1 SV = 1 Hyaluronan and proteoglycan link protein 1 OS =Homo 40 kDa 0 0 0 0 sapiens OX = 9606 GN = HAPLN1 PE = 2 SV = 2Hyaluronan and proteoglycan link protein 3 OS = Homo 41 kDa 0 6 0 0sapiens GN = HAPLN3 PE = 2 SV = 1 Intercellular adhesion molecule 1 OS =Homo sapiens 58 kDa 0 16 0 0 GN = ICAM1 PE = 1 SV = 2 Interleukinenhancer-binding factor 2 OS = Homo sapiens 39 kDa 0 29 0 0 GN = ILF2 PE= 1 SV = 1 Isocitrate dehydrogenase [NADP] cytoplasmic 47 kDa 0 7 0 0 OS= Homo sapiens GN = IDH1 PE = 1 SV = 2 Isoform 1 of Gamma-adducin OS =Homo sapiens 76 kDa 0 7 0 1 OX = 9606 GN = ADD3 Isoform 1 ofPolypyrimidine tract-binding protein 3 57 kDa 0 5 0 3 OS = Homo sapiensOX = 9606 GN = PTBP3 Isoform 1 of Synaptic functional regulator FMR1 67kDa 0 5 0 1 OS = Homo sapiens OX = 9606 GN = FMR1 Isoform 2 of2,4-dienoyl-CoA reductase, mitochondrial 35 kDa 0 8 0 1 OS = Homosapiens OX = 9606 GN = DECR1 Isoform 2 of A-kinase anchor protein 13 OS= Homo 308 kDa  0 2 0 0 sapiens OX = 9606 GN = AKAP13 Isoform 2 ofAnkyrin repeat domain-containing protein 274 kDa  0 0 0 0 17 OS = Homosapiens OX = 9606 GN = ANKRD17 Isoform 2 of AP-2 complex subunit mu OS =Homo 49 kDa 0 9 0 1 sapiens OX = 9606 GN = AP2M1 Isoform 2 ofBcl-2-associated transcription factor 1 106 kDa  0 4 0 0 OS = Homosapiens OX = 9606 GN = BCLAF1 Isoform 2 of Cadherin-2 OS = Homo sapiensOX = 9606 97 kDa 0 7 0 2 GN = CDH2 Isoform 2 of Calponin-1 OS = Homosapiens OX = 9606 31 kDa 0 1 5 8 GN = CNN1 Isoform 2 of ChromodomainY-like protein OS = Homo 61 kDa 0 4 0 0 sapiens GN = CDYL Isoform 2 ofCollagen alpha-1(VII) chain OS = Homo 292 kDa  0 70 2 1 sapiens OX =9606 GN = COL7A1 Isoform 2 of Cyclin-Y OS = Homo sapiens GN = CCNY 37kDa 0 3 0 0 Isoform 2 of Cysteine-rich protein 2 OS = Homo sapiens 30kDa 0 5 0 0 OX = 9606 GN = CRIP2 Isoform 2 of DnaJ homolog subfamily Cmember 10 86 kDa 0 4 0 0 OS = Homo sapiens GN = DNAJC10 Isoform 2 ofFibroblast growth factor 2 OS = Homo 23 kDa 0 13 3 1 sapiens OX = 9606GN = FGF2 Isoform 2 of Fibulin-2 OS = Homo sapiens OX = 9606 132 kDa  06 0 0 GN = FBLN2 Isoform 2 of GDNF family receptor alpha-1 OS = Homo 51kDa 0 8 0 0 sapiens GN = GFRA1 Isoform 2 of Glycogen phosphorylase,liver form 93 kDa 0 2 0 1 OS = Homo sapiens OX = 9606 GN = PYGL Isoform2 of Golgin subfamily A member 5 OS = Homo 78 kDa 0 0 0 0 sapiens OX =9606 GN = GOLGA5 Isoform 2 of H/ACA ribonucleoprotein complex subunit 21kDa 0 1 1 2 1 OS = Homo sapiens OX = 9606 GN = GAR1 Isoform 2 ofHelicase SRCAP OS = Homo sapiens 337 kDa  0 2 0 0 OX = 9606 GN = SRCAPIsoform 2 of Histone-binding protein RBBP4 OS = Homo 48 kDa 0 6 0 0sapiens OX = 9606 GN = RBBP4 Isoform 2 of Histone-lysineN-methyltransferase, H3 267 kDa  0 6 0 1 lysine-36 and H4 lysine-20specific OS = Homo sapiens OX = 9606 GN = NSD1 Isoform 2 of Insulin-likegrowth factor-binding protein 7 29 kDa 0 54 13 7 OS = Homo sapiens OX =9606 GN = IGFBP7 Isoform 2 of Interferon-inducible double-stranded RNA-33 kDa 0 4 1 1 dependent protein kinase activator A OS = Homo sapiens OX= 9606 GN = PRKRA Isoform 2 of KH domain-containing, RNA-binding, 30 kDa0 8 0 0 signal transduction-associated protein 3 OS = Homo sapiens GN =KHDRBS3 Isoform 2 of Lactadherin OS = Homo sapiens 35 kDa 0 25 2 1 GN =MFGE8 Isoform 2 of L-amino-acid oxidase OS = Homo sapiens 65 kDa 0 2 0 1OX = 9606 GN = IL4I1 Isoform 2 of Macrophage-capping protein OS = Homo37 kDa 0 3 0 0 sapiens GN = CAPG Isoform 2 of Matrilin-2 OS = Homosapiens OX = 9606 105 kDa  0 37 8 5 GN = MATN2 Isoform 2 ofMesoderm-specific transcript homolog 38 kDa 0 0 0 4 protein OS = Homosapiens OX = 9606 GN = MEST Isoform 2 of Microtubule-associated protein1A 306 kDa  0 4 2 3 OS = Homo sapiens OX = 9606 GN = MAP1A Isoform 2 ofMidkine OS = Homo sapiens OX = 9606 10 kDa 0 4 2 2 GN = MDK Isoform 2 ofMonoacylglycerol lipase ABHD12 46 kDa 0 2 0 0 OS = Homo sapiens GN =ABHD12 Isoform 2 of Multidrug resistance protein 1 OS = Homo 134 kDa  01 0 2 sapiens OX = 9606 GN = ABCB1 Isoform 2 of Myosin-11 OS = Homosapiens GN = MYH11 228 kDa  0 161 37 0 Isoform 2 of Myosin-14 OS = Homosapiens OX = 9606 232 kDa  0 141 26 26 GN = MYH14 Isoform 2 of NADHdehydrogenase [ubiquinone] 1 beta 14 kDa 0 1 0 2 subcomplex subunit 4 OS= Homo sapiens OX = 9606 GN = NDUFB4 Isoform 2 of Nuclear receptorcorepressor 1 OS = Homo 259 kDa  0 0 2 0 sapiens OX = 9606 GN = NCOR1Isoform 2 of Periostin OS = Homo sapiens OX = 9606 87 kDa 0 0 5 5 GN =POSTN Isoform 2 of Pescadillo homolog OS = Homo sapiens 67 kDa 0 5 1 0OX = 9606 GN = PES1 Isoform 2 of Platelet-derived growth factor subunitB 26 kDa 0 1 3 1 OS = Homo sapiens OX = 9606 GN = PDGFB Isoform 2 ofProtein ELYS OS = Homo sapiens OX = 9606 256 kDa  0 0 2 0 GN = AHCTF1Isoform 2 of Ran-binding protein 3 OS = Homo sapiens 60 kDa 0 5 0 0 OX =9606 GN = RANBP3 Isoform 2 of Regulator of chromosome condensation 48kDa 0 16 0 0 OS = Homo sapiens OX = 9606 GN = RCC1 Isoform 2 ofRetinol-binding protein 1 OS = Homo 17 kDa 0 0 0 2 sapiens OX = 9606 GN= RBP1 Isoform 2 of RNA-binding protein 39 OS = Homo sapiens 59 kDa 0 00 2 GN = RBM39 Isoform 2 of RNA-binding protein 8A OS = Homo sapiens 20kDa 0 5 0 1 OX = 9606 GN = RBM8A Isoform 2 of Ryanodine receptor 1 OS =Homo sapiens 565 kDa  0 1 0 2 OX = 9606 GN = RYR1 Isoform 2 ofSCO-spondin OS = Homo sapiens OX = 9606 139 kDa  0 3 0 0 GN = SSPOIsoform 2 of Semaphorin-7A OS = Homo sapiens 73 kDa 0 3 1 3 OX = 9606 GN= SEMA7A Isoform 2 of Septin-8 OS = Homo sapiens OX = 9606 50 kDa 0 12 03 GN = SEPT8 Isoform 2 of Serine/threonine-protein phosphatase 28 kDa 05 0 1 PGAM5, mitochondrial OS = Homo sapiens OX = 9606 GN = PGAM5Isoform 2 of SH3 domain-containing kinase-binding 69 kDa 0 2 0 2 protein1 OS = Homo sapiens OX = 9606 GN = SH3KBP1 Isoform 2 of Signalrecognition particle receptor subunit 67 kDa 0 3 0 1 alpha OS = Homosapiens OX = 9606 GN = SRPRA Isoform 2 of Signal-inducedproliferation-associated 1- 197 kDa  0 0 0 0 like protein 1 OS = Homosapiens OX = 9606 GN = SIPA1L1 Isoform 2 of Sorting nexin-27 OS = Homosapiens 60 kDa 0 4 0 0 GN = SNX27 Isoform 2 of Spectrin beta chain,erythrocytic OS = Homo 268 kDa  0 8 0 2 sapiens OX = 9606 GN = SPTBIsoform 2 of Testis-expressed protein 10 OS = Homo 104 kDa 0 2 0 0sapiens OX = 9606 GN = TEX10 Isoform 2 of Tissue factor pathwayinhibitor 2 OS = Homo 26 kDa 0 9 6 0 sapiens OX = 9606 GN = TFPI2Isoform 2 of Tyrosine-protein kinase BAZ1B OS = Homo 170 kDa  0 4 0 0sapiens OX = 9606 GN = BAZ1B Isoform 2 of UDP-glucuronosyltransferase1-6 30 kDa 0 4 0 0 OS = Homo sapiens OX = 9606 GN = UGT1A6 Isoform 2 ofVesicle-associated membrane protein- 33 kDa 0 3 0 1 associated protein AOS = Homo sapiens OX = 9606 GN = VAPA Isoform 2 of Voltage-dependentcalcium channel subunit 123 kDa  0 2 1 0 alpha-2/delta-1 OS = Homosapiens OX = 9606 GN = CACNA2D1 Isoform 2 of Y-box-binding protein 3 OS= Homo sapiens 32 kDa 0 15 0 1 OX = 9606 GN = YBX3 Isoform 2 of Zincfinger homeobox protein 4 OS = Homo 397 kDa  0 2 0 0 sapiens OX = 9606GN = ZFHX4 Isoform 3 of 1-phosphatidylinositol 4,5-bisphosphate 136 kDa 0 3 0 0 phosphodiesterase beta-4 OS = Homo sapiens OX = 9606 GN = PLCB4Isoform 3 of Alpha-adducin OS = Homo sapiens 84 kDa 0 2 0 0 GN = ADD1Isoform 3 of Cytoskeleton-associated protein 5 226 kDa  0 3 0 0 OS =Homo sapiens OX = 9606 GN = CKAP5 Isoform 3 of DnaJ homolog subfamily Cmember 11 57 kDa 0 2 0 1 OS = Homo sapiens GN = DNAJC11 Isoform 3 of E3ubiquitin-protein ligase CHFR 69 kDa 0 0 0 0 OS = Homo sapiens OX = 9606GN = CHFR Isoform 3 of H/ACA ribonucleoprotein complex subunit 48 kDa 013 1 1 DKC1 OS = Homo sapiens OX = 9606 GN = DKC1 Isoform 3 ofHistone-lysine N-methyltransferase 2D 594 kDa  0 1 0 1 OS = Homo sapiensOX = 9606 GN = KMT2D Isoform 3 of Latent-transforming growth factorbeta- 169 kDa  0 34 0 0 binding protein 4 OS = Homo sapiens OX = 9606 GN= LTBP4 Isoform 3 of Malate dehydrogenase, cytoplasmic 39 kDa 0 3 0 0 OS= Homo sapiens OX = 9606 GN = MDH1 Isoform 3 of Putative oxidoreductaseGLYR1 OS = Homo 60 kDa 0 4 0 0 sapiens OX = 9606 GN = GLYR1 Isoform 3 ofScaffold attachment factor B1 OS = Homo 103 kDa  0 3 0 0 sapiens GN =SAFB Isoform 3 of Torsin-1A-interacting protein 1 OS = Homo 66 kDa 0 5 00 sapiens GN = TOR1AIP1 Isoform 4 of CD109 antigen OS = Homo sapiens 160kDa  0 18 0 0 GN = CD109 Isoform 4 of FYVE and coiled-coildomain-containing 169 kDa  0 0 0 0 protein 1 OS = Homo sapiens OX = 9606GN = FYCO1 Isoform 4 of IQ domain-containing protein N OS = Homo 147kDa  0 0 0 3 sapiens OX = 9606 GN = IQCN Isoform 4 of Kinesin-likeprotein KIF24 OS = Homo 129 kDa  0 2 0 0 sapiens OX = 9606 GN = KIF24Isoform 4 of Latent-transforming growth factor beta- 187 kDa  0 1 4 1binding protein 1 OS = Homo sapiens OX = 9606 GN = LTBP1 Isoform 4 ofProtein diaphanous homolog 3 OS = Homo 136 kDa  0 2 0 0 sapiens GN =DIAPH3 Isoform 5 of E1A-binding protein p400 OS = Homo 340 kDa  0 1 0 0sapiens OX = 9606 GN = EP400 Isoform 5 of Immunoglobulin-like andfibronectin type 384 kDa  0 3 0 2 III domain-containing protein 1 OS =Homo sapiens OX = 9606 GN = IGFN1 Isoform 5 of LIM domain only protein 7OS = Homo 158 kDa  0 8 0 0 sapiens GN = LMO7 Isoform 5 of Papilin OS =Homo sapiens OX = 9606 136 kDa  0 17 1 1 GN = PAPLN Isoform 6 of Treacleprotein OS = Homo sapiens 148 kDa  0 2 0 0 OX = 9606 GN = TCOF1 IsoformB of Collagen alpha-1(XI) chain OS = Homo 182 kDa  0 0 1 2 sapiens OX =9606 GN = COL11A1 Isoform B of Collagen alpha-6(IV) chain OS = Homo 164kDa  0 5 2 0 sapiens OX = 9606 GN = COL4A6 Isoform B of DnaJ homologsubfamily B member 6 27 kDa 0 0 1 2 OS = Homo sapiens OX = 9606 GN =DNAJB6 Isoform B of Methyl-CpG-binding protein 2 OS = Homo 53 kDa 0 11 10 sapiens OX = 9606 GN = MECP2 Isoform B of Ras-related C3 botulinumtoxin substrate 1 23 kDa 0 3 0 0 OS = Homo sapiens OX = 9606 GN = RAC1Isoform B of Transforming growth factor beta-2 51 kDa 0 5 1 2 proproteinOS = Homo sapiens OX = 9606 GN = TGFB2 Isoform Beta-3B of Integrinbeta-3 OS = Homo sapiens 86 kDa 0 4 0 2 OX = 9606 GN = ITGB3 Isoform Cof Fibulin-1 OS = Homo sapiens OX = 9606 74 kDa 0 13 0 8 GN = FBLN1Isoform Long of Proteasome subunit alpha type-1 30 kDa 0 2 0 2 OS = Homosapiens OX = 9606 GN = PSMA1 Isoform Non-brain of Clathrin light chain AOS = Homo 24 kDa 0 17 0 2 sapiens OX = 9606 GN = CLTA Isoform Short ofLaminin subunit gamma-2 OS = Homo 122 kDa  0 5 0 1 sapiens OX = 9606 GN= LAMC2 Keratin, type I cytoskeletal 14 OS = Homo sapiens 52 kDa 0 94 018 OX = 9606 GN = KRT14 PE = 1 SV = 4 Keratin, type I cytoskeletal 16 OS= Homo sapiens 51 kDa 0 99 0 0 GN = KRT16 PE = 1 SV = 4 Keratin, type Icytoskeletal 19 OS = Homo sapiens 44 kDa 0 753 0 0 GN = KRT19 PE = 1 SV= 4 Keratin, type II cuticular Hb5 OS = Homo sapiens 56 kDa 0 3 0 0 GN =KRT85 PE = 1 SV = 1 Keratin, type II cytoskeletal 1 OS = Homo sapiens 66kDa 0 497 0 0 GN = KRT1 PE = 1 SV = 6 Keratin, type II cytoskeletal 5 OS= Homo sapiens 62 kDa 0 93 0 0 GN = KRT5 PE = 1 SV = 3 Keratin, type IIcytoskeletal 6B OS = Homo sapiens 60 kDa 0 104 18 0 OX = 9606 GN = KRT6BPE = 1 SV = 5 Keratin, type II cytoskeletal 7 OS = Homo sapiens 51 kDa 00 0 135 OX = 9606 GN = KRT7 PE = 1 SV = 5 Kinesin-like protein KIFC2 OS= Homo sapiens OX = 9606 90 kDa 0 2 0 1 GN = KIFC2 PE = 2 SV = 1Ladinin-1 OS = Homo sapiens OX = 9606 GN = LAD1 57 kDa 0 0 0 6 PE = 1 SV= 2 Laminin subunit beta-3 OS = Homo sapiens GN = LAMB3 130 kDa  0 10 00 PE = 1 SV = 1 Latent-transforming growth factor beta-binding protein 2190 kDa  0 23 0 0 OS = Homo sapiens GN = LTBP2 PE = 1 SV = 1 Leucinezipper protein 1 OS = Homo sapiens OX = 9606 120 kDa  0 5 0 0 GN = LUZP1PE = 1 SV = 2 Lipoamide acyltransferase component of branched-chain 53kDa 0 6 4 8 alpha-keto acid dehydrogenase complex, mitochondrial OS =Homo sapiens OX = 9606 GN = DBT PE = 1 SV = 3 Low-density lipoproteinreceptor-related protein 1B 515 kDa  0 2 0 0 OS = Homo sapiens GN =LRP1B PE = 1 SV = 2 Low-density lipoprotein receptor-related protein 2522 kDa  0 0 0 2 OS = Homo sapiens OX = 9606 GN = LRP2 PE = 1 SV = 3Lysyl oxidase homolog 1 OS = Homo sapiens OX = 9606 63 kDa 0 24 1 0 GN =LOXL1 PE = 1 SV = 2 Magnesium transporter protein 1 OS = Homo sapiens 42kDa 0 4 1 1 GN = MAGT1 PE = 1 SV = 1 MARCKS-related protein OS = Homosapiens 20 kDa 0 4 0 0 GN = MARCKSL1 PE = 1 SV = 2 Metastasis-associatedprotein MTA2 OS = Homo sapiens 75 kDa 0 5 0 0 GN = MTA2 PE = 1 SV = 1Microsomal glutathione S-transferase 1 OS = Homo 18 kDa 0 0 0 3 sapiensOX = 9606 GN = MGST1 PE = 1 SV = 1 Mitochondrial GTPase 1 OS = Homosapiens OX = 9606 37 kDa 0 0 0 2 PE = 3 SV = 1 MKI67 FHAdomain-interacting nucleolar 20 kDa 0 6 0 0 phosphoprotein (Fragment) OS= Homo sapiens GN = NIFK PE = 1 SV = 1 Mucin-16 OS = Homo sapiens OX =9606 GN = MUC16 1519 kDa  0 0 2 4 PE = 1 SV = 3 Myelin expression factor2 OS = Homo sapiens OX = 9606 64 kDa 0 2 0 0 GN = MYEF2 PE = 1 SV = 3Myosin light chain 6B OS = Homo sapiens OX = 9606 23 kDa 0 0 6 0 GN =MYL6B PE = 1 SV = 1 Myristoylated alanine-rich C-kinase substrate OS =Homo 32 kDa 0 11 0 0 sapiens GN = MARCKS PE = 1 SV = 4 N-acylneuraminatecytidylyltransferase OS = Homo 48 kDa 0 6 0 0 sapiens GN = CMAS PE = 1SV = 2 NAD(P) transhydrogenase, mitochondrial OS = Homo 100 kDa  0 3 0 0sapiens GN = NNT PE = 1 SV = 1 Nestin OS = Homo sapiens GN = NES PE = 1SV = 2 177 kDa  0 18 0 0 Neurabin-2 OS = Homo sapiens GN = PPP1R9B PE =1 89 kDa 0 3 0 0 SV = 1 Neurobeachin OS = Homo sapiens OX = 9606 GN =NBEA 328 kDa  0 0 0 3 PE = 1 SV = 3 Nicotinate-nucleotidepyrophosphorylase [carboxylating] 31 kDa 0 0 0 4 OS = Homo sapiens OX =9606 GN = QPRT PE = 1 SV = 3 Non-syndromic hearing impairment protein 5OS = Homo 55 kDa 0 2 0 0 sapiens GN = DFNA5 PE = 1 SV = 2 Nuclearreceptor-binding protein OS = Homo sapiens 61 kDa 0 5 0 0 GN = NRBP1 PE= 1 SV = 1 Nucleolar complex protein 3 homolog OS = Homo sapiens 93 kDa0 5 0 0 GN = NOC3L PE = 1 SV = 1 Nucleolar complex protein 4 homolog OS= Homo sapiens 58 kDa 0 5 0 0 GN = NOC4L PE = 1 SV = 1 Nucleolar protein58 OS = Homo sapiens GN = NOP58 60 kDa 0 6 0 0 PE = 1 SV = 1 Nucleolartranscription factor 1 OS = Homo sapiens 87 kDa 0 2 0 0 GN = UBTF PE = 1SV = 1 Nucleoplasmin-3 OS = Homo sapiens GN = NPM3 PE = 1 19 kDa 0 3 0 0SV = 3 Palladin OS = Homo sapiens OX = 9606 GN = PALLD 151 kDa  0 7 0 6PE = 1 SV = 3 PDZ and LIM domain protein 1 OS = Homo sapiens 36 kDa 0 00 4 OX = 9606 GN = PDLIM1 PE = 1 SV = 4 PDZ and LIM domain protein 4 OS= Homo sapiens 35 kDa 0 3 0 0 GN = PDLIM4 PE = 1 SV = 2Pentraxin-related protein PTX3 OS = Homo sapiens 42 kDa 0 23 0 0 GN =PTX3 PE = 1 SV = 3 Peptidyl-prolyl cis-trans isomerase B OS = Homosapiens 24 kDa 0 52 0 0 GN = PPIB PE = 1 SV = 2 Peptidyl-prolylcis-trans isomerase FKBP10 OS = Homo 64 kDa 0 3 0 0 sapiens GN = FKBP10PE = 1 SV = 1 Peptidyl-prolyl cis-trans isomerase FKBP3 OS = Homo 25 kDa0 8 0 0 sapiens GN = FKBP3 PE = 1 SV = 1 Periaxin OS = Homo sapiens OX =9606 GN = PRX PE = 1 155 kDa  0 0 0 2 SV = 2 Periodic tryptophan protein1 homolog OS = Homo 56 kDa 0 2 0 0 sapiens OX = 9606 GN = PWP1 PE = 1 SV= 1 Peroxiredoxin-1 (Fragment) OS = Homo sapiens 19 kDa 0 13 0 0 GN =PRDX1 PE = 1 SV = 1 Phosphoglycerate mutase 1 OS = Homo sapiens 29 kDa 011 0 0 GN = PGAM1 PE = 1 SV = 2 Pinin OS = Homo sapiens OX = 9606 GN =PNN PE = 1 82 kDa 0 6 2 4 SV = 5 Platelet-activating factoracetylhydrolase IB subunit 47 kDa 0 4 0 0 alpha OS = Homo sapiens OX =9606 GN = PAFAH1B1 PE = 1 SV = 2 Poly [ADP-ribose] polymerase 1 OS =Homo sapiens 113 kDa  0 30 0 0 GN = PARP1 PE = 1 SV = 4Poly(U)-binding-splicing factor PUF60 (Fragment) 57 kDa 0 3 0 0 OS =Homo sapiens GN = PUF60 PE = 1 SV = 1 Polymerase delta-interactingprotein 3 OS = Homo sapiens 48 kDa 0 6 0 0 GN = POLDIP3 PE = 1 SV = 1Polymerase I and transcript release factor OS = Homo 43 kDa 0 89 0 0sapiens GN = PTRF PE = 1 SV = 1 Polyubiquitin-B OS = Homo sapiens GN =UBB PE = 1 17 kDa 0 111 0 0 SV = 1 POU domain, class 3, transcriptionfactor 3 OS = Homo 50 kDa 0 20 0 0 sapiens GN = POU3F3 PE = 2 SV = 2 PRdomain zinc finger protein 8 OS = Homo sapiens 72 kDa 0 2 0 0 GN = PRDM8PE = 1 SV = 3 Prefoldin subunit 6 OS = Homo sapiens GN = PFDN6 15 kDa 03 0 0 PE = 1 SV = 1 Probable ATP-dependent RNA helicase DDX27 87 kDa 0 40 0 OS = Homo sapiens GN = DDX27 PE = 1 SV = 1 Probable globaltranscription activator SNF2L1 123 kDa  0 6 0 0 OS = Homo sapiens OX =9606 GN = SMARCA1 PE = 1 SV = 2 Probable maltase-glucoamylase 2 OS =Homo sapiens 278 kDa  0 0 0 2 OX = 9606 GN = MGAM2 PE = 2 SV = 3Procollagen galactosyltransferase 1 OS = Homo sapiens 72 kDa 0 3 0 0 GN= COLGALT1 PE = 1 SV = 1 Procollagen-lysine, 2-oxoglutarate5-dioxygenase 3 85 kDa 0 3 0 0 OS = Homo sapiens GN = PLOD3 PE = 1 SV =1 Prolow-density lipoprotein receptor-related protein 1 505 kDa 0 2 0 0OS = Homo sapiens GN = LRP1 PE = 1 SV = 2 Protein disulfide-isomerase OS= Homo sapiens 53 kDa 0 30 0 0 GN = P4HB PE = 1 SV = 2 Protein GREB1 OS= Homo sapiens GN = GREB1 PE = 2 216 kDa  0 0 0 2 SV = 1 Protein kinaseC delta-binding protein OS = Homo sapiens 31 kDa 0 20 0 0 GN = PRKCDBPPE = 1 SV = 1 Protein MAK16 homolog OS = Homo sapiens 35 kDa 0 2 0 0 GN= MAK16 PE = 1 SV = 2 Protein S100-A10 OS = Homo sapiens GN = S100A10 11kDa 0 11 0 0 PE = 1 SV = 2 Protein S100-A13 OS = Homo sapiens GN =S100A13 11 kDa 0 5 0 0 PE = 1 SV = 1 Protein S100-A9 OS = Homo sapiensGN = S100A9 PE = 1 13 kDa 0 12 0 0 SV = 1 Protein-glutaminegamma-glutamyltransferase E 77 kDa 0 2 0 0 OS = Homo sapiens GN = TGM3PE = 1 SV = 4 Pumilio homolog 3 OS = Homo sapiens GN = PUM3 PE = 1 74kDa 0 6 0 0 SV = 3 Raftlin OS = Homo sapiens GN = RFTN1 PE = 1 SV = 4 63kDa 0 3 0 0 Ras GTPase-activating-like protein IQGAP1 OS = Homo 189 kDa 0 135 0 0 sapiens GN = IQGAP1 PE = 1 SV = 1 Ras-related protein Rab-10OS = Homo sapiens 23 kDa 0 13 0 0 GN = RAB10 PE = 1 SV = 1 Ras-relatedprotein Rab-14 (Fragment) OS = Homo 20 kDa 0 8 0 0 sapiens GN = RAB14 PE= 1 SV = 1 Ras-related protein Rab-2A OS = Homo sapiens 24 kDa 0 5 0 1GN = RAB2A PE = 1 SV = 1 Ras-related protein Ral-A OS = Homo sapiens GN= RALA 24 kDa 0 2 0 0 PE = 1 SV = 1 Regulation of nuclear pre-mRNAdomain-containing 37 kDa 0 2 0 0 protein 1B OS = Homo sapiens GN =RPRD1B PE = 1 SV = 1 Replication protein A 32 kDa subunit OS = Homosapiens 29 kDa 0 3 0 1 GN = RPA2 PE = 1 SV = 1 Replication protein A 70kDa DNA-binding subunit 68 kDa 0 4 0 0 OS = Homo sapiens GN = RPA1 PE =1 SV = 2 Reticulocalbin-1 OS = Homo sapiens GN = RCN1 PE = 1 39 kDa 0 70 0 SV = 1 Retinoic acid-induced protein 3 OS = Homo sapiens 40 kDa 0 00 2 OX = 9606 GN = GPRC5A PE = 1 SV = 2 Rho GTPase-activating protein 1OS = Homo sapiens 50 kDa 0 8 0 0 GN = ARHGAP1 PE = 1 SV = 1 Ribosomalprotein L19 OS = Homo sapiens GN = RPL19 23 kDa 0 21 0 0 PE = 1 SV = 1Ribosome biogenesis protein BRX1 homolog OS = Homo 41 kDa 0 5 0 3sapiens OX = 9606 GN = BRIX1 PE = 1 SV = 2 Ribosome biogenesis proteinWDR12 OS = Homo sapiens 48 kDa 0 3 0 0 GN = WDR12 PE = 1 SV = 2 Ribosomebiogenesis regulatory protein homolog 41 kDa 0 11 0 0 OS = Homo sapiensGN = RRS1 PE = 1 SV = 2 Ribosome production factor 2 homolog OS = Homo36 kDa 0 3 0 0 sapiens GN = RPF2 PE = 1 SV = 2 RNA-binding protein 28 OS= Homo sapiens OX = 9606 86 kDa 0 2 0 2 GN = RBM28 PE = 1 SV = 3RNA-binding protein 3 OS = Homo sapiens GN = RBM3 17 kDa 0 3 0 0 PE = 1SV = 1 rRNA 2′-O-methyltransferase fibrillarin OS = Homo 34 kDa 0 23 0 0sapiens GN = FBL PE = 1 SV = 2 Selenoprotein H OS = Homo sapiens GN =C11orf31 PE = 1 13 kDa 0 2 0 0 SV = 1 Semaphorin-3C OS = Homo sapiens GN= SEMA3C PE = 2 85 kDa 0 3 0 0 SV = 2 Serine protease 23 OS = Homosapiens OX = 9606 43 kDa 0 7 7 5 GN = PRSS23 PE = 1 SV = 1 Serineprotease HTRA3 OS = Homo sapiens OX = 9606 49 kDa 0 3 3 0 GN = HTRA3 PE= 1 SV = 2 Serine/threonine-protein phosphatase 2A catalytic 36 kDa 0 40 0 subunit beta isoform OS = Homo sapiens GN = PPP2CB PE = 1 SV = 1Serine/threonine-protein phosphatase PP1-beta catalytic 37 kDa 0 22 0 0subunit OS = Homo sapiens GN = PPP1CB PE = 1 SV = 3 Serpin B3 OS = Homosapiens GN = SERPINB3 PE = 1 45 kDa 0 4 0 0 SV = 2 SH3 domain-bindingprotein 1 OS = Homo sapiens 76 kDa 0 2 0 0 GN = SH3BP1 PE = 1 SV = 3Signal peptidase complex subunit 1 OS = Homo sapiens 12 kDa 0 2 0 0 GN =SPCS1 PE = 1 SV = 4 Signal peptidase complex subunit 3 OS = Homo sapiens20 kDa 0 5 0 0 GN = SPCS3 PE = 1 SV = 1 Signal recognition particle 14kDa protein OS = Homo 15 kDa 0 9 0 0 sapiens GN = SRP14 PE = 1 SV = 2Signal-induced proliferation-associated 1-like protein 2 190 kDa  0 0 02 OS = Homo sapiens OX = 9606 GN = SIPA1L2 PE = 1 SV = 2 Single-strandedDNA-binding protein, mitochondrial 17 kDa 0 24 0 0 OS = Homo sapiens GN= SSBP1 PE = 1 SV = 1 SNW domain-containing protein 1 OS = Homo sapiens61 kDa 0 4 0 0 GN = SNW1 PE = 1 SV = 1 Solute carrier family 2,facilitated glucose transporter 54 kDa 0 9 0 0 member 1 OS = Homosapiens GN = SLC2A1 PE = 1 SV = 2 SPARC (Fragment) OS = Homo sapiens GN= SPARC 17 kDa 0 2 0 0 PE = 1 SV = 1 Spermatogenesis-associatedserine-rich protein 2 60 kDa 0 2 0 0 OS = Homo sapiens GN = SPATS2 PE =1 SV = 1 Sphingosine-1-phosphate lyase 1 OS = Homo sapiens 64 kDa 0 2 00 GN = SGPL1 PE = 1 SV = 3 Splicing factor 3B subunit 2 OS = Homosapiens 98 kDa 0 7 0 0 GN = SF3B2 PE = 1 SV = 1 SRAstem-loop-interacting RNA-binding protein, 14 kDa 0 2 0 0 mitochondrialOS = Homo sapiens GN = SLIRP PE = 1 SV = 1 Structural maintenance ofchromosomes protein 3 142 kDa  0 11 0 0 OS = Homo sapiens GN = SMC3 PE =1 SV = 2 SWI/SNF-related matrix-associated actin-dependent 122 kDa  0 170 0 regulator of chromatin subfamily A member 5 OS = Homo sapiens GN =SMARCA5 PE = 1 SV = 1 SWI/SNF-related matrix-associated actin-dependent47 kDa 0 4 0 0 regulator of chromatin subfamily E member 1 OS = Homosapiens OX = 9606 GN = SMARCE1 PE = 1 SV = 2 SWISS-PROT:P01044-1 (Bostaurus) Isoform HMW of 69 kDa 0 3 0 0 Kininogen-1 precursorSWISS-PROT:P02768-1 Tax_Id = 9606 69 kDa 0 0 5 13 Gene_Symbol = ALBIsoform 1 of Serum albumin precursor SWISS-PROT:Q3SZR3 (Bos taurus)Alpha-1-acid 23 kDa 0 2 0 0 glycoprotein precursor SWISS-PROT:Q3TTY5Tax_Id = 10090 71 kDa 0 27 7 7 Gene_Symbol = Krt2 Keratin, type IIcytoskeletal 2 epidermal SWISS-PROT:Q9D312 Tax_Id = 10090 49 kDa 0 6 0 4Gene_Symbol = Krt20 Keratin, type I cytoskeletal 20 SWISS-PROT:Q9QWL7Tax_Id = 10090 48 kDa 0 54 0 16 Gene_Symbol = Krt17 Keratin, type Icytoskeletal 17 Synaptosomal-associated protein 23 OS = Homo sapiens 23kDa 0 6 0 0 GN = SNAP23 PE = 1 SV = 1 Tau-tubulin kinase 2 OS = Homosapiens OX = 9606 182 kDa  0 0 0 0 GN = TTBK2 PE = 1 SV = 1 TBC1 domainfamily member 1 (Fragment) OS = Homo 98 kDa 0 2 0 0 sapiens GN = TBC1D1PE = 1 SV = 3 T-complex protein 1 subunit alpha OS = Homo sapiens 60 kDa0 98 0 0 GN = TCP1 PE = 1 SV = 1 Thyroid hormone receptor-associatedprotein 3 109 kDa  0 11 0 0 OS = Homo sapiens GN = THRAP3 PE = 1 SV = 2Tight junction protein ZO-1 OS = Homo sapiens 188 kDa  0 3 0 0 GN = TJP1PE = 1 SV = 1 Tight junction protein ZO-2 OS = Homo sapiens 141 kDa  0 30 0 GN = TJP2 PE = 4 SV = 1 Transcription factor A, mitochondrial OS =Homo sapiens 29 kDa 0 14 1 1 OX = 9606 GN = TFAM PE = 1 SV = 1Transcription factor SOX-3 OS = Homo sapiens 45 kDa 0 2 0 0 GN = SOX3 PE= 1 SV = 2 Transforming growth factor-beta-induced protein ig-h3 75 kDa0 670 73 58 OS = Homo sapiens OX = 9606 GN = TGFBI PE = 1 SV = 1Translocator protein OS = Homo sapiens OX = 9606 19 kDa 0 0 0 3 GN =TSPO PE = 1 SV = 3 Transmembrane protein 165 OS = Homo sapiens 35 kDa 05 0 1 GN = TMEM165 PE = 1 SV = 1 TREMBL:Q0V8M9; Q9TRIO (Bos taurus)similar to 100 kDa  0 7 0 0 inter-alpha (globulin) inhibitor H3 isoform2 TREMBL:Q2KJC7; Q8HZM3 (Bos taurus) Periostin, 87 kDa 0 1 12 5osteoblast specific factor TREMBL:Q3SZH5 (Bos taurus) Similar to 45 kDa0 11 4 2 Angiotensinogen TREMBL:Q3T052; Q5EA67 (Bos taurus) Inter-alpha102 kDa  0 10 5 1 (Globulin) inhibitor H4 TREMBL:Q6ISBO Keratin, hair,basic, 4 - Homo sapiens 65 kDa 0 16 8 8 (Human). TREMBL:Q6NXH9 Tax_Id =10090 59 kDa 0 114 7 10 Gene_Symbol = Krt73 Keratin 73 TRIO andF-actin-binding protein OS = Homo sapiens 261 kDa  0 2 0 0 OX = 9606 GN= TRIOBP PE = 1 SV = 3 Tropomodulin-3 OS = Homo sapiens OX = 9606 40 kDa0 47 21 13 GN = TMOD3 PE = 1 SV = 1 Tropomyosin 1 (Alpha), isoform CRA_fOS = Homo 37 kDa 0 162 0 0 sapiens GN = TPM1 PE = 1 SV = 1 Tropomyosin 1(Alpha), isoform CRA_m OS = Homo 29 kDa 0 118 0 0 sapiens GN = TPM1 PE =1 SV = 1 Tropomyosin alpha-3 chain OS = Homo sapiens 33 kDa 0 112 0 0 GN= TPM3 PE = 1 SV = 1 Tubby-related protein 2 (Fragment) OS = Homosapiens 24 kDa 0 0 0 2 OX = 9606 GN = TULP2 PE = 4 SV = 1 Twinfilin-1 OS= Homo sapiens OX = 9606 GN = TWF1 40 kDa 0 1 1 4 PE = 1 SV = 3Tyrosine-protein kinase OS = Homo sapiens GN = YES1 61 kDa 0 7 0 0 PE =1 SV = 1 Tyrosine--tRNA ligase OS = Homo sapiens GN = YARS 44 kDa 0 6 00 PE = 1 SV = 1 U1 small nuclear ribonucleoprotein A (Fragment) 28 kDa 07 0 0 OS = Homo sapiens GN = SNRPA PE = 1 SV = 1 U3 small nucleolarribonucleoprotein protein MPP10 79 kDa 0 6 0 0 OS = Homo sapiens GN =MPHOSPH10 PE = 1 SV = 2 U3 small nucleolar RNA-associated protein 14homolog 88 kDa 0 2 0 0 A OS = Homo sapiens OX = 9606 GN = UTP14A PE = 1SV = 1 U4/U6.U5 tri-snRNP-associated protein 1 OS = Homo 90 kDa 0 4 0 0sapiens GN = SART1 PE = 1 SV = 1 UAP56-interacting factor OS = Homosapiens OX = 9606 36 kDa 0 3 0 0 GN = FYTTD1 PE = 1 SV = 3 Ubiquitincarboxyl-terminal hydrolase 24 OS = Homo 294 kDa  0 0 0 2 sapiens OX =9606 GN = USP24 PE = 1 SV = 3 Unconventional myosin-Id OS = Homo sapiensOX = 9606 116 kDa  0 0 2 0 GN = MYO1D PE = 1 SV = 2 Unconventionalmyosin-VI OS = Homo sapiens 145 kDa  0 26 0 0 GN = MYO6 PE = 1 SV = 1Unconventional myosin-XV OS = Homo sapiens 395 kDa  0 0 2 0 OX = 9606 GN= MYO15A PE = 1 SV = 2 Urokinase-type plasminogen activator OS = Homosapiens 47 kDa 0 8 0 0 GN = PLAU PE = 1 SV = 1 UV excision repairprotein RAD23 homolog B 43 kDa 0 5 0 0 OS = Homo sapiens GN = RAD23B PE= 1 SV = 1 Vacuolar protein sorting-associated protein 26A 38 kDa 0 5 03 OS = Homo sapiens OX = 9606 GN = VPS26A PE = 1 SV = 2 Very-long-chainenoyl-CoA reductase OS = Homo sapiens 36 kDa 0 6 0 0 GN = TECR PE = 1 SV= 1 Vesicle-trafficking protein SEC22b OS = Homo sapiens 25 kDa 0 6 0 0GN = SEC22B PE = 1 SV = 4 V-type proton ATPase subunit B, brain isoform57 kDa 0 16 0 0 OS = Homo sapiens GN = ATP6V1B2 PE = 1 SV = 3 V-typeproton ATPase subunit d 1 OS = Homo sapiens 45 kDa 0 22 0 0 GN =ATP6V0D1 PE = 1 SV = 1 Zinc finger protein 469 OS = Homo sapiens OX =9606 410 kDa  0 0 0 4 GN = ZNF469 PE = 2 SV = 3

Example 3—Proliferation of iPSCs on Amniotic Fluid Cell-Derived ECM

Induced pluripotent stem cells (iPSCs) were allowed to proliferate on anamniotic fluid cell-derived ECM (Matrix B) from Example 1 in cultureusing the following procedure: commercially available, cryopreservediPSCs were thawed using a water bath at 37° C. Cell suspension wasdiluted into commercially available media for stem cell proliferation(Miltenyi Biotec MACS iPS Brew) and seeded onto the ECM at approximately1,000 cells/cm² in a 6-well-plate with 2 mL of media/well. No Rockinhibitor was used. At day 1, the full volume of media was aspiratedgently from cells in culture and replaced with fresh media. Every 24hours, full media was replaced with fresh media. Once cells began toapproach confluence (as determined by brightfield microscopy), cellswere passage manually, using a sterile needle to cut large colonies intoapproximately 100 smaller colonies and then re-plate those by physicallylifting them off the dish with the sterile needle and placing them on afresh plate of the ECM. This procedure can be repeated indefinitely.

A photomicrograph showing Day 0 and Day 2 culture of iPSCs on amnioticfluid cell-derived ECM and a bone marrow cell-derived ECM is shown inFIG. 4.

A plot of iPSC colony growth curves of iPSCs cultured in the presence ofthe amniotic fluid cell-derived ECM and a bone marrow cell-derived ECMis shown in FIG. 5.

As can be seen in FIG. 4 and FIG. 5, the iPSCs proliferated in culturein the presence of the amniotic fluid cell-derived ECM, whereas iPSCscultured in the presence of a bone marrow cell-derived ECM had nogrowth.

In another study, iPSCs were plated onto an amniotic fluid cell-derivedECM (AFC-ECM). Cells were passaged two times and then RNA was collectedfor gene expression analysis. Quantitative PCR was used to determine thegene expression. The iPSCs grown on the AFC-ECM maintained pluripotencyduring the course of the experiment and maintained expression of thecore pluripotent transcription factors POU5F1 (OCT4), SOX2 and NANOG asshown in FIG. 6. The results from these studies suggest that theamniotic fluid cell-derived ECM supports pluripotent stem cellself-renewal and proliferation.

Example 4—Proliferation of Various Cell Types on Amniotic FluidCell-Derived ECM

Mesenchymal stem cells (MSCs) obtained from bone marrow, endothelialprogenitor cells (EPCs), and chondrocytes were seeded onto amnioticfluid cell-derived ECM (AF-ECM), bone marrow cell-derived ECM (BM-ECM),and tissue culture plastic (TCP) at equal seeding density in standardcommercially available media. For MSCs and chondrocytes the media wasalpha minimum essential media (aMEM) modified without phenol red,supplemented with 15% by volume fetal bovine serum, 1%antibiotic-antimycotic (anti-anti), and 1% GlutaMax. For EPCs, the mediawas Iscove's Modified Dulbecco's Medium (IMDM) Supplemented with 20%FBS, 1% anti-anti, 1% GlutaMax, and growth factors (EGF and FGF).Photomicrographs showing increased proliferation on the two ECMsrelative to TCP are shown in FIG. 7. The proliferation of the cellsafter 4 days of culture is quantified in the bar graphs in FIG. 8a ,FIG. 8b , and FIG. 8c . Surprisingly, the amniotic fluid cell-derivedECM supports increased proliferation of the MSCs (produced by bonemarrow) relative to the bone marrow cell-derived ECM. Additionally, FIG.9 shows the percentage of MSCs expressing SSEA-4 as measured by flowcytometry after 4 days of culture on each substrate. SSEA-4 expressionis elevated after culture on amniotic fluid cell-derived ECM relative tothe other substrates.

Example 5—Differentiation of MSCs Cultured on Amniotic FluidCell-Derived ECM

Mesenchymal stem cells (MSCs) obtained from human bone marrow wereseeded on amniotic fluid cell-derived ECM (AFC-ECM) at 6×10³ cells/cm²and cultured for 14 days in growth media.

To assess the adipogenic differentiation efficiency of the cells,cultures were transferred at Day 7 to adipogenic media (DMEM containing10% FBS, 0.5 mM IBMX, 10⁻⁶ M dexamethasone, 10 μM insulin, and 200 μMindomethacin), maintained for an additional 10 days, fixed with 10%formalin for 1 hour at room temperature, and then stained with Oil RedO. Adipogenesis is verified by staining the differentiated cells withthe Oil Red O. The Oil Red O stains the lipid droplets (red staining)formed within the cells following the differentiation of the MSCs toadipocytes in culture. Photomicrographs showing adipogenicdifferentiation of the MSCs on the AFC-ECM is shown in FIG. 10.

To assess osteogenic differentiation efficiency, the cultures weretransferred to osteoblast differentiation media (growth mediasupplemented with 10⁻⁷ M dexamethasone (Sigma-Aldrich, St. Louis, Mo.)and 10⁻⁴ M L-ascorbate-2-phosphate (Wako Chemicals, Richmond, Va.),maintained an additional 25 days, fixed with 10% formalin for 1 hour atroom temperature, and then stained with 1% Silver Nitrate (Von KossaStaining). Osteogenesis is verified by staining the differentiated cellswith 1% Silver Nitrate. The Silver Nitrate stains the minerals (darkstaining) deposited following the differentiation of the MSCs toosteoblasts in culture. Photomicrographs showing osteogenicdifferentiation of the MSCs on the AFC-ECM is shown in FIG. 11. Thisstudy demonstrates that stem cells can be induced to differentiate intodifferentiated cell types in culture on an amniotic fluid cell-derivedECM.

The invention claimed is:
 1. A cell-derived extracellular matrix (ECM)derived in vitro from cells isolated from amniotic fluid, wherein thecells isolated from amniotic fluid comprise fetal cells from amnionmembrane, skin, and alimentary, respiratory, and urogenital tracts; andalso comprise stem cells and wherein the amniotic fluid is obtained froma human at greater than 37 weeks of gestational age, and wherein the ECMcomprises collagen I.
 2. The cell-derived ECM of claim 1, wherein theECM further comprises laminin, collagen alpha-1 (XVIII), basementmembrane-specific heparan sulfate proteoglycan core protein, agrin,vimentin, and collagen alpha-2 (IV), and/or isoforms thereof.
 3. Thecell-derived ECM of claim 2, wherein the isoform of collagen alpha-1(XVIII) is isoform 2, and/or wherein the isoform of agrin is isoform 6.4. The cell derived ECM of claim 2, wherein the cell-derived ECM furthercomprises fibronectin and/or an isoform thereof.
 5. The cell-derived ECMof claim 1, wherein the cell-derived ECM does not contain decorin,perlecan, and/or collagen (III).
 6. The cell-derived ECM of claim 1,wherein the cell-derived ECM is decellularized.
 7. A method of producinga cell-derived extracellular matrix (ECM) comprising collagen I invitro, the method comprising: (a) isolating cells from amniotic fluid,wherein the cells isolated from amniotic fluid comprise fetal cells fromamnion membrane, skin, and alimentary, respiratory, and urogenitaltracts and also comprises stem cells, and wherein the amniotic fluid isobtained from a human at greater than 37 weeks of gestational age; (b)seeding the isolated cells onto a cell culture container or onto a cellculture container coated with a substrate; (c) adding a culture media tothe cell culture container, and (d) culturing the cells, therebyproducing a cell-derived ECK.
 8. The method of claim 7, furthercomprising (e) decellularizing the cell-derived ECM.